Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili

Michael Lienemann, Michaela A. TerAvest, Juha Pekka Pitkänen, Ingmar Stuns, Merja Penttilä, Caroline M. Ajo-Franklin, Jussi Jäntti

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose-binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose-coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.

Original languageEnglish
Pages (from-to)1184-1194
Number of pages11
JournalMicrobial Biotechnology
Volume11
Issue number6
DOIs
Publication statusPublished - 1 Nov 2018
MoE publication typeA1 Journal article-refereed

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Immobilization
Escherichia coli
Biofilms
Mannose
Shewanella
Electrons
Coated materials
Signal transduction
Biosensing Techniques
Biosensors
Oxidation-Reduction
Signal Transduction
Electrodes
Enzymes
Genes
Cells
Equipment and Supplies
Substrates

Cite this

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abstract = "Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose-binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose-coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.",
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Towards patterned bioelectronics : facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili. / Lienemann, Michael; TerAvest, Michaela A.; Pitkänen, Juha Pekka; Stuns, Ingmar; Penttilä, Merja; Ajo-Franklin, Caroline M.; Jäntti, Jussi.

In: Microbial Biotechnology, Vol. 11, No. 6, 01.11.2018, p. 1184-1194.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Ajo-Franklin, Caroline M.

AU - Jäntti, Jussi

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