Ultraviolet Light Inactivation of Murine Norovirus and Human Norovirus GII: PCR May Overestimate the Persistence of Noroviruses Even When Combined with Pre-PCR Treatments

M Rönnqvist, A Mikkelä, P Tuominen, Satu Salo, L Maunula

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Transmission of gastroenteritis-causing noroviruses may be significant via contaminated surfaces. Measures for control, e.g. disinfection with ultraviolet irradiation (UV), are therefore necessary for interrupting this transmission. Human norovirus (HuNoV) GII.4 and Murine norovirus (MuNoV) were used to study the efficacy of UV for virus inactivation on dry glass surfaces. MuNoV inactivation was measured using viability assay and the reduction in viral RNA levels for both viruses using reverse transcription quantitative PCR (RT-QPCR). For each UV dose, two parallel sample groups were detected using RT-QPCR: one group was enzymatically pre-PCR treated with Pronase and RNAse enzymes, while the other was not treated enzymatically. In the viability assay, loss of infectivity and a 4-log reduction of MuNoV were observed when the viruses on glass slides were treated with a UV dose of 60 mJ/cm2 or higher. In the RT-QPCR assay, a steady 2-log decline of MuNoV and HuNoV RNA levels was observed when UV doses were raised from 0 to 150 mJ/cm2. A distinct difference in RNA levels of pretreated and non-pretreated samples was observed with UV doses of 450–1.8 × 103 mJ/cm2: the RNA levels of untreated samples remained over 1.0 × 10³ PCR units (pcr-u), while the RNA levels of enzyme-treated samples declined below 100 pcr-u. However, the data show a prominent difference between the persistence of MuNoV observed with the infectivity assay and that of viral RNA detected using RT-QPCR. Methods based on genome detection may overestimate norovirus persistence even when samples are pretreated before genome detection.
Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalFood and Environmental Virology
Volume6
Issue number1
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Norovirus
Ultraviolet Rays
ultraviolet radiation
inactivation
Polymerase Chain Reaction
mice
reverse transcription
irradiation
RNA
Reverse Transcription
quantitative polymerase chain reaction
Viral RNA
assays
dosage
viruses
Glass
glass
sampling
pathogenicity
viability

Keywords

  • Environmental surfaces
  • Murine norovirus
  • norovirus
  • UV irradiation

Cite this

@article{f3cab0a8830543be8acf63fe49dfc4f6,
title = "Ultraviolet Light Inactivation of Murine Norovirus and Human Norovirus GII: PCR May Overestimate the Persistence of Noroviruses Even When Combined with Pre-PCR Treatments",
abstract = "Transmission of gastroenteritis-causing noroviruses may be significant via contaminated surfaces. Measures for control, e.g. disinfection with ultraviolet irradiation (UV), are therefore necessary for interrupting this transmission. Human norovirus (HuNoV) GII.4 and Murine norovirus (MuNoV) were used to study the efficacy of UV for virus inactivation on dry glass surfaces. MuNoV inactivation was measured using viability assay and the reduction in viral RNA levels for both viruses using reverse transcription quantitative PCR (RT-QPCR). For each UV dose, two parallel sample groups were detected using RT-QPCR: one group was enzymatically pre-PCR treated with Pronase and RNAse enzymes, while the other was not treated enzymatically. In the viability assay, loss of infectivity and a 4-log reduction of MuNoV were observed when the viruses on glass slides were treated with a UV dose of 60 mJ/cm2 or higher. In the RT-QPCR assay, a steady 2-log decline of MuNoV and HuNoV RNA levels was observed when UV doses were raised from 0 to 150 mJ/cm2. A distinct difference in RNA levels of pretreated and non-pretreated samples was observed with UV doses of 450–1.8 × 103 mJ/cm2: the RNA levels of untreated samples remained over 1.0 × 10³ PCR units (pcr-u), while the RNA levels of enzyme-treated samples declined below 100 pcr-u. However, the data show a prominent difference between the persistence of MuNoV observed with the infectivity assay and that of viral RNA detected using RT-QPCR. Methods based on genome detection may overestimate norovirus persistence even when samples are pretreated before genome detection.",
keywords = "Environmental surfaces, Murine norovirus, norovirus, UV irradiation",
author = "M R{\"o}nnqvist and A Mikkel{\"a} and P Tuominen and Satu Salo and L Maunula",
year = "2014",
doi = "10.1007/s12560-013-9128-y",
language = "English",
volume = "6",
pages = "48--57",
journal = "Food and Environmental Virology",
issn = "1867-0334",
publisher = "Springer",
number = "1",

}

Ultraviolet Light Inactivation of Murine Norovirus and Human Norovirus GII : PCR May Overestimate the Persistence of Noroviruses Even When Combined with Pre-PCR Treatments. / Rönnqvist, M; Mikkelä, A; Tuominen, P; Salo, Satu; Maunula, L.

In: Food and Environmental Virology, Vol. 6, No. 1, 2014, p. 48-57.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Ultraviolet Light Inactivation of Murine Norovirus and Human Norovirus GII

T2 - PCR May Overestimate the Persistence of Noroviruses Even When Combined with Pre-PCR Treatments

AU - Rönnqvist, M

AU - Mikkelä, A

AU - Tuominen, P

AU - Salo, Satu

AU - Maunula, L

PY - 2014

Y1 - 2014

N2 - Transmission of gastroenteritis-causing noroviruses may be significant via contaminated surfaces. Measures for control, e.g. disinfection with ultraviolet irradiation (UV), are therefore necessary for interrupting this transmission. Human norovirus (HuNoV) GII.4 and Murine norovirus (MuNoV) were used to study the efficacy of UV for virus inactivation on dry glass surfaces. MuNoV inactivation was measured using viability assay and the reduction in viral RNA levels for both viruses using reverse transcription quantitative PCR (RT-QPCR). For each UV dose, two parallel sample groups were detected using RT-QPCR: one group was enzymatically pre-PCR treated with Pronase and RNAse enzymes, while the other was not treated enzymatically. In the viability assay, loss of infectivity and a 4-log reduction of MuNoV were observed when the viruses on glass slides were treated with a UV dose of 60 mJ/cm2 or higher. In the RT-QPCR assay, a steady 2-log decline of MuNoV and HuNoV RNA levels was observed when UV doses were raised from 0 to 150 mJ/cm2. A distinct difference in RNA levels of pretreated and non-pretreated samples was observed with UV doses of 450–1.8 × 103 mJ/cm2: the RNA levels of untreated samples remained over 1.0 × 10³ PCR units (pcr-u), while the RNA levels of enzyme-treated samples declined below 100 pcr-u. However, the data show a prominent difference between the persistence of MuNoV observed with the infectivity assay and that of viral RNA detected using RT-QPCR. Methods based on genome detection may overestimate norovirus persistence even when samples are pretreated before genome detection.

AB - Transmission of gastroenteritis-causing noroviruses may be significant via contaminated surfaces. Measures for control, e.g. disinfection with ultraviolet irradiation (UV), are therefore necessary for interrupting this transmission. Human norovirus (HuNoV) GII.4 and Murine norovirus (MuNoV) were used to study the efficacy of UV for virus inactivation on dry glass surfaces. MuNoV inactivation was measured using viability assay and the reduction in viral RNA levels for both viruses using reverse transcription quantitative PCR (RT-QPCR). For each UV dose, two parallel sample groups were detected using RT-QPCR: one group was enzymatically pre-PCR treated with Pronase and RNAse enzymes, while the other was not treated enzymatically. In the viability assay, loss of infectivity and a 4-log reduction of MuNoV were observed when the viruses on glass slides were treated with a UV dose of 60 mJ/cm2 or higher. In the RT-QPCR assay, a steady 2-log decline of MuNoV and HuNoV RNA levels was observed when UV doses were raised from 0 to 150 mJ/cm2. A distinct difference in RNA levels of pretreated and non-pretreated samples was observed with UV doses of 450–1.8 × 103 mJ/cm2: the RNA levels of untreated samples remained over 1.0 × 10³ PCR units (pcr-u), while the RNA levels of enzyme-treated samples declined below 100 pcr-u. However, the data show a prominent difference between the persistence of MuNoV observed with the infectivity assay and that of viral RNA detected using RT-QPCR. Methods based on genome detection may overestimate norovirus persistence even when samples are pretreated before genome detection.

KW - Environmental surfaces

KW - Murine norovirus

KW - norovirus

KW - UV irradiation

U2 - 10.1007/s12560-013-9128-y

DO - 10.1007/s12560-013-9128-y

M3 - Article

VL - 6

SP - 48

EP - 57

JO - Food and Environmental Virology

JF - Food and Environmental Virology

SN - 1867-0334

IS - 1

ER -