Abstract
More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxicological responses has become possible. Development of tools that enable cost- and time-efficient analysis of such signatures from large sample numbers is currently of major interest for research, drug screening and diagnostic purposes. A method named transcript analysis with aid of affinity capture (TRAC) is a novel solution hybridization and bead-based assay enabling multiplex mRNA target detection simultaneously from large sample numbers. Functionality of TRAC has been shown in a number of applications, including microbial quantification, gene expression-based monitoring of biotechnical processes, cell-based cancer marker gene screening and siRNA validation, which are reviewed here.
Original language | English |
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Pages (from-to) | 379-385 |
Number of pages | 7 |
Journal | Expert Review of Molecular Diagnostics |
Volume | 8 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2008 |
MoE publication type | A1 Journal article-refereed |
Keywords
- biotechnical process
- cancer gene marker
- gene expression
- high-content screening
- microbial quantification
- mRNA
- ribosomal RNA
- siRNA screening
- transcript analysis