Abstract
Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence
microscopy but it is also a powerful 3D microfabrication technique for
manufacturing of submicron structures and patterns of biological molecules.
TPP provides an efficient way to pattern surfaces of cell culture dishes or
tissue engineering scaffolds and to guide the growth of cultivated cells. In
this study the TPP process was utilized to crosslink two biologically relevant
proteins – avidin and bovine serum albumin. The polymerization setup included
a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was
operated at 532 nm. Submicron protein strings were fabricated by moving a
motorized X-Y-Z stage at a suitable rate along a predefined path. The width of
the protein patterns could be varied by changing the laser power and the
speed of the stage. These results show that this type of fabrication setup is
suitable for fabrication of patterns of biological molecules.
| Original language | English |
|---|---|
| Title of host publication | Proceedings |
| Subtitle of host publication | 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 |
| Publisher | Laser Institute of America |
| Pages | 93-98 |
| ISBN (Print) | 978-0-9120-3512-3 |
| Publication status | Published - 2008 |
| MoE publication type | A4 Article in a conference publication |
| Event | 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 - Temecula, CA, United States Duration: 20 Oct 2008 → 23 Oct 2008 |
Conference
| Conference | 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 |
|---|---|
| Abbreviated title | ICALEO 2008 |
| Country/Territory | United States |
| City | Temecula, CA |
| Period | 20/10/08 → 23/10/08 |