Two-photon polymerization of protein patterns with ND:YAG pulsed laser

Sanna Peltola, Minna Kellomäki, Jouko Viitanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.
Original languageEnglish
Title of host publicationProceedings
Subtitle of host publication27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008
PublisherLaser Institute of America
Pages93-98
ISBN (Print)978-0-9120-3512-3
Publication statusPublished - 2008
MoE publication typeA4 Article in a conference publication
Event27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 - Temecula, CA, United States
Duration: 20 Oct 200823 Oct 2008

Conference

Conference27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008
Abbreviated titleICALEO 2008
CountryUnited States
CityTemecula, CA
Period20/10/0823/10/08

Fingerprint

YAG lasers
pulsed lasers
polymerization
proteins
photons
fabrication
parabolic reflectors
tissue engineering
albumins
serums
molecules
strings
manufacturing
diodes
engineering
microscopy
fluorescence
cells
lasers

Cite this

Peltola, S., Kellomäki, M., & Viitanen, J. (2008). Two-photon polymerization of protein patterns with ND:YAG pulsed laser. In Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 (pp. 93-98). Laser Institute of America.
Peltola, Sanna ; Kellomäki, Minna ; Viitanen, Jouko. / Two-photon polymerization of protein patterns with ND:YAG pulsed laser. Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008. Laser Institute of America, 2008. pp. 93-98
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Peltola, S, Kellomäki, M & Viitanen, J 2008, Two-photon polymerization of protein patterns with ND:YAG pulsed laser. in Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008. Laser Institute of America, pp. 93-98, 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 , Temecula, CA, United States, 20/10/08.

Two-photon polymerization of protein patterns with ND:YAG pulsed laser. / Peltola, Sanna; Kellomäki, Minna; Viitanen, Jouko.

Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008. Laser Institute of America, 2008. p. 93-98.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

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AU - Kellomäki, Minna

AU - Viitanen, Jouko

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N2 - Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.

AB - Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.

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Peltola S, Kellomäki M, Viitanen J. Two-photon polymerization of protein patterns with ND:YAG pulsed laser. In Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008. Laser Institute of America. 2008. p. 93-98