Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics

Heli Nygren, P Pöhö, Tuulikki Seppänen-Laakso, Ulla Lahtinen, M Oresic, T Hyötyläinen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described. The global profiling method is based on ultrahigh-performance liquid chromatography combined with quadrupole time-of-flight-mass spectrometry (UHPLC–QTOF-MS). The profiling approach is complemented by UHPLC–LTQ-Orbitrap mass spectrometry in MS n mode for de novo lipid identification.
Original languageEnglish
Pages (from-to)286-293
Number of pages7
JournalLC-GC North America
Volume32
Issue number4
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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