Triacylglycerol fatty acid composition in diet-induced weight loss in subjects with abnormal glucose metabolism: The GENOBIN study

Ursula Schwab (Corresponding Author), Tuulikki Seppänen-Laakso, Laxman Yetukuri, Jyrki Ågren, Marjukka Kolehmainen, David E. Laaksonen, Anna-Liisa Ruskeepää, Helena Gylling, Matti Uusitupa, Matej Orešič (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Background

The effect of weight loss on different plasma lipid subclasses at the molecular level is unknown. The aim of this study was to examine whether a diet-induced weight reduction result in changes in the extended plasma lipid profiles (lipidome) in subjects with features of metabolic syndrome in a 33-week intervention.

Methodology/Principal Findings

Plasma samples of 9 subjects in the weight reduction group and 10 subjects in the control group were analyzed using mass spectrometry based lipidomic and fatty acid analyses. Body weight decreased in the weight reduction group by 7.8±2.9% (p<0.01). Most of the serum triacylglycerols and phosphatidylcholines were reduced. The decrease in triacylglycerols affected predominantly the saturated short chain fatty acids. This decrease of saturated short chain fatty acid containing triacylglycerols correlated with the increase of insulin sensitivity. However, levels of several longer chain fatty acids, including arachidonic and docosahexanoic acid, were not affected by weight loss. Levels of other lipids known to be associated with obesity such as sphingolipids and lysophosphatidylcholines were not altered by weight reduction.

Conclusions/Significance

Diet-induced weight loss caused significant changes in global lipid profiles in subjects with abnormal glucose metabolism. The observed changes may affect insulin sensitivity and glucose metabolism in these subjects.

Original languageEnglish
Article numbere2630
Number of pages10
JournalPLoS ONE
Volume3
Issue number7
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

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Reducing Diet
Nutrition
Metabolism
Weight Loss
Triglycerides
weight loss
Fatty Acids
fatty acid composition
triacylglycerols
Lipids
Glucose
glucose
metabolism
Volatile Fatty Acids
Chemical analysis
diet
Insulin
Plasmas
Lysophosphatidylcholines
Sphingolipids

Cite this

Schwab, Ursula ; Seppänen-Laakso, Tuulikki ; Yetukuri, Laxman ; Ågren, Jyrki ; Kolehmainen, Marjukka ; Laaksonen, David E. ; Ruskeepää, Anna-Liisa ; Gylling, Helena ; Uusitupa, Matti ; Orešič, Matej. / Triacylglycerol fatty acid composition in diet-induced weight loss in subjects with abnormal glucose metabolism : The GENOBIN study. In: PLoS ONE. 2008 ; Vol. 3, No. 7.
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abstract = "BackgroundThe effect of weight loss on different plasma lipid subclasses at the molecular level is unknown. The aim of this study was to examine whether a diet-induced weight reduction result in changes in the extended plasma lipid profiles (lipidome) in subjects with features of metabolic syndrome in a 33-week intervention.Methodology/Principal FindingsPlasma samples of 9 subjects in the weight reduction group and 10 subjects in the control group were analyzed using mass spectrometry based lipidomic and fatty acid analyses. Body weight decreased in the weight reduction group by 7.8±2.9{\%} (p<0.01). Most of the serum triacylglycerols and phosphatidylcholines were reduced. The decrease in triacylglycerols affected predominantly the saturated short chain fatty acids. This decrease of saturated short chain fatty acid containing triacylglycerols correlated with the increase of insulin sensitivity. However, levels of several longer chain fatty acids, including arachidonic and docosahexanoic acid, were not affected by weight loss. Levels of other lipids known to be associated with obesity such as sphingolipids and lysophosphatidylcholines were not altered by weight reduction.Conclusions/SignificanceDiet-induced weight loss caused significant changes in global lipid profiles in subjects with abnormal glucose metabolism. The observed changes may affect insulin sensitivity and glucose metabolism in these subjects.",
author = "Ursula Schwab and Tuulikki Sepp{\"a}nen-Laakso and Laxman Yetukuri and Jyrki {\AA}gren and Marjukka Kolehmainen and Laaksonen, {David E.} and Anna-Liisa Ruskeep{\"a}{\"a} and Helena Gylling and Matti Uusitupa and Matej Orešič",
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Triacylglycerol fatty acid composition in diet-induced weight loss in subjects with abnormal glucose metabolism : The GENOBIN study. / Schwab, Ursula (Corresponding Author); Seppänen-Laakso, Tuulikki; Yetukuri, Laxman; Ågren, Jyrki; Kolehmainen, Marjukka; Laaksonen, David E.; Ruskeepää, Anna-Liisa; Gylling, Helena; Uusitupa, Matti; Orešič, Matej (Corresponding Author).

In: PLoS ONE, Vol. 3, No. 7, e2630, 2008.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Triacylglycerol fatty acid composition in diet-induced weight loss in subjects with abnormal glucose metabolism

T2 - The GENOBIN study

AU - Schwab, Ursula

AU - Seppänen-Laakso, Tuulikki

AU - Yetukuri, Laxman

AU - Ågren, Jyrki

AU - Kolehmainen, Marjukka

AU - Laaksonen, David E.

AU - Ruskeepää, Anna-Liisa

AU - Gylling, Helena

AU - Uusitupa, Matti

AU - Orešič, Matej

PY - 2008

Y1 - 2008

N2 - BackgroundThe effect of weight loss on different plasma lipid subclasses at the molecular level is unknown. The aim of this study was to examine whether a diet-induced weight reduction result in changes in the extended plasma lipid profiles (lipidome) in subjects with features of metabolic syndrome in a 33-week intervention.Methodology/Principal FindingsPlasma samples of 9 subjects in the weight reduction group and 10 subjects in the control group were analyzed using mass spectrometry based lipidomic and fatty acid analyses. Body weight decreased in the weight reduction group by 7.8±2.9% (p<0.01). Most of the serum triacylglycerols and phosphatidylcholines were reduced. The decrease in triacylglycerols affected predominantly the saturated short chain fatty acids. This decrease of saturated short chain fatty acid containing triacylglycerols correlated with the increase of insulin sensitivity. However, levels of several longer chain fatty acids, including arachidonic and docosahexanoic acid, were not affected by weight loss. Levels of other lipids known to be associated with obesity such as sphingolipids and lysophosphatidylcholines were not altered by weight reduction.Conclusions/SignificanceDiet-induced weight loss caused significant changes in global lipid profiles in subjects with abnormal glucose metabolism. The observed changes may affect insulin sensitivity and glucose metabolism in these subjects.

AB - BackgroundThe effect of weight loss on different plasma lipid subclasses at the molecular level is unknown. The aim of this study was to examine whether a diet-induced weight reduction result in changes in the extended plasma lipid profiles (lipidome) in subjects with features of metabolic syndrome in a 33-week intervention.Methodology/Principal FindingsPlasma samples of 9 subjects in the weight reduction group and 10 subjects in the control group were analyzed using mass spectrometry based lipidomic and fatty acid analyses. Body weight decreased in the weight reduction group by 7.8±2.9% (p<0.01). Most of the serum triacylglycerols and phosphatidylcholines were reduced. The decrease in triacylglycerols affected predominantly the saturated short chain fatty acids. This decrease of saturated short chain fatty acid containing triacylglycerols correlated with the increase of insulin sensitivity. However, levels of several longer chain fatty acids, including arachidonic and docosahexanoic acid, were not affected by weight loss. Levels of other lipids known to be associated with obesity such as sphingolipids and lysophosphatidylcholines were not altered by weight reduction.Conclusions/SignificanceDiet-induced weight loss caused significant changes in global lipid profiles in subjects with abnormal glucose metabolism. The observed changes may affect insulin sensitivity and glucose metabolism in these subjects.

U2 - 10.1371/journal.pone.0002630

DO - 10.1371/journal.pone.0002630

M3 - Article

VL - 3

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 7

M1 - e2630

ER -