Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 16-22 |
| Journal | International Dairy Journal |
| Volume | 41 |
| DOIs | |
| Publication status | Published - 2015 |
| MoE publication type | A1 Journal article-refereed |
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Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids. / Nyyssölä, Antti; Miettinen, Hanna; Kontkanen, Hanna; Lille, Martina; Partanen, Riitta; Rokka, Susanna; Järvenpää, Eila; Lantto, Raija; Kruus, Kristiina.
In: International Dairy Journal, Vol. 41, 2015, p. 16-22.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids
AU - Nyyssölä, Antti
AU - Miettinen, Hanna
AU - Kontkanen, Hanna
AU - Lille, Martina
AU - Partanen, Riitta
AU - Rokka, Susanna
AU - Järvenpää, Eila
AU - Lantto, Raija
AU - Kruus, Kristiina
PY - 2015
Y1 - 2015
N2 - Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.
AB - Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.
U2 - 10.1016/j.idairyj.2014.09.003
DO - 10.1016/j.idairyj.2014.09.003
M3 - Article
VL - 41
SP - 16
EP - 22
JO - International Dairy Journal
JF - International Dairy Journal
SN - 0958-6946
ER -