Transcriptional responses of saccharomyces cerevisiae to shift from respiratory and respirofermentative to fully fermentative metabolism

Eija Rintala (Corresponding Author), Paula Jouhten, Mervi Toivari, Marilyn G. Wiebe, Hannu Maaheimo, Merja Penttilä, Laura Ruohonen

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

In industrial fermentations of Saccharomyces cerevisiae, transient changes in oxygen concentration commonly occur and it is important to understand the behavior of cells during these changes. Glucose-limited chemostat cultivations were used to study the time-dependent effect of sudden oxygen depletion on the transcriptome of S. cerevisiae cells initially in fully aerobic or oxygen-limited conditions. The overall responses to anaerobic conditions of cells initially in different conditions were very similar. Independent of initial culture conditions, transient downregulation of genes related to growth and cell proliferation, mitochondrial translation and protein import, and sulphate assimilation was seen. In addition, transient or permanent upregulation of genes related to protein degradation, and phosphate and amino acid uptake was observed in all cultures. However, only in the initially oxygen-limited cultures was a transient upregulation of genes related to fatty acid oxidation, peroxisomal biogenesis, oxidative phosphorylation, TCA cycle, response to oxidative stress, and pentose phosphate pathway observed. Furthermore, from the initially oxygen-limited conditions, a rapid response around the metabolites of upper glycolysis and the pentose phosphate pathway was seen, while from the initially fully aerobic conditions, a slower response around the pathways for utilization of respiratory carbon sources was observed.

Original languageEnglish
Pages (from-to)461-476
JournalOMICS: A Journal of Integrative Biology
Volume15
Issue number7-8
DOIs
Publication statusPublished - 1 Jul 2011
MoE publication typeA1 Journal article-refereed

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Metabolism
Yeast
Saccharomyces cerevisiae
Oxygen
Pentoses
Pentose Phosphate Pathway
Genes
Phosphates
Cell culture
Up-Regulation
Chemostats
Oxidative stress
Mitochondrial Proteins
Oxidative Phosphorylation
Cell proliferation
Glycolysis
Metabolites
Transcriptome
Fermentation
Proteolysis

Cite this

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title = "Transcriptional responses of saccharomyces cerevisiae to shift from respiratory and respirofermentative to fully fermentative metabolism",
abstract = "In industrial fermentations of Saccharomyces cerevisiae, transient changes in oxygen concentration commonly occur and it is important to understand the behavior of cells during these changes. Glucose-limited chemostat cultivations were used to study the time-dependent effect of sudden oxygen depletion on the transcriptome of S. cerevisiae cells initially in fully aerobic or oxygen-limited conditions. The overall responses to anaerobic conditions of cells initially in different conditions were very similar. Independent of initial culture conditions, transient downregulation of genes related to growth and cell proliferation, mitochondrial translation and protein import, and sulphate assimilation was seen. In addition, transient or permanent upregulation of genes related to protein degradation, and phosphate and amino acid uptake was observed in all cultures. However, only in the initially oxygen-limited cultures was a transient upregulation of genes related to fatty acid oxidation, peroxisomal biogenesis, oxidative phosphorylation, TCA cycle, response to oxidative stress, and pentose phosphate pathway observed. Furthermore, from the initially oxygen-limited conditions, a rapid response around the metabolites of upper glycolysis and the pentose phosphate pathway was seen, while from the initially fully aerobic conditions, a slower response around the pathways for utilization of respiratory carbon sources was observed.",
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Transcriptional responses of saccharomyces cerevisiae to shift from respiratory and respirofermentative to fully fermentative metabolism. / Rintala, Eija (Corresponding Author); Jouhten, Paula; Toivari, Mervi; Wiebe, Marilyn G.; Maaheimo, Hannu; Penttilä, Merja; Ruohonen, Laura.

In: OMICS: A Journal of Integrative Biology, Vol. 15, No. 7-8, 01.07.2011, p. 461-476.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Rintala, Eija

AU - Jouhten, Paula

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AU - Maaheimo, Hannu

AU - Penttilä, Merja

AU - Ruohonen, Laura

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