Topochemical pretreatment of wood biomass to enhance enzymatic hydrolysis of polysaccharides to sugars

H.-Y. Mou (Corresponding Author), E. Orblin, Kristiina Kruus, P. Fardim (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

The surface chemistry of milled birch and pine wood pretreated by ionic liquid, hydrothermal and hydrotropic methods, followed by enzymatic hydrolysis was studied in this work. Surface coverage by lignin was measured by X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to describe the surface chemical composition after pretreatment in detail, and the morphology after pretreatment was investigated by FE-SEM. Ionic liquid (1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride) pretreatment at room temperature made the samples swell but did not dissolve the wood. Comparing the surface coverage by lignin, both in the case of birch and pine wood, hydrotropic worked best to remove the lignin hampering enzymatic hydrolysis. ToF-SIMS supported this finding, and showed that in birch, the carbohydrates were degraded more than in pine after hydrotropic pretreatment. The glucose yield of birch was improved by hydrotropic pretreatment from 5.1% to 83.9%, more significantly than in case of pine.
Original languageEnglish
Pages (from-to)540-545
Number of pages5
JournalBioresource Technology
Volume142
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
Lignin
Polysaccharides
polysaccharide
Sugars
Ionic Liquids
hydrolysis
sugar
Wood
Biomass
Secondary ion mass spectrometry
lignin
Ionic liquids
biomass
mass spectrometry
Carbohydrates
Surface chemistry
Glucose
ion
X ray photoelectron spectroscopy

Keywords

  • biomass pretreatment
  • enzymatic hydrolysis
  • ToF-SIMS
  • topochemistry
  • XPS

Cite this

@article{216c454b1e74487fbeb7109e4b29d14d,
title = "Topochemical pretreatment of wood biomass to enhance enzymatic hydrolysis of polysaccharides to sugars",
abstract = "The surface chemistry of milled birch and pine wood pretreated by ionic liquid, hydrothermal and hydrotropic methods, followed by enzymatic hydrolysis was studied in this work. Surface coverage by lignin was measured by X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to describe the surface chemical composition after pretreatment in detail, and the morphology after pretreatment was investigated by FE-SEM. Ionic liquid (1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride) pretreatment at room temperature made the samples swell but did not dissolve the wood. Comparing the surface coverage by lignin, both in the case of birch and pine wood, hydrotropic worked best to remove the lignin hampering enzymatic hydrolysis. ToF-SIMS supported this finding, and showed that in birch, the carbohydrates were degraded more than in pine after hydrotropic pretreatment. The glucose yield of birch was improved by hydrotropic pretreatment from 5.1{\%} to 83.9{\%}, more significantly than in case of pine.",
keywords = "biomass pretreatment, enzymatic hydrolysis, ToF-SIMS, topochemistry, XPS",
author = "H.-Y. Mou and E. Orblin and Kristiina Kruus and P. Fardim",
year = "2013",
doi = "10.1016/j.biortech.2013.05.046",
language = "English",
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pages = "540--545",
journal = "Bioresource Technology",
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}

Topochemical pretreatment of wood biomass to enhance enzymatic hydrolysis of polysaccharides to sugars. / Mou, H.-Y. (Corresponding Author); Orblin, E.; Kruus, Kristiina; Fardim, P. (Corresponding Author).

In: Bioresource Technology, Vol. 142, 2013, p. 540-545.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Topochemical pretreatment of wood biomass to enhance enzymatic hydrolysis of polysaccharides to sugars

AU - Mou, H.-Y.

AU - Orblin, E.

AU - Kruus, Kristiina

AU - Fardim, P.

PY - 2013

Y1 - 2013

N2 - The surface chemistry of milled birch and pine wood pretreated by ionic liquid, hydrothermal and hydrotropic methods, followed by enzymatic hydrolysis was studied in this work. Surface coverage by lignin was measured by X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to describe the surface chemical composition after pretreatment in detail, and the morphology after pretreatment was investigated by FE-SEM. Ionic liquid (1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride) pretreatment at room temperature made the samples swell but did not dissolve the wood. Comparing the surface coverage by lignin, both in the case of birch and pine wood, hydrotropic worked best to remove the lignin hampering enzymatic hydrolysis. ToF-SIMS supported this finding, and showed that in birch, the carbohydrates were degraded more than in pine after hydrotropic pretreatment. The glucose yield of birch was improved by hydrotropic pretreatment from 5.1% to 83.9%, more significantly than in case of pine.

AB - The surface chemistry of milled birch and pine wood pretreated by ionic liquid, hydrothermal and hydrotropic methods, followed by enzymatic hydrolysis was studied in this work. Surface coverage by lignin was measured by X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to describe the surface chemical composition after pretreatment in detail, and the morphology after pretreatment was investigated by FE-SEM. Ionic liquid (1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride) pretreatment at room temperature made the samples swell but did not dissolve the wood. Comparing the surface coverage by lignin, both in the case of birch and pine wood, hydrotropic worked best to remove the lignin hampering enzymatic hydrolysis. ToF-SIMS supported this finding, and showed that in birch, the carbohydrates were degraded more than in pine after hydrotropic pretreatment. The glucose yield of birch was improved by hydrotropic pretreatment from 5.1% to 83.9%, more significantly than in case of pine.

KW - biomass pretreatment

KW - enzymatic hydrolysis

KW - ToF-SIMS

KW - topochemistry

KW - XPS

U2 - 10.1016/j.biortech.2013.05.046

DO - 10.1016/j.biortech.2013.05.046

M3 - Article

VL - 142

SP - 540

EP - 545

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -