Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.
Salmenkallio-Marttila, M., Aspegren, K., Åkerman, S., Kurten, U., Mannonen, L., Ritala, A., Teeri, T., & Kauppinen, V. (1995). Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Reports, 15(3-4), 301-304. https://doi.org/10.1007/BF00193741