Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 0.2 g/l GFPHFBIin suspension and kept its high performance during prolonged subculturing.In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.
Original languageEnglish
Article number45
JournalFrontiers in Plant Science
Volume9
DOIs
Publication statusPublished - Jan 2018
MoE publication typeA1 Journal article-refereed

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recombinant proteins
tobacco
culture media
industrial applications
cells
biomass production
genetically modified organisms
clones
biomass
nutrients
animals
proteins

Keywords

  • tobacco
  • BY-2
  • recombinant protein
  • cost evaluation
  • GFP-hydrophobin I

Cite this

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title = "Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production",
abstract = "Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 0.2 g/l GFPHFBIin suspension and kept its high performance during prolonged subculturing.In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55{\%} cost reduction with regard to biomass and up to 69{\%} reduction with regard to recombinant protein production was achieved.",
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author = "Suvi H{\"a}kkinen and Lauri Reuter and Ninni Nuorti and Jussi Joensuu and Heiko Rischer and Anneli Ritala",
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language = "English",
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journal = "Frontiers in Plant Science",
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Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production. / Häkkinen, Suvi; Reuter, Lauri; Nuorti, Ninni; Joensuu, Jussi; Rischer, Heiko; Ritala, Anneli.

In: Frontiers in Plant Science, Vol. 9, 45, 01.2018.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Häkkinen, Suvi

AU - Reuter, Lauri

AU - Nuorti, Ninni

AU - Joensuu, Jussi

AU - Rischer, Heiko

AU - Ritala, Anneli

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AB - Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 0.2 g/l GFPHFBIin suspension and kept its high performance during prolonged subculturing.In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.

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