Transgenic oat plants via visual selection of cells expressing green fluorescent protein

H. Kaeppler (Corresponding Author), G. Menon, Ron Skadsen, Anna-Maria Nuutila, A. R. Carlson

Research output: Contribution to journalArticleScientificpeer-review

55 Citations (Scopus)

Abstract

New selectable markers and selection systems are needed to increase the efficiency and flexibility of plant transformation. The objective of this research was to determine if the green fluorescent protein (gfp) gene could be utilized as a visual selectable marker for transformation of oat (Avena sativa L.). A modified gfp gene was delivered into oat cells by microprojectile bombardment. Cell clusters expressing gfp were visually identified using fluorescence microscopy and physically isolated at each subculture. Eleven independent transgenic cell lines were obtained, and fertile plants regenerated from all lines. Transgene integration and expression were confirmed in transgenic plants and progeny. Transgene expression segregated in a 3 : 1 ratio in progeny of the majority of the transgenic lines.
Original languageEnglish
Pages (from-to)661 - 666
Number of pages6
JournalPlant Cell Reports
Volume19
Issue number7
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

green fluorescent protein
oats
genetically modified organisms
transgenes
Avena sativa
cells
fluorescence microscopy
transgenic plants
genes
cell lines

Keywords

  • Genetic engineering
  • Green fluorescent protein
  • Oat
  • Transformation

Cite this

Kaeppler, H. ; Menon, G. ; Skadsen, Ron ; Nuutila, Anna-Maria ; Carlson, A. R. / Transgenic oat plants via visual selection of cells expressing green fluorescent protein. In: Plant Cell Reports. 2000 ; Vol. 19, No. 7. pp. 661 - 666.
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Transgenic oat plants via visual selection of cells expressing green fluorescent protein. / Kaeppler, H. (Corresponding Author); Menon, G.; Skadsen, Ron; Nuutila, Anna-Maria; Carlson, A. R.

In: Plant Cell Reports, Vol. 19, No. 7, 2000, p. 661 - 666.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Menon, G.

AU - Skadsen, Ron

AU - Nuutila, Anna-Maria

AU - Carlson, A. R.

PY - 2000

Y1 - 2000

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KW - Genetic engineering

KW - Green fluorescent protein

KW - Oat

KW - Transformation

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