Transcription of a-glucoside transport and metabolism genes in the hybrid brewing yeast Saccharomyces pastorianus with respect to gene provenance and fermentation temperature

Brian Gibson (Corresponding Author), John Londesborough, J. Rautio, L. Mattinen, Virve Vidgren

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

The hybrid lager yeast Saccharomyces pastorianus (S. cerevisiae × S. eubayanus) contains several genes encoding proteins responsible for the uptake and metabolism of maltose and maltotriose. In many cases the genes occur as orthologues, that is, the S. cerevisiaegene exists along with the S. eubayanus gene. Prior to formation of the hybrid, these genes existed in organisms, which had been separated for tens of millions of years and were expected to show some level of genetic and functional differentiation. In this study, oligonucleotide probes were designed for TRAC analysis of transcription of the S. cerevisiae and S. eubayanus orthologues of AGT1, MALx1, MALx2 and MALx3 as well as the S. cerevisiae‐derived MPH2/3 genes within the S. pastorianus genome. Specificity of probes was validated using mRNA from S. cerevisiae and from S. eubayanus. Probes were used to analyse gene expression during 15°P wort fermentations conducted at different temperatures (10–20°C). As well as differential expression of different genes, differential expression of orthologues was also observed during fermentation. The differences suggest that, where two forms of the gene exist, either one will dominate (as with AGT1) or expression will be staggered (MALx2), possibly to maximize transport and for efficient degradation of sugars.
Original languageEnglish
Pages (from-to)23-31
Number of pages8
JournalJournal of the Institute of Brewing
Volume119
Issue number1-2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Saccharomyces
brewing
Glucosides
provenance
Fermentation
glucosides
transcription (genetics)
Yeasts
fermentation
yeasts
Temperature
metabolism
Genes
temperature
genes
Saccharomyces cerevisiae
probes (equipment)
Gene Expression
Maltose
Oligonucleotide Probes

Keywords

  • alpha-glucoside
  • hybrid
  • lager brewing yeast
  • saccharomyces eubayanus
  • saccharomyces pastorianus
  • transcription

Cite this

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title = "Transcription of a-glucoside transport and metabolism genes in the hybrid brewing yeast Saccharomyces pastorianus with respect to gene provenance and fermentation temperature",
abstract = "The hybrid lager yeast Saccharomyces pastorianus (S. cerevisiae × S. eubayanus) contains several genes encoding proteins responsible for the uptake and metabolism of maltose and maltotriose. In many cases the genes occur as orthologues, that is, the S. cerevisiaegene exists along with the S. eubayanus gene. Prior to formation of the hybrid, these genes existed in organisms, which had been separated for tens of millions of years and were expected to show some level of genetic and functional differentiation. In this study, oligonucleotide probes were designed for TRAC analysis of transcription of the S. cerevisiae and S. eubayanus orthologues of AGT1, MALx1, MALx2 and MALx3 as well as the S. cerevisiae‐derived MPH2/3 genes within the S. pastorianus genome. Specificity of probes was validated using mRNA from S. cerevisiae and from S. eubayanus. Probes were used to analyse gene expression during 15°P wort fermentations conducted at different temperatures (10–20°C). As well as differential expression of different genes, differential expression of orthologues was also observed during fermentation. The differences suggest that, where two forms of the gene exist, either one will dominate (as with AGT1) or expression will be staggered (MALx2), possibly to maximize transport and for efficient degradation of sugars.",
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author = "Brian Gibson and John Londesborough and J. Rautio and L. Mattinen and Virve Vidgren",
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Transcription of a-glucoside transport and metabolism genes in the hybrid brewing yeast Saccharomyces pastorianus with respect to gene provenance and fermentation temperature. / Gibson, Brian (Corresponding Author); Londesborough, John; Rautio, J.; Mattinen, L.; Vidgren, Virve.

In: Journal of the Institute of Brewing, Vol. 119, No. 1-2, 2013, p. 23-31.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Transcription of a-glucoside transport and metabolism genes in the hybrid brewing yeast Saccharomyces pastorianus with respect to gene provenance and fermentation temperature

AU - Gibson, Brian

AU - Londesborough, John

AU - Rautio, J.

AU - Mattinen, L.

AU - Vidgren, Virve

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N2 - The hybrid lager yeast Saccharomyces pastorianus (S. cerevisiae × S. eubayanus) contains several genes encoding proteins responsible for the uptake and metabolism of maltose and maltotriose. In many cases the genes occur as orthologues, that is, the S. cerevisiaegene exists along with the S. eubayanus gene. Prior to formation of the hybrid, these genes existed in organisms, which had been separated for tens of millions of years and were expected to show some level of genetic and functional differentiation. In this study, oligonucleotide probes were designed for TRAC analysis of transcription of the S. cerevisiae and S. eubayanus orthologues of AGT1, MALx1, MALx2 and MALx3 as well as the S. cerevisiae‐derived MPH2/3 genes within the S. pastorianus genome. Specificity of probes was validated using mRNA from S. cerevisiae and from S. eubayanus. Probes were used to analyse gene expression during 15°P wort fermentations conducted at different temperatures (10–20°C). As well as differential expression of different genes, differential expression of orthologues was also observed during fermentation. The differences suggest that, where two forms of the gene exist, either one will dominate (as with AGT1) or expression will be staggered (MALx2), possibly to maximize transport and for efficient degradation of sugars.

AB - The hybrid lager yeast Saccharomyces pastorianus (S. cerevisiae × S. eubayanus) contains several genes encoding proteins responsible for the uptake and metabolism of maltose and maltotriose. In many cases the genes occur as orthologues, that is, the S. cerevisiaegene exists along with the S. eubayanus gene. Prior to formation of the hybrid, these genes existed in organisms, which had been separated for tens of millions of years and were expected to show some level of genetic and functional differentiation. In this study, oligonucleotide probes were designed for TRAC analysis of transcription of the S. cerevisiae and S. eubayanus orthologues of AGT1, MALx1, MALx2 and MALx3 as well as the S. cerevisiae‐derived MPH2/3 genes within the S. pastorianus genome. Specificity of probes was validated using mRNA from S. cerevisiae and from S. eubayanus. Probes were used to analyse gene expression during 15°P wort fermentations conducted at different temperatures (10–20°C). As well as differential expression of different genes, differential expression of orthologues was also observed during fermentation. The differences suggest that, where two forms of the gene exist, either one will dominate (as with AGT1) or expression will be staggered (MALx2), possibly to maximize transport and for efficient degradation of sugars.

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KW - hybrid

KW - lager brewing yeast

KW - saccharomyces eubayanus

KW - saccharomyces pastorianus

KW - transcription

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