Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates

Mari Häkkinen (Corresponding author), Markku Saloheimo, Tiina Pakula, Merja Penttilä

Research output: Contribution to conferenceConference PosterScientific

Abstract

Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently and which is therefore used for industrial production of cellulolytic and hemicellulolytic enzymes and heterologous proteins for applications in pulp and paper, detergent, food, textile and feed industries and in biorefinery applications. Production strains have been traditionally improved by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified enzyme mixtures for degradation of specific types of lignocellulose materials. In this study Trichoderma reesei Rut-C30 was cultivated in the presence of different lignocellulose substrates in order to study the hydrolytic system of T. reesei in the presence of the substrates. The substrates included e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce, xylan and bagasse. For bagasse, different pre-treatments were used in order to get different combinations of the inducing substances. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were indentified from the data by comparing the signal intensities between the induced samples and un-induced controls, and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulase degrading enzymes were compared in the presence of the different substrates to identify co-regulated groups of genes.
Original languageEnglish
Publication statusPublished - 2010
MoE publication typeNot Eligible
Event10th European Conference on Fungal Genetics - Amsterdam, Netherlands
Duration: 29 Mar 20101 Apr 2010

Conference

Conference10th European Conference on Fungal Genetics
CountryNetherlands
CityAmsterdam
Period29/03/101/04/10

Fingerprint

Trichoderma reesei
lignocellulose
enzymes
bagasse
genes
feed industry
biorefining
fungi
genome
proteins
textile industry
xylan
wheat straw
oligonucleotides
hydrolases
genetic engineering
mutagenesis
Ascomycota
detergents
pulp

Cite this

Häkkinen, M., Saloheimo, M., Pakula, T., & Penttilä, M. (2010). Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates. Poster session presented at 10th European Conference on Fungal Genetics, Amsterdam, Netherlands.
Häkkinen, Mari ; Saloheimo, Markku ; Pakula, Tiina ; Penttilä, Merja. / Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates. Poster session presented at 10th European Conference on Fungal Genetics, Amsterdam, Netherlands.
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Häkkinen, M, Saloheimo, M, Pakula, T & Penttilä, M 2010, 'Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates' 10th European Conference on Fungal Genetics, Amsterdam, Netherlands, 29/03/10 - 1/04/10, .

Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates. / Häkkinen, Mari (Corresponding author); Saloheimo, Markku; Pakula, Tiina; Penttilä, Merja.

2010. Poster session presented at 10th European Conference on Fungal Genetics, Amsterdam, Netherlands.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates

AU - Häkkinen, Mari

AU - Saloheimo, Markku

AU - Pakula, Tiina

AU - Penttilä, Merja

N1 - PR8.47

PY - 2010

Y1 - 2010

N2 - Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently and which is therefore used for industrial production of cellulolytic and hemicellulolytic enzymes and heterologous proteins for applications in pulp and paper, detergent, food, textile and feed industries and in biorefinery applications. Production strains have been traditionally improved by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified enzyme mixtures for degradation of specific types of lignocellulose materials. In this study Trichoderma reesei Rut-C30 was cultivated in the presence of different lignocellulose substrates in order to study the hydrolytic system of T. reesei in the presence of the substrates. The substrates included e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce, xylan and bagasse. For bagasse, different pre-treatments were used in order to get different combinations of the inducing substances. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were indentified from the data by comparing the signal intensities between the induced samples and un-induced controls, and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulase degrading enzymes were compared in the presence of the different substrates to identify co-regulated groups of genes.

AB - Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently and which is therefore used for industrial production of cellulolytic and hemicellulolytic enzymes and heterologous proteins for applications in pulp and paper, detergent, food, textile and feed industries and in biorefinery applications. Production strains have been traditionally improved by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified enzyme mixtures for degradation of specific types of lignocellulose materials. In this study Trichoderma reesei Rut-C30 was cultivated in the presence of different lignocellulose substrates in order to study the hydrolytic system of T. reesei in the presence of the substrates. The substrates included e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce, xylan and bagasse. For bagasse, different pre-treatments were used in order to get different combinations of the inducing substances. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were indentified from the data by comparing the signal intensities between the induced samples and un-induced controls, and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulase degrading enzymes were compared in the presence of the different substrates to identify co-regulated groups of genes.

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M3 - Conference Poster

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Häkkinen M, Saloheimo M, Pakula T, Penttilä M. Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates. 2010. Poster session presented at 10th European Conference on Fungal Genetics, Amsterdam, Netherlands.