Abstract
Original language | English |
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Publication status | Published - 2004 |
Event | 5th International Conference on Systems Biology – ICSB 2004 - Heidelberg, Germany Duration: 9 Oct 2004 → 13 Oct 2004 |
Conference
Conference | 5th International Conference on Systems Biology – ICSB 2004 |
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Abbreviated title | ICSB 2004 |
Country | Germany |
City | Heidelberg |
Period | 9/10/04 → 13/10/04 |
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Towards mitochondrial metabolomics. / Oresic, Matej; Maaheimo, Hannu; Mattila, Ismo; Rintala, Eija; Salusjärvi, Laura; Simolin, Helena; Ruohonen, Laura; Penttilä, Merja.
2004. Poster session presented at 5th International Conference on Systems Biology – ICSB 2004, Heidelberg, Germany.Research output: Contribution to conference › Conference Poster › Scientific
TY - CONF
T1 - Towards mitochondrial metabolomics
AU - Oresic, Matej
AU - Maaheimo, Hannu
AU - Mattila, Ismo
AU - Rintala, Eija
AU - Salusjärvi, Laura
AU - Simolin, Helena
AU - Ruohonen, Laura
AU - Penttilä, Merja
N1 - CA2: BEL1 BEL2 CA: BEL
PY - 2004
Y1 - 2004
N2 - Mitochondria are cellular compartments involved in energy and redox control, and as such are key compartments regulating system homeostasis and responses to environmental stimuli. Many metabolites are mediators between different signaling or metabolic pathways, and are produced only under specific environmental stress. Our aim is to study metabolic composition of mitochondria under a variety of environmental and genetic interventios, and link such information to the cell physiology. Initially, we are studying mitochiondria in S. cerevisiae. After isolating mitochondria, we pursue the analysis using LC/MS, GC/MS, and NMR approaches. New computational and statistical methods have been developed and applied to tract subtle changes in spectral data, leading to identification of compounds most descriptive of various physiological responses. As an example, figure shows that even linear dimensionality reduction method such as PCA can separate the metabolites from whole cells and mitochondria, based on 2627 peaks detected using LC/MS, with 140 peaks separating the compartments with p
AB - Mitochondria are cellular compartments involved in energy and redox control, and as such are key compartments regulating system homeostasis and responses to environmental stimuli. Many metabolites are mediators between different signaling or metabolic pathways, and are produced only under specific environmental stress. Our aim is to study metabolic composition of mitochondria under a variety of environmental and genetic interventios, and link such information to the cell physiology. Initially, we are studying mitochiondria in S. cerevisiae. After isolating mitochondria, we pursue the analysis using LC/MS, GC/MS, and NMR approaches. New computational and statistical methods have been developed and applied to tract subtle changes in spectral data, leading to identification of compounds most descriptive of various physiological responses. As an example, figure shows that even linear dimensionality reduction method such as PCA can separate the metabolites from whole cells and mitochondria, based on 2627 peaks detected using LC/MS, with 140 peaks separating the compartments with p
M3 - Conference Poster
ER -