Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing

Katariina Solin, Hannes Orelma, Maryam Borghei, Maija Vuoriluoto, Risto Koivunen, Orlando J. Rojas

    Research output: Contribution to journalArticleScientificpeer-review

    2 Citations (Scopus)

    Abstract

    Two-dimensional (hydrophilic) channels were patterned on films prepared from cellulose nanofibrils (CNF) using photolithography and inkjet printing. Such processes included UV-activated thiol-yne click coupling and inkjet-printed designs with polystyrene. The microfluidic channels were characterized (SEM, wetting, and fluid flow) and applied as platforms for biosensing. Compared to results from the click method, a better feature fidelity and flow properties were achieved with the simpler inkjet-printed channels. Human immunoglobulin G (hIgG) was used as target protein after surface modification with either bovine serum albumin (BSA), fibrinogen, or block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) (PDMAEMA-block-POEGMA copolymers). Surface plasmon resonance (SPR) and AFM imaging were used to determine their antifouling effect to prevent nonspecific hIgG binding. Confocal laser scanning microscopy revealed diffusion and adsorption traces in the channels. The results confirm an effective surface passivation of the microfluidic channels (95% reduction of hIgG adsorption and binding). The inexpensive and disposable systems proposed here allow designs with space-resolved blocking efficiency that offer a great potential in biosensing.

    Original languageEnglish
    Pages (from-to)1036-1044
    Number of pages9
    JournalBiomacromolecules
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - 11 Feb 2019
    MoE publication typeNot Eligible

    Fingerprint

    Fluidics
    Microfluidics
    Block copolymers
    Immunoglobulin G
    Adsorption
    Surface plasmon resonance
    Photolithography
    Passivation
    Polyethylene glycols
    Wetting
    Surface treatment
    Printing
    Flow of fluids
    Methyl Ethers
    Ethers
    Cellulose
    Polystyrenes
    Microscopic examination
    Bovine Serum Albumin
    Proteins

    Cite this

    Solin, K., Orelma, H., Borghei, M., Vuoriluoto, M., Koivunen, R., & Rojas, O. J. (2019). Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing. Biomacromolecules, 20(2), 1036-1044. https://doi.org/10.1021/acs.biomac.8b01656
    Solin, Katariina ; Orelma, Hannes ; Borghei, Maryam ; Vuoriluoto, Maija ; Koivunen, Risto ; Rojas, Orlando J. / Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing. In: Biomacromolecules. 2019 ; Vol. 20, No. 2. pp. 1036-1044.
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    abstract = "Two-dimensional (hydrophilic) channels were patterned on films prepared from cellulose nanofibrils (CNF) using photolithography and inkjet printing. Such processes included UV-activated thiol-yne click coupling and inkjet-printed designs with polystyrene. The microfluidic channels were characterized (SEM, wetting, and fluid flow) and applied as platforms for biosensing. Compared to results from the click method, a better feature fidelity and flow properties were achieved with the simpler inkjet-printed channels. Human immunoglobulin G (hIgG) was used as target protein after surface modification with either bovine serum albumin (BSA), fibrinogen, or block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) (PDMAEMA-block-POEGMA copolymers). Surface plasmon resonance (SPR) and AFM imaging were used to determine their antifouling effect to prevent nonspecific hIgG binding. Confocal laser scanning microscopy revealed diffusion and adsorption traces in the channels. The results confirm an effective surface passivation of the microfluidic channels (95{\%} reduction of hIgG adsorption and binding). The inexpensive and disposable systems proposed here allow designs with space-resolved blocking efficiency that offer a great potential in biosensing.",
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    Solin, K, Orelma, H, Borghei, M, Vuoriluoto, M, Koivunen, R & Rojas, OJ 2019, 'Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing', Biomacromolecules, vol. 20, no. 2, pp. 1036-1044. https://doi.org/10.1021/acs.biomac.8b01656

    Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing. / Solin, Katariina; Orelma, Hannes; Borghei, Maryam; Vuoriluoto, Maija; Koivunen, Risto; Rojas, Orlando J.

    In: Biomacromolecules, Vol. 20, No. 2, 11.02.2019, p. 1036-1044.

    Research output: Contribution to journalArticleScientificpeer-review

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    AU - Koivunen, Risto

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