Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface

Kiyohiko Igarashi (Corresponding Author), Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Tetsuaki Okamoto, Merja Penttilä, Toshio Ando, Masahiro Samejima

Research output: Contribution to journalArticleScientificpeer-review

332 Citations (Scopus)

Abstract

A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes through use of an advanced version of high-speed atomic force microscopy. Trichoderma reesei cellobiohydrolase I (Tr Cel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by means of an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a remarkable increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes.

Original languageEnglish
Pages (from-to)1279-1282
JournalScience
Volume333
Issue number6047
DOIs
Publication statusPublished - 2 Sep 2011
MoE publication typeA1 Journal article-refereed

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Cellulase
Cellulose
Cellulose 1,4-beta-Cellobiosidase
Enzymes
Trichoderma
Biofuels
Atomic Force Microscopy
Ammonia
Biomass

Cite this

Igarashi, K., Uchihashi, T., Koivula, A., Wada, M., Kimura, S., Okamoto, T., ... Samejima, M. (2011). Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science, 333(6047), 1279-1282. https://doi.org/10.1126/science.1208386
Igarashi, Kiyohiko ; Uchihashi, Takayuki ; Koivula, Anu ; Wada, Masahisa ; Kimura, Satoshi ; Okamoto, Tetsuaki ; Penttilä, Merja ; Ando, Toshio ; Samejima, Masahiro. / Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. In: Science. 2011 ; Vol. 333, No. 6047. pp. 1279-1282.
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Igarashi, K, Uchihashi, T, Koivula, A, Wada, M, Kimura, S, Okamoto, T, Penttilä, M, Ando, T & Samejima, M 2011, 'Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface', Science, vol. 333, no. 6047, pp. 1279-1282. https://doi.org/10.1126/science.1208386

Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. / Igarashi, Kiyohiko (Corresponding Author); Uchihashi, Takayuki; Koivula, Anu; Wada, Masahisa; Kimura, Satoshi; Okamoto, Tetsuaki; Penttilä, Merja; Ando, Toshio; Samejima, Masahiro.

In: Science, Vol. 333, No. 6047, 02.09.2011, p. 1279-1282.

Research output: Contribution to journalArticleScientificpeer-review

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Igarashi K, Uchihashi T, Koivula A, Wada M, Kimura S, Okamoto T et al. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science. 2011 Sep 2;333(6047):1279-1282. https://doi.org/10.1126/science.1208386