Transcriptional and genomic profiling of industrial Trichoderma reesei strains

Susanna Mäkinen, Marja Paloheimo, Tiina Pakula, Mikko Arvas, Merja Oja, Markku Saloheimo, Merja Penttilä, Jarno Kallio, Jari Vehmaanperä, Terhi Puranen

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Trichoderma reesei has a long history in industrial enzyme production because of its high protein secretion capability and easy processability. Despite its many positive features, further strain engineering is still being pursued to remove any remaining protein production and secretion bottlenecks. Systems biology methods were applied to study protein production in selected proprietary T. reesei strains. Transcriptional analysis using microarrays was performed to identify differentially expressed genes in various cultivation conditions and high-density Comparative Genomic Hybridization (CGH) was used to characterize genomic alterations in the strains. Combined data from CGH and transcriptional analyses was utilized to identify target genes for further improvement of protein production/secretion. Deletions and/or overexpressions of 16 genes involved in protein transport, post-translational modification, basic energy metabolism or regulatory functions were studied in detail.
Original languageEnglish
Title of host publicationFungal Genetics Conference
Subtitle of host publication26th Fungal Genetics Conference at Asilomar
PublisherNew Prairie Press
ChapterPoster Abstracts
Pages272
Publication statusPublished - 2011
MoE publication typeNot Eligible
Event26th Fungal Genetics Conference - Asilomar, United States
Duration: 15 Mar 201120 Mar 2011

Publication series

SeriesFungal Genetics Reports
Number2
Volume58
ISSN1941-4757

Conference

Conference26th Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period15/03/1120/03/11

Fingerprint

Trichoderma
Comparative Genomic Hybridization
Proteins
Genes
Systems Biology
Protein Transport
Post Translational Protein Processing
Microarray Analysis
Energy Metabolism
History
Enzymes

Cite this

Mäkinen, S., Paloheimo, M., Pakula, T., Arvas, M., Oja, M., Saloheimo, M., ... Puranen, T. (2011). Transcriptional and genomic profiling of industrial Trichoderma reesei strains. In Fungal Genetics Conference: 26th Fungal Genetics Conference at Asilomar (pp. 272). [643] New Prairie Press. Fungal Genetics Reports, No. 2, Vol.. 58
Mäkinen, Susanna ; Paloheimo, Marja ; Pakula, Tiina ; Arvas, Mikko ; Oja, Merja ; Saloheimo, Markku ; Penttilä, Merja ; Kallio, Jarno ; Vehmaanperä, Jari ; Puranen, Terhi. / Transcriptional and genomic profiling of industrial Trichoderma reesei strains. Fungal Genetics Conference: 26th Fungal Genetics Conference at Asilomar. New Prairie Press, 2011. pp. 272 (Fungal Genetics Reports; No. 2, Vol. 58).
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Mäkinen, S, Paloheimo, M, Pakula, T, Arvas, M, Oja, M, Saloheimo, M, Penttilä, M, Kallio, J, Vehmaanperä, J & Puranen, T 2011, Transcriptional and genomic profiling of industrial Trichoderma reesei strains. in Fungal Genetics Conference: 26th Fungal Genetics Conference at Asilomar., 643, New Prairie Press, Fungal Genetics Reports, no. 2, vol. 58, pp. 272, 26th Fungal Genetics Conference, Asilomar, United States, 15/03/11.

Transcriptional and genomic profiling of industrial Trichoderma reesei strains. / Mäkinen, Susanna; Paloheimo, Marja; Pakula, Tiina; Arvas, Mikko; Oja, Merja; Saloheimo, Markku; Penttilä, Merja; Kallio, Jarno; Vehmaanperä, Jari; Puranen, Terhi.

Fungal Genetics Conference: 26th Fungal Genetics Conference at Asilomar. New Prairie Press, 2011. p. 272 643 (Fungal Genetics Reports; No. 2, Vol. 58).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Transcriptional and genomic profiling of industrial Trichoderma reesei strains

AU - Mäkinen, Susanna

AU - Paloheimo, Marja

AU - Pakula, Tiina

AU - Arvas, Mikko

AU - Oja, Merja

AU - Saloheimo, Markku

AU - Penttilä, Merja

AU - Kallio, Jarno

AU - Vehmaanperä, Jari

AU - Puranen, Terhi

N1 - Poster abstract

PY - 2011

Y1 - 2011

N2 - Trichoderma reesei has a long history in industrial enzyme production because of its high protein secretion capability and easy processability. Despite its many positive features, further strain engineering is still being pursued to remove any remaining protein production and secretion bottlenecks. Systems biology methods were applied to study protein production in selected proprietary T. reesei strains. Transcriptional analysis using microarrays was performed to identify differentially expressed genes in various cultivation conditions and high-density Comparative Genomic Hybridization (CGH) was used to characterize genomic alterations in the strains. Combined data from CGH and transcriptional analyses was utilized to identify target genes for further improvement of protein production/secretion. Deletions and/or overexpressions of 16 genes involved in protein transport, post-translational modification, basic energy metabolism or regulatory functions were studied in detail.

AB - Trichoderma reesei has a long history in industrial enzyme production because of its high protein secretion capability and easy processability. Despite its many positive features, further strain engineering is still being pursued to remove any remaining protein production and secretion bottlenecks. Systems biology methods were applied to study protein production in selected proprietary T. reesei strains. Transcriptional analysis using microarrays was performed to identify differentially expressed genes in various cultivation conditions and high-density Comparative Genomic Hybridization (CGH) was used to characterize genomic alterations in the strains. Combined data from CGH and transcriptional analyses was utilized to identify target genes for further improvement of protein production/secretion. Deletions and/or overexpressions of 16 genes involved in protein transport, post-translational modification, basic energy metabolism or regulatory functions were studied in detail.

M3 - Conference abstract in proceedings

T3 - Fungal Genetics Reports

SP - 272

BT - Fungal Genetics Conference

PB - New Prairie Press

ER -

Mäkinen S, Paloheimo M, Pakula T, Arvas M, Oja M, Saloheimo M et al. Transcriptional and genomic profiling of industrial Trichoderma reesei strains. In Fungal Genetics Conference: 26th Fungal Genetics Conference at Asilomar. New Prairie Press. 2011. p. 272. 643. (Fungal Genetics Reports; No. 2, Vol. 58).