Transcriptional analysis of Trichoderma reesei cultivated in the presence of different lignocellulose substrates

Mari Häkkinen (Corresponding author), Markku Saloheimo, Tiina Pakula, Merja Penttilä

    Research output: Contribution to conferenceConference PosterScientific

    Abstract

    Trichoderma reesei is a soft rot Ascomycete fungus able to secrete enzymes extremely efficiently and which is therefore used for industrial production of cellulolytic and hemicellulolytic enzymes and heterologous proteins for applications in pulp and paper, detergent, food, textile and feed industries and in biorefinery applications. Production strains have been traditionally improved by classical mutagenesis as well as by specific genetic modifications. The availability of the complete genome sequence of T. reesei has made it possible to utilise genome wide analysis methods to study physiology and protein production by the fungus at different conditions and to utilise the information obtained to develop new strains with better enzyme production qualities, such as capability for enhanced protein production or production of modified enzyme mixtures for degradation of specific types of lignocellulose materials. In this study Trichoderma reesei Rut-C30 was cultivated in the presence of different lignocellulose substrates in order to study the hydrolytic system of T. reesei in the presence of the substrates. The substrates included e.g. sophorose, cellulose, pretreated wheat straw, pretreated spruce, xylan and bagasse. For bagasse, different pre-treatments were used in order to get different combinations of the inducing substances. The cultures were subjected to transcriptional profiling using oligonucleotide microarrays. Differentially expressed genes were indentified from the data by comparing the signal intensities between the induced samples and un-induced controls, and expression profiles of glycoside hydrolase genes and other genes encoding lignocellulase degrading enzymes were compared in the presence of the different substrates to identify co-regulated groups of genes.
    Original languageEnglish
    Publication statusPublished - 2010
    MoE publication typeNot Eligible
    Event10th European Conference on Fungal Genetics - Amsterdam, Netherlands
    Duration: 29 Mar 20101 Apr 2010

    Conference

    Conference10th European Conference on Fungal Genetics
    Country/TerritoryNetherlands
    CityAmsterdam
    Period29/03/101/04/10

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