Abstract
New selectable markers and selection systems are needed to increase the
efficiency and flexibility of plant transformation. The objective of
this research was to determine if the green fluorescent protein (gfp) gene could be utilized as a visual selectable marker for transformation of oat (Avena sativa L.). A modified gfp gene was delivered into oat cells by microprojectile bombardment. Cell clusters expressing gfp
were visually identified using fluorescence microscopy and physically
isolated at each subculture. Eleven independent transgenic cell lines
were obtained, and fertile plants regenerated from all lines. Transgene
integration and expression were confirmed in transgenic plants and
progeny. Transgene expression segregated in a 3 : 1 ratio in progeny of
the majority of the transgenic lines.
| Original language | English |
|---|---|
| Pages (from-to) | 661-666 |
| Journal | Plant Cell Reports |
| Volume | 19 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2000 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- Genetic engineering
- Green fluorescent protein
- Oat
- Transformation