Abstract
Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently. Production strains have been traditionally improved
by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible
to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information
obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified
enzyme mixtures for degradation of specific types of lignocellulose materials. In this study the hydrolytic system of Trichoderma reesei Rut-C30 in the
presence of different substrates was studied by cultivating the fungus in the presence of e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce,
xylan and bagasse. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were
indentified from the data and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulose degrading enzymes were compared
in the presence of the different substrates to identify co-regulated groups of genes.
by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible
to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information
obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified
enzyme mixtures for degradation of specific types of lignocellulose materials. In this study the hydrolytic system of Trichoderma reesei Rut-C30 in the
presence of different substrates was studied by cultivating the fungus in the presence of e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce,
xylan and bagasse. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were
indentified from the data and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulose degrading enzymes were compared
in the presence of the different substrates to identify co-regulated groups of genes.
| Original language | English |
|---|---|
| Title of host publication | 26th Fungal Genetics Conference at Asilomar March 15-20, 2011 |
| Publisher | Genetics Society of America |
| Chapter | Poster Abstracts |
| Pages | 272 |
| Publication status | Published - 2011 |
| Event | 26th Fungal Genetics Conference - Asilomar, United States Duration: 15 Mar 2011 → 20 Mar 2011 |
Publication series
| Series | Fungal Genetics Reports |
|---|---|
| Number | Supplement |
| Volume | 58 |
Conference
| Conference | 26th Fungal Genetics Conference |
|---|---|
| Country/Territory | United States |
| City | Asilomar |
| Period | 15/03/11 → 20/03/11 |