Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.
|Title of host publication||Proceedings|
|Subtitle of host publication||27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008|
|Publisher||Laser Institute of America|
|Publication status||Published - 2008|
|MoE publication type||A4 Article in a conference publication|
|Event||27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 - Temecula, CA, United States|
Duration: 20 Oct 2008 → 23 Oct 2008
|Conference||27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008|
|Abbreviated title||ICALEO 2008|
|Period||20/10/08 → 23/10/08|
Peltola, S., Kellomäki, M., & Viitanen, J. (2008). Two-photon polymerization of protein patterns with ND:YAG pulsed laser. In Proceedings: 27th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2008 (pp. 93-98). Laser Institute of America.