Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

Marjatta Salmenkallio-Marttila; Kristian Aspegren; Satu Åkerman; Ulrika Kurten; Leena Mannonen; Anneli Ritala; Teemu Teeri; Veli Kauppinen

doi:10.1007/BF00193741

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

Marjatta Salmenkallio-Marttila, Kristian Aspegren, Satu Åkerman, Ulrika Kurten, Leena Mannonen, Anneli Ritala, Teemu Teeri, Veli Kauppinen

University of Helsinki

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Abstract

Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

Original language	English
Pages (from-to)	301-304
Journal	Plant Cell Reports
Volume	15
Issue number	3-4
DOIs	https://doi.org/10.1007/BF00193741
Publication status	Published - 1995
MoE publication type	A1 Journal article-refereed

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title = "Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts",

abstract = "Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.",

author = "Marjatta Salmenkallio-Marttila and Kristian Aspegren and Satu {\AA}kerman and Ulrika Kurten and Leena Mannonen and Anneli Ritala and Teemu Teeri and Veli Kauppinen",

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year = "1995",

doi = "10.1007/BF00193741",

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T1 - Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

AU - Salmenkallio-Marttila, Marjatta

AU - Aspegren, Kristian

AU - Åkerman, Satu

AU - Kurten, Ulrika

AU - Mannonen, Leena

AU - Ritala, Anneli

AU - Teeri, Teemu

AU - Kauppinen, Veli

N1 - Project code: BEL3030

PY - 1995

Y1 - 1995

N2 - Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

AB - Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

U2 - 10.1007/BF00193741

DO - 10.1007/BF00193741

M3 - Article

SN - 0721-7714

VL - 15

SP - 301

EP - 304

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 3-4

ER -

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

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