Transgenic barley in brewing: Detection of transgene and heterologous protein levels

Anneli Ritala, Teemu Teeri, S. Marttila, Annika Wilhelmson, S. Kaur, S. Rasmussen, Anna-Maria Nuutila

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Genetically modified (GM) raw materials and products have appeared on the market. The aim was to find out through a model case whether the threshold of 1% of GM contamination in foods is detectable using the present analytical methods. Transgenic barley lines were homozygotized through microspore culture for production of doubled haploid plants. The transgenic and non-transgenic barley samples were malted, mashed and fermented. The produced malts, worts and beers were analyzed in respect to the transgene and the corresponding heterologous protein. The heterologous protein was not detectable from protein extracts of malted grains. However, presence of the heterologous enzyme in the aleurone layer and scutellum of the transgenic malt was verified by immunomicroscopy. This suggested that the heterologous protein was expressed in germinating transgenic seeds. Real-time PCR proved to be repeatable and extremely sensitive for the detection of the transgene. The threshold of 1 part in 1000 (0.1%), nicely in the range of the legal requirements, was obtainable with high confidence. Detectable foreign DNA was also obtainable from wort, but repeated trials of DNA extraction from beer were not successful.
Original languageEnglish
Title of host publication9th International Barley Genetics Symposium
Subtitle of host publicationBook of Abstracts and CD-ROM Proceedings
EditorsJ. Spunar, J. Janikova
PublisherInstitute of Agricultural and Food Information
Pages487-492
ISBN (Print)978-80-90254-59-6
Publication statusPublished - 2004
MoE publication typeA4 Article in a conference publication
Event9th International Barley Genetics Symposium - Brno, Czech Republic
Duration: 20 Jun 200426 Jun 2004
Conference number: 9

Publication series

SeriesCzech Journal of Genetics and Plant Breeding
NumberSpecial Issue
Volume40
ISSN1212-1975

Conference

Conference9th International Barley Genetics Symposium
CountryCzech Republic
CityBrno
Period20/06/0426/06/04

Keywords

  • genetically modified (GM)
  • transgenic
  • doubled haploid (DH)
  • barley
  • malting
  • brewing
  • real-time PCR
  • immunomicroscopy

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