Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

T. Vasara; M. Saloheimo; S. Keränen; M. Penttilä

doi:10.1007/s002940100245

Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

T. Vasara
, M. Saloheimo^*
, S. Keränen
, M. Penttilä

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

12 Citations (Scopus)

Abstract

The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61% amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3^Gly20Val, rho3^Thr25Asn, rho3^Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3^Gly20Val displayed wild-type growth and those expressing rho3^Thr25Asn and rho3^Asp126Ala had a loss-of-function phenotype.

Original language	English
Pages (from-to)	119-127
Number of pages	9
Journal	Current Genetics
Volume	40
Issue number	2
DOIs	https://doi.org/10.1007/s002940100245
Publication status	Published - 24 Oct 2001
MoE publication type	A1 Journal article-refereed

Funding

Acknowledgements The authors wish to thank Riitta Nurmi for skilful technical assistance. We thank Dr. P. Novick for providing sec mutants and Dr. Y. Matsui for the Rho3p-depletion strain YMR505 used in this study. This work is a part of the research programme VTT Industrial Biotechnology (Academy of Finland, Finnish Centre of Excellence Programme 2000–2005, Project No. 64330). It was also supported by a grant from the Viikki Graduate School of Biosciences.

Keywords

Exocyst
Filamentous fungus
GTP-binding protein

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title = "Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation",

abstract = "The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61\% amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3Gly20Val, rho3Thr25Asn, rho3Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype.",

keywords = "Exocyst, Filamentous fungus, GTP-binding protein",

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T1 - Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

AU - Vasara, T.

AU - Saloheimo, M.

AU - Keränen, S.

AU - Penttilä, M.

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N2 - The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61% amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3Gly20Val, rho3Thr25Asn, rho3Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype.

AB - The Trichoderma reesei gene, rho3, encoding the functional homologue of the Saccharomyces cerevisiae small GTP-binding protein Rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. The encoded protein showed 61% amino acid identity to the Rho3 protein. Rescue of the growth defect of a RHO3 disruption strain by an expression vector carrying rho3 cDNA confirmed the functional homology with the S. cerevisiae RHO3 gene. In addition, over-production of T. reesei RHOIII in this yeast strain appeared to improve the actin organization and chitin localization of the cells. Three putative mutant alleles of the T. reesei rho3 gene (rho3Gly20Val, rho3Thr25Asn, rho3Asp126Ala) were introduced into the wild-type yeast, in yeast with sec15 mutation and in yeast with Rho3p depletion. Cells expressing rho3Gly20Val displayed wild-type growth and those expressing rho3Thr25Asn and rho3Asp126Ala had a loss-of-function phenotype.

KW - Exocyst

KW - Filamentous fungus

KW - GTP-binding protein

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ER -

Trichoderma reesei rho3, a homologue of yeast RHO3, suppresses the growth defect of yeast sec15-1 mutation

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