Abstract
Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains.
| Original language | English |
|---|---|
| Journal | Frontiers in Bioengineering and Biotechnology |
| Volume | 10 |
| Issue number | 901037 |
| DOIs | |
| Publication status | Published - 15 Jul 2022 |
| MoE publication type | A2 Review article in a scientific journal |
Funding
LM received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement No. 713482 (ALERT Program).
Keywords
- biosynthetic gene cluster
- fungal platform strains
- secondary metabilites
- synthetic biology
- synthetic transcriptional regulators