Transgenic oat for improved BYDV resistance

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Among the cereal crops cultivated in Finland oat (Avena saliva L.) ranks second, after barley. Finland is one of the major oat producers in the world and a considerable part of the harvest is exported. The Finnish oat cultivars are generally well adapted to the humid and cool growth conditions in Finland. In order to improve the Finnish oat cultivars better to meet the requirements of the food industry, modern biotechnical methods are being used. Our first aim was to develop oat varieties with resistance to BYDV (Barley Yellow Dwarf Virus) through gene transfer. Six Finnish oat cultivars were used. The genotypes Kolbu, Rhiannon, GP-1 and Avena sterilis were used as controls. Cell cultures were started from mature embryos of all of these cultivars. From two Finnish cultivars leaf bases were also used. Embryogenic tissue cultures or oat leaf bases were used as targets for gene transfer, which was accomplished using particle bombardment. The transformants from the gene transfer experiments with the BYDV resistance gene were regenerated and rooted on selection media and transferred in soil in the greenhouse. Transgenic plants carrying the BYDV resistance gene were obtained only from those embryogenic cell cultures, which were started from mature embryos of oat. The progeny of these transgenic plants was germinated and screened for the inheritance of the transgene. The germination frequency of the seeds has been good, for example ranging from 92 to 100 % in TI and in T2, respectively. The progeny carrying the BYDV resistance gene was submitted to viral resistance testing.
Original languageEnglish
Title of host publicationProceedings of the 7th International Oat Conference. Helsinki, Finland, 2004
PublisherNatural Resources Institute Finland
Pages88
Number of pages1
ISBN (Electronic)951-729-880-3
ISBN (Print)951-729-879-X
Publication statusPublished - 2004
MoE publication typeNot Eligible

Publication series

SeriesAgrifood Research Reports
Number51
ISSN1458-5073

Fingerprint

Barley yellow dwarf virus
oats
genetically modified organisms
gene transfer
cultivars
Finland
embryo (plant)
transgenic plants
cell culture
Avena sterilis
Avena (Poaceae)
greenhouse soils
genes
saliva
grain crops
transgenes
tissue culture
food industry
leaves
inheritance (genetics)

Keywords

  • oat
  • transgenic
  • BYDV resistance
  • AVENA

Cite this

Nuutila, A-M., Lehto, K., & Oksman-Caldentey, K-M. (2004). Transgenic oat for improved BYDV resistance. In Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004 (pp. 88). Natural Resources Institute Finland. Agrifood Research Reports, No. 51
Nuutila, Anna-Maria ; Lehto, Kirsi ; Oksman-Caldentey, Kirsi-Marja. / Transgenic oat for improved BYDV resistance. Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004. Natural Resources Institute Finland, 2004. pp. 88 (Agrifood Research Reports; No. 51).
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Nuutila, A-M, Lehto, K & Oksman-Caldentey, K-M 2004, Transgenic oat for improved BYDV resistance. in Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004. Natural Resources Institute Finland, Agrifood Research Reports, no. 51, pp. 88.

Transgenic oat for improved BYDV resistance. / Nuutila, Anna-Maria; Lehto, Kirsi; Oksman-Caldentey, Kirsi-Marja.

Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004. Natural Resources Institute Finland, 2004. p. 88 (Agrifood Research Reports; No. 51).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Transgenic oat for improved BYDV resistance

AU - Nuutila, Anna-Maria

AU - Lehto, Kirsi

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2004

Y1 - 2004

N2 - Among the cereal crops cultivated in Finland oat (Avena saliva L.) ranks second, after barley. Finland is one of the major oat producers in the world and a considerable part of the harvest is exported. The Finnish oat cultivars are generally well adapted to the humid and cool growth conditions in Finland. In order to improve the Finnish oat cultivars better to meet the requirements of the food industry, modern biotechnical methods are being used. Our first aim was to develop oat varieties with resistance to BYDV (Barley Yellow Dwarf Virus) through gene transfer. Six Finnish oat cultivars were used. The genotypes Kolbu, Rhiannon, GP-1 and Avena sterilis were used as controls. Cell cultures were started from mature embryos of all of these cultivars. From two Finnish cultivars leaf bases were also used. Embryogenic tissue cultures or oat leaf bases were used as targets for gene transfer, which was accomplished using particle bombardment. The transformants from the gene transfer experiments with the BYDV resistance gene were regenerated and rooted on selection media and transferred in soil in the greenhouse. Transgenic plants carrying the BYDV resistance gene were obtained only from those embryogenic cell cultures, which were started from mature embryos of oat. The progeny of these transgenic plants was germinated and screened for the inheritance of the transgene. The germination frequency of the seeds has been good, for example ranging from 92 to 100 % in TI and in T2, respectively. The progeny carrying the BYDV resistance gene was submitted to viral resistance testing.

AB - Among the cereal crops cultivated in Finland oat (Avena saliva L.) ranks second, after barley. Finland is one of the major oat producers in the world and a considerable part of the harvest is exported. The Finnish oat cultivars are generally well adapted to the humid and cool growth conditions in Finland. In order to improve the Finnish oat cultivars better to meet the requirements of the food industry, modern biotechnical methods are being used. Our first aim was to develop oat varieties with resistance to BYDV (Barley Yellow Dwarf Virus) through gene transfer. Six Finnish oat cultivars were used. The genotypes Kolbu, Rhiannon, GP-1 and Avena sterilis were used as controls. Cell cultures were started from mature embryos of all of these cultivars. From two Finnish cultivars leaf bases were also used. Embryogenic tissue cultures or oat leaf bases were used as targets for gene transfer, which was accomplished using particle bombardment. The transformants from the gene transfer experiments with the BYDV resistance gene were regenerated and rooted on selection media and transferred in soil in the greenhouse. Transgenic plants carrying the BYDV resistance gene were obtained only from those embryogenic cell cultures, which were started from mature embryos of oat. The progeny of these transgenic plants was germinated and screened for the inheritance of the transgene. The germination frequency of the seeds has been good, for example ranging from 92 to 100 % in TI and in T2, respectively. The progeny carrying the BYDV resistance gene was submitted to viral resistance testing.

KW - oat

KW - transgenic

KW - BYDV resistance

KW - AVENA

M3 - Conference abstract in proceedings

SN - 951-729-879-X

T3 - Agrifood Research Reports

SP - 88

BT - Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004

PB - Natural Resources Institute Finland

ER -

Nuutila A-M, Lehto K, Oksman-Caldentey K-M. Transgenic oat for improved BYDV resistance. In Proceedings of the 7th International Oat Conference. Helsinki, Finland, 2004. Natural Resources Institute Finland. 2004. p. 88. (Agrifood Research Reports; No. 51).