Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids

Antti Nyyssölä, Hanna Miettinen, Hanna Kontkanen, Martina Lille, Riitta Partanen, Susanna Rokka, Eila Järvenpää, Raija Lantto, Kristiina Kruus

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.
Original languageEnglish
Pages (from-to)16-22
JournalInternational Dairy Journal
Volume41
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Pseudozyma antarctica
medium chain fatty acids
long chain fatty acids
Lipase
milk fat
saturated fatty acids
Milk
Hydrolysis
Fatty Acids
hydrolysis
Fats
montirelin
Thermomyces lanuginosus
Candida rugosa
firmness
Candida
fatty acid composition
fatty acids
sampling
lipids

Cite this

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title = "Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids",
abstract = "Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28{\%}, w/w) and increased the proportion of unsaturated ones (content of 52{\%}, w/w) at the degree of hydrolysis of 48{\%} (mol/mol) in relation to the unhydrolysed substrate (47{\%}, w/w, C12:0-C14:0 and 32{\%}, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.",
author = "Antti Nyyss{\"o}l{\"a} and Hanna Miettinen and Hanna Kontkanen and Martina Lille and Riitta Partanen and Susanna Rokka and Eila J{\"a}rvenp{\"a}{\"a} and Raija Lantto and Kristiina Kruus",
year = "2015",
doi = "10.1016/j.idairyj.2014.09.003",
language = "English",
volume = "41",
pages = "16--22",
journal = "International Dairy Journal",
issn = "0958-6946",
publisher = "Elsevier",

}

Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids. / Nyyssölä, Antti; Miettinen, Hanna; Kontkanen, Hanna; Lille, Martina; Partanen, Riitta; Rokka, Susanna; Järvenpää, Eila; Lantto, Raija; Kruus, Kristiina.

In: International Dairy Journal, Vol. 41, 2015, p. 16-22.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Treatment of milk fat with sn-2 specific Pseudozyma antarctica lipase A for targeted hydrolysis of saturated medium and long-chain fatty acids

AU - Nyyssölä, Antti

AU - Miettinen, Hanna

AU - Kontkanen, Hanna

AU - Lille, Martina

AU - Partanen, Riitta

AU - Rokka, Susanna

AU - Järvenpää, Eila

AU - Lantto, Raija

AU - Kruus, Kristiina

PY - 2015

Y1 - 2015

N2 - Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.

AB - Milk fat was treated with the sn-2 specific PAN-2 lipase from Pseudozyma antarctica with the aim of achieving selective removal of saturated fatty acids with chain lengths between C12 and C16, which are abundant at the sn-2 position. Hydrolysis was also carried out using the non-specific CRU-NS from Candida rugosa and the 1,3-specific TLA-1,3 from Thermomyces lanuginosus. PAN-2 treatment decreased the proportion of the target fatty acids considerably (content of 28%, w/w) and increased the proportion of unsaturated ones (content of 52%, w/w) at the degree of hydrolysis of 48% (mol/mol) in relation to the unhydrolysed substrate (47%, w/w, C12:0-C14:0 and 32%, w/w, unsaturated). The fatty acid distributions of the TLA-1,3 and CRU-NS treated samples were similar to untreated milk fat. The proportion of crystalline fat was smaller and the firmness lower in the PAN-2 treated milk fat samples than in the untreated milk fat at 5°C.

U2 - 10.1016/j.idairyj.2014.09.003

DO - 10.1016/j.idairyj.2014.09.003

M3 - Article

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JF - International Dairy Journal

SN - 0958-6946

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