Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 379-385 |
| Number of pages | 7 |
| Journal | Expert Review of Molecular Diagnostics |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- biotechnical process
- cancer gene marker
- gene expression
- high-content screening
- microbial quantification
- mRNA
- ribosomal RNA
- siRNA screening
- transcript analysis
Cite this
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TRAC in high-content gene expression analysis : Applications in microbial population studies, process biotechnology and biomedical research. / Rautio, Jari; Satokari, Reetta; Vehmaan-Kreula, P.; Serkkola, E.; Söderlund, Hans.
In: Expert Review of Molecular Diagnostics, Vol. 8, No. 4, 2008, p. 379-385.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - TRAC in high-content gene expression analysis
T2 - Applications in microbial population studies, process biotechnology and biomedical research
AU - Rautio, Jari
AU - Satokari, Reetta
AU - Vehmaan-Kreula, P.
AU - Serkkola, E.
AU - Söderlund, Hans
PY - 2008
Y1 - 2008
N2 - More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxicological responses has become possible. Development of tools that enable cost- and time-efficient analysis of such signatures from large sample numbers is currently of major interest for research, drug screening and diagnostic purposes. A method named transcript analysis with aid of affinity capture (TRAC) is a novel solution hybridization and bead-based assay enabling multiplex mRNA target detection simultaneously from large sample numbers. Functionality of TRAC has been shown in a number of applications, including microbial quantification, gene expression-based monitoring of biotechnical processes, cell-based cancer marker gene screening and siRNA validation, which are reviewed here.
AB - More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxicological responses has become possible. Development of tools that enable cost- and time-efficient analysis of such signatures from large sample numbers is currently of major interest for research, drug screening and diagnostic purposes. A method named transcript analysis with aid of affinity capture (TRAC) is a novel solution hybridization and bead-based assay enabling multiplex mRNA target detection simultaneously from large sample numbers. Functionality of TRAC has been shown in a number of applications, including microbial quantification, gene expression-based monitoring of biotechnical processes, cell-based cancer marker gene screening and siRNA validation, which are reviewed here.
KW - biotechnical process
KW - cancer gene marker
KW - gene expression
KW - high-content screening
KW - microbial quantification
KW - mRNA
KW - ribosomal RNA
KW - siRNA screening
KW - transcript analysis
U2 - 10.1586/14737159.8.4.379
DO - 10.1586/14737159.8.4.379
M3 - Article
VL - 8
SP - 379
EP - 385
JO - Expert Review of Molecular Diagnostics
JF - Expert Review of Molecular Diagnostics
SN - 1473-7159
IS - 4
ER -