Transesterification of phospholipids in different reaction conditions

Annikka Mustranta, Tapani Suortti, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Transesterification of synthetic dimyristoyl phosphatidylcholine with oleic acid by commercial lipase preparations from Aspergillus niger and Rhizomucor miehei was studied in the presence and absence of solvent. A high-performance liquid chromatography method for determination of the modified phosphatidylcholine was developed. Under solvent-free conditions, transesterification could be carried out as efficiently as in toluene, and the degree of hydrolysis was lower than in toluene. Transesterification was influenced by the water content as well as by the fatty acid concentration in the reaction mixture. The optimum water content for transesterification in solvent-free reaction medium was higher than in toluene with both lipases. The yield of modified phosphatidylcholine increased, and the degree of hydrolysis decreased with increasing fatty acid concentration. The maximum yield of modified phospha-tidylcholine, 35% of the original phospholipid, was obtained with R. miehei lipase.
Original languageEnglish
Pages (from-to)1415-1419
Number of pages5
JournalJournal of the American Oil Chemists' Society
Volume71
Issue number12
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

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Transesterification
Phospholipids
Lipases
Toluene
Lipase
Phosphatidylcholines
Fatty acids
Water content
Hydrolysis
Fatty Acids
Oleic acid
Aspergillus
High performance liquid chromatography
Oleic Acid

Cite this

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title = "Transesterification of phospholipids in different reaction conditions",
abstract = "Transesterification of synthetic dimyristoyl phosphatidylcholine with oleic acid by commercial lipase preparations from Aspergillus niger and Rhizomucor miehei was studied in the presence and absence of solvent. A high-performance liquid chromatography method for determination of the modified phosphatidylcholine was developed. Under solvent-free conditions, transesterification could be carried out as efficiently as in toluene, and the degree of hydrolysis was lower than in toluene. Transesterification was influenced by the water content as well as by the fatty acid concentration in the reaction mixture. The optimum water content for transesterification in solvent-free reaction medium was higher than in toluene with both lipases. The yield of modified phosphatidylcholine increased, and the degree of hydrolysis decreased with increasing fatty acid concentration. The maximum yield of modified phospha-tidylcholine, 35{\%} of the original phospholipid, was obtained with R. miehei lipase.",
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Transesterification of phospholipids in different reaction conditions. / Mustranta, Annikka; Suortti, Tapani; Poutanen, Kaisa.

In: Journal of the American Oil Chemists' Society, Vol. 71, No. 12, 1994, p. 1415-1419.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Transesterification of phospholipids in different reaction conditions

AU - Mustranta, Annikka

AU - Suortti, Tapani

AU - Poutanen, Kaisa

N1 - Project code: BEL3013

PY - 1994

Y1 - 1994

N2 - Transesterification of synthetic dimyristoyl phosphatidylcholine with oleic acid by commercial lipase preparations from Aspergillus niger and Rhizomucor miehei was studied in the presence and absence of solvent. A high-performance liquid chromatography method for determination of the modified phosphatidylcholine was developed. Under solvent-free conditions, transesterification could be carried out as efficiently as in toluene, and the degree of hydrolysis was lower than in toluene. Transesterification was influenced by the water content as well as by the fatty acid concentration in the reaction mixture. The optimum water content for transesterification in solvent-free reaction medium was higher than in toluene with both lipases. The yield of modified phosphatidylcholine increased, and the degree of hydrolysis decreased with increasing fatty acid concentration. The maximum yield of modified phospha-tidylcholine, 35% of the original phospholipid, was obtained with R. miehei lipase.

AB - Transesterification of synthetic dimyristoyl phosphatidylcholine with oleic acid by commercial lipase preparations from Aspergillus niger and Rhizomucor miehei was studied in the presence and absence of solvent. A high-performance liquid chromatography method for determination of the modified phosphatidylcholine was developed. Under solvent-free conditions, transesterification could be carried out as efficiently as in toluene, and the degree of hydrolysis was lower than in toluene. Transesterification was influenced by the water content as well as by the fatty acid concentration in the reaction mixture. The optimum water content for transesterification in solvent-free reaction medium was higher than in toluene with both lipases. The yield of modified phosphatidylcholine increased, and the degree of hydrolysis decreased with increasing fatty acid concentration. The maximum yield of modified phospha-tidylcholine, 35% of the original phospholipid, was obtained with R. miehei lipase.

U2 - 10.1007/BF02541365

DO - 10.1007/BF02541365

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JO - Journal of the American Oil Chemists' Society

JF - Journal of the American Oil Chemists' Society

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