Toxicological evaluation of SVOCs in exhaust emissions from light-duty vehicles using different fuel alternatives under sub-freezing conditions

Semivolatile organic compounds (SVOCs) in exhaust gas, though not directly regulated by emission standards, play a crucial role in assessing both conventional and alternative fuels. Our aim is to compare the differences in and toxicological effects of SVOC exhaust emissions from conventional and alternative fuels under sub-freezing conditions. High levels of NOx, CO2 and PAHs in SVOCs were observed in DI-E2 (EN590 winter-grade diesel), with E10 (gasoline with 10% ethanol) exhibiting higher CO2 and PAH levels compared to E85 (high-blend ethanol with an 83/17% ethanol–gasoline ratio). SVOCs from DI-E6 (EN590 diesel) demonstrated significant cytotoxicity, while E10 resulted in higher inflammatory mediators and genotoxicity. Our findings show that SVOC composition and toxicity in exhaust gas differ based on the fuel type. Despite new emissions regulations reducing diesel vehicle emissions, SVOC toxicity remains unchanged. Toxicity from SVOCs in compressed natural gas and ethanol/gasoline vehicles is notable, with gasoline exhaust showing high inflammatory and genotoxic potential.