Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

T. Vasara, M. Saloheimo, S. Keränen, M. Penttilä

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61% amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3Gly20Val, rho3Thr25Asn, rho3Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalCurrent Genetics
Volume40
Issue number2
DOIs
Publication statusPublished - 24 Oct 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Yeasts
Mutation
Growth
Saccharomyces cerevisiae
Genes
Chitin
GTP-Binding Proteins
Actins
Proteins
Complementary DNA
Alleles
Phenotype
Amino Acids

Keywords

  • Exocyst
  • Filamentous fungus
  • GTP-binding protein

Cite this

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title = "Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation",
abstract = "The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61{\%} amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3Gly20Val, rho3Thr25Asn, rho3Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype.",
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Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation. / Vasara, T.; Saloheimo, M.; Keränen, S.; Penttilä, M.

In: Current Genetics, Vol. 40, No. 2, 24.10.2001, p. 119-127.

Research output: Contribution to journalArticleScientificpeer-review

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