Two divergent plasma membrane syntaxin-like SNAREs, nsyn1 and nsyn2, contribute to hyphal tip growth and other developmental processes in Neurospora crassa

Gagan D. Gupta (Corresponding Author), Stephen J. Free, Natalia N. Levina, Sirkka Keränen, I. Brent Heath

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Highly polarized exocytosis of vesicles at hyphal apices is an essential requirement of tip growth. This requirement may be met by the localization and/or activation of an apical SNARE-based machinery. We have cloned nsyn1 and nsyn2, SNAREs predicted to function at the plasma membrane in Neurospora crassa. Transformation of extra copies of nsyn1 into wild-type strains displayed effects consistent with quelling of nsyn1 expression, which was lethal in most transformants. All surviving transformants grew slowly, conidiated poorly, and were male sterile. In addition, antisense nsyn1 strains grew slowly, with abnormal hyphal diameters and polarity and defective conidiation. For nsyn2, several repeat induced point mutation (RIP) crosses produced no, or poorly germinating ascospores. Those that germinated produced slow-growing hyphae with abnormal branching. The defects in nsyn1 and nsyn2 mutants are consistent with differential impaired vesicle fusion in hyphal tips and other developmental stages.
Original languageEnglish
Pages (from-to)271-286
Number of pages16
JournalFungal Genetics and Biology
Volume40
Issue number3
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Qa-SNARE Proteins
SNARE Proteins
Neurospora crassa
Cell Membrane
Hyphae
Exocytosis
Growth
Point Mutation

Keywords

  • hyphal tip growth
  • fungi
  • exocytosis
  • SNARE
  • SNARE proteins

Cite this

Gupta, Gagan D. ; Free, Stephen J. ; Levina, Natalia N. ; Keränen, Sirkka ; Heath, I. Brent. / Two divergent plasma membrane syntaxin-like SNAREs, nsyn1 and nsyn2, contribute to hyphal tip growth and other developmental processes in Neurospora crassa. In: Fungal Genetics and Biology. 2003 ; Vol. 40, No. 3. pp. 271-286.
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abstract = "Highly polarized exocytosis of vesicles at hyphal apices is an essential requirement of tip growth. This requirement may be met by the localization and/or activation of an apical SNARE-based machinery. We have cloned nsyn1 and nsyn2, SNAREs predicted to function at the plasma membrane in Neurospora crassa. Transformation of extra copies of nsyn1 into wild-type strains displayed effects consistent with quelling of nsyn1 expression, which was lethal in most transformants. All surviving transformants grew slowly, conidiated poorly, and were male sterile. In addition, antisense nsyn1 strains grew slowly, with abnormal hyphal diameters and polarity and defective conidiation. For nsyn2, several repeat induced point mutation (RIP) crosses produced no, or poorly germinating ascospores. Those that germinated produced slow-growing hyphae with abnormal branching. The defects in nsyn1 and nsyn2 mutants are consistent with differential impaired vesicle fusion in hyphal tips and other developmental stages.",
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Two divergent plasma membrane syntaxin-like SNAREs, nsyn1 and nsyn2, contribute to hyphal tip growth and other developmental processes in Neurospora crassa. / Gupta, Gagan D. (Corresponding Author); Free, Stephen J.; Levina, Natalia N.; Keränen, Sirkka; Heath, I. Brent.

In: Fungal Genetics and Biology, Vol. 40, No. 3, 2003, p. 271-286.

Research output: Contribution to journalArticleScientificpeer-review

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