Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate

Mari Häkkinen (Corresponding author), Markku Saloheimo, Merja Penttilä, Tiina Pakula

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently. Production strains have been traditionally improved
by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible
to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information
obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified
enzyme mixtures for degradation of specific types of lignocellulose materials. In this study the hydrolytic system of Trichoderma reesei Rut-C30 in the
presence of different substrates was studied by cultivating the fungus in the presence of e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce,
xylan and bagasse. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were
indentified from the data and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulose degrading enzymes were compared
in the presence of the different substrates to identify co-regulated groups of genes.
Original languageEnglish
Title of host publication26th Fungal Genetics Conference at Asilomar March 15-20, 2011
PublisherGenetics Society of America
ChapterPoster Abstracts
Pages272
Publication statusPublished - 2011
Event26th Fungal Genetics Conference - Asilomar, United States
Duration: 15 Mar 201120 Mar 2011

Publication series

NameFungal Genetics Reports
PublisherThe Genetics Society of America
NumberSupplement
Volume58

Conference

Conference26th Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period15/03/1120/03/11

Fingerprint

Trichoderma reesei
lignocellulose
fungi
genes
enzymes
bagasse
genome
xylan
wheat straw
oligonucleotides
hydrolases
genetic engineering
mutagenesis
Ascomycota
glycosides
Picea
cellulose
physiology
proteins
degradation

Cite this

Häkkinen, M., Saloheimo, M., Penttilä, M., & Pakula, T. (2011). Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate. In 26th Fungal Genetics Conference at Asilomar March 15-20, 2011 (pp. 272). [645] Genetics Society of America. Fungal Genetics Reports, No. Supplement, Vol.. 58
Häkkinen, Mari ; Saloheimo, Markku ; Penttilä, Merja ; Pakula, Tiina. / Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate. 26th Fungal Genetics Conference at Asilomar March 15-20, 2011. Genetics Society of America, 2011. pp. 272 (Fungal Genetics Reports; No. Supplement, Vol. 58).
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Häkkinen, M, Saloheimo, M, Penttilä, M & Pakula, T 2011, Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate. in 26th Fungal Genetics Conference at Asilomar March 15-20, 2011., 645, Genetics Society of America, Fungal Genetics Reports, no. Supplement, vol. 58, pp. 272, 26th Fungal Genetics Conference, Asilomar, United States, 15/03/11.

Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate. / Häkkinen, Mari (Corresponding author); Saloheimo, Markku; Penttilä, Merja; Pakula, Tiina.

26th Fungal Genetics Conference at Asilomar March 15-20, 2011. Genetics Society of America, 2011. p. 272 645 (Fungal Genetics Reports; No. Supplement, Vol. 58).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

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T1 - Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate

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AU - Saloheimo, Markku

AU - Penttilä, Merja

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N2 - Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently. Production strains have been traditionally improvedby classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possibleto utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the informationobtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modifiedenzyme mixtures for degradation of specific types of lignocellulose materials. In this study the hydrolytic system of Trichoderma reesei Rut-C30 in thepresence of different substrates was studied by cultivating the fungus in the presence of e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce,xylan and bagasse. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes wereindentified from the data and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulose degrading enzymes were comparedin the presence of the different substrates to identify co-regulated groups of genes.

AB - Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently. Production strains have been traditionally improvedby classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possibleto utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the informationobtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modifiedenzyme mixtures for degradation of specific types of lignocellulose materials. In this study the hydrolytic system of Trichoderma reesei Rut-C30 in thepresence of different substrates was studied by cultivating the fungus in the presence of e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce,xylan and bagasse. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes wereindentified from the data and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulose degrading enzymes were comparedin the presence of the different substrates to identify co-regulated groups of genes.

M3 - Conference abstract in proceedings

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BT - 26th Fungal Genetics Conference at Asilomar March 15-20, 2011

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Häkkinen M, Saloheimo M, Penttilä M, Pakula T. Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrate. In 26th Fungal Genetics Conference at Asilomar March 15-20, 2011. Genetics Society of America. 2011. p. 272. 645. (Fungal Genetics Reports; No. Supplement, Vol. 58).