Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production

Suvi Häkkinen, Lauri Reuter, Ninni Nuorti, Jussi Joensuu, Heiko Rischer, Anneli Ritala

    Research output: Contribution to journalArticleScientificpeer-review

    30 Citations (Scopus)


    Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein–Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named ‘Hulk,’ produced 1.1 0.2 g/l GFPHFBIin suspension and kept its high performance during prolonged subculturing.In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43–55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.
    Original languageEnglish
    Article number45
    JournalFrontiers in Plant Science
    Publication statusPublished - Jan 2018
    MoE publication typeA1 Journal article-refereed


    • tobacco
    • BY-2
    • recombinant protein
    • cost evaluation
    • GFP-hydrophobin I


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