Transcriptional and genomic profiling of industrial Trichoderma reesei strains

Susanna Mäkinen, Marja Paloheimo, Tiina Pakula, Mikko Arvas, Merja Oja, Markku Saloheimo, Merja Penttilä, Jarno Kallio, Jari Vehmaanperä, Terhi Puranen

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Trichoderma reesei has a long history in industrial enzyme production because of its high protein secretion capability and easy processability. Despite its many positive features, further strain engineering is still being pursued to remove any remaining protein production and secretion bottlenecks. Systems biology methods were applied to study protein production in selected proprietary T. reesei strains. Transcriptional analysis using microarrays was performed to identify differentially expressed genes in various cultivation conditions and high-density Comparative Genomic Hybridization (CGH) was used to characterize genomic alterations in the strains. Combined data from CGH and transcriptional analyses was utilized to identify target genes for further improvement of protein production/secretion. Deletions and/or overexpressions of 16 genes involved in protein transport, post-translational modification, basic energy metabolism or regulatory functions were studied in detail.
    Original languageEnglish
    Title of host publicationFungal Genetics Conference
    Subtitle of host publication26th Fungal Genetics Conference at Asilomar
    PublisherNew Prairie Press
    ChapterPoster Abstracts
    Pages272
    Publication statusPublished - 2011
    MoE publication typeNot Eligible
    Event26th Fungal Genetics Conference - Asilomar, United States
    Duration: 15 Mar 201120 Mar 2011

    Publication series

    SeriesFungal Genetics Reports
    Number2
    Volume58
    ISSN1941-4757

    Conference

    Conference26th Fungal Genetics Conference
    Country/TerritoryUnited States
    CityAsilomar
    Period15/03/1120/03/11

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