Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

Marjatta Salmenkallio-Marttila, Kristian Aspegren, Satu Åkerman, Ulrika Kurten, Leena Mannonen, Anneli Ritala, Teemu Teeri, Veli Kauppinen

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Abstract

Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.
Original languageEnglish
Pages (from-to)301-304
JournalPlant Cell Reports
Volume15
Issue number3-4
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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Salmenkallio-Marttila, M., Aspegren, K., Åkerman, S., Kurten, U., Mannonen, L., Ritala, A., Teeri, T., & Kauppinen, V. (1995). Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Reports, 15(3-4), 301-304. https://doi.org/10.1007/BF00193741