Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics

Heli Nygren, P Pöhö, Tuulikki Seppänen-Laakso, Ulla Lahtinen, M Oresic, T Hyötyläinen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described. The global profiling method is based on ultrahigh-performance liquid chromatography combined with quadrupole time-of-flight-mass spectrometry (UHPLC–QTOF-MS). The profiling approach is complemented by UHPLC–LTQ-Orbitrap mass spectrometry in MS n mode for de novo lipid identification.
Original languageEnglish
Pages (from-to)286-293
Number of pages7
JournalLC-GC North America
Volume32
Issue number4
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Liquid chromatography
Mass spectrometry
Lipids
Plasmalogens

Cite this

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title = "Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics",
abstract = "An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described. The global profiling method is based on ultrahigh-performance liquid chromatography combined with quadrupole time-of-flight-mass spectrometry (UHPLC–QTOF-MS). The profiling approach is complemented by UHPLC–LTQ-Orbitrap mass spectrometry in MS n mode for de novo lipid identification.",
author = "Heli Nygren and P P{\"o}h{\"o} and Tuulikki Sepp{\"a}nen-Laakso and Ulla Lahtinen and M Oresic and T Hy{\"o}tyl{\"a}inen",
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Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics. / Nygren, Heli; Pöhö, P; Seppänen-Laakso, Tuulikki; Lahtinen, Ulla; Oresic, M; Hyötyläinen, T.

In: LC-GC North America, Vol. 32, No. 4, 2014, p. 286-293.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics

AU - Nygren, Heli

AU - Pöhö, P

AU - Seppänen-Laakso, Tuulikki

AU - Lahtinen, Ulla

AU - Oresic, M

AU - Hyötyläinen, T

PY - 2014

Y1 - 2014

N2 - An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described. The global profiling method is based on ultrahigh-performance liquid chromatography combined with quadrupole time-of-flight-mass spectrometry (UHPLC–QTOF-MS). The profiling approach is complemented by UHPLC–LTQ-Orbitrap mass spectrometry in MS n mode for de novo lipid identification.

AB - An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described. The global profiling method is based on ultrahigh-performance liquid chromatography combined with quadrupole time-of-flight-mass spectrometry (UHPLC–QTOF-MS). The profiling approach is complemented by UHPLC–LTQ-Orbitrap mass spectrometry in MS n mode for de novo lipid identification.

M3 - Article

VL - 32

SP - 286

EP - 293

JO - LC-GC North America

JF - LC-GC North America

SN - 1527-5949

IS - 4

ER -