Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

Marjatta Salmenkallio-Marttila, Kristian Aspegren, Satu Åkerman, Ulrika Kurten, Leena Mannonen, Anneli Ritala, Teemu Teeri, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.
Original languageEnglish
Pages (from-to)301-304
JournalPlant Cell Reports
Volume15
Issue number3-4
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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electroporation
Hordeum vulgare
protoplasts
barley
genetically modified organisms
kanamycin kinase
assays
microspores
Southern blotting
callus
gels
screening
genomics
genetic markers
cultivars
genes

Cite this

Salmenkallio-Marttila, M., Aspegren, K., Åkerman, S., Kurten, U., Mannonen, L., Ritala, A., ... Kauppinen, V. (1995). Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Reports, 15(3-4), 301-304. https://doi.org/10.1007/BF00193741
Salmenkallio-Marttila, Marjatta ; Aspegren, Kristian ; Åkerman, Satu ; Kurten, Ulrika ; Mannonen, Leena ; Ritala, Anneli ; Teeri, Teemu ; Kauppinen, Veli. / Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. In: Plant Cell Reports. 1995 ; Vol. 15, No. 3-4. pp. 301-304.
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abstract = "Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.",
author = "Marjatta Salmenkallio-Marttila and Kristian Aspegren and Satu {\AA}kerman and Ulrika Kurten and Leena Mannonen and Anneli Ritala and Teemu Teeri and Veli Kauppinen",
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Salmenkallio-Marttila, M, Aspegren, K, Åkerman, S, Kurten, U, Mannonen, L, Ritala, A, Teeri, T & Kauppinen, V 1995, 'Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts', Plant Cell Reports, vol. 15, no. 3-4, pp. 301-304. https://doi.org/10.1007/BF00193741

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. / Salmenkallio-Marttila, Marjatta; Aspegren, Kristian; Åkerman, Satu; Kurten, Ulrika; Mannonen, Leena; Ritala, Anneli; Teeri, Teemu; Kauppinen, Veli.

In: Plant Cell Reports, Vol. 15, No. 3-4, 1995, p. 301-304.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

AU - Salmenkallio-Marttila, Marjatta

AU - Aspegren, Kristian

AU - Åkerman, Satu

AU - Kurten, Ulrika

AU - Mannonen, Leena

AU - Ritala, Anneli

AU - Teeri, Teemu

AU - Kauppinen, Veli

N1 - Project code: BEL3030

PY - 1995

Y1 - 1995

N2 - Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

AB - Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

U2 - 10.1007/BF00193741

DO - 10.1007/BF00193741

M3 - Article

VL - 15

SP - 301

EP - 304

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 3-4

ER -

Salmenkallio-Marttila M, Aspegren K, Åkerman S, Kurten U, Mannonen L, Ritala A et al. Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Reports. 1995;15(3-4):301-304. https://doi.org/10.1007/BF00193741