TY - JOUR
T1 - Unlocking the functional potential of polyploid yeasts
AU - Mozzachiodi, Simone
AU - Krogerus, Kristoffer
AU - Gibson, Brian
AU - Nicolas, Alain
AU - Liti, Gianni
N1 - Funding Information:
S.M. and G.L.: Agence Nationale de la Recherche (ANR-13-BSV6-0006-01, ANR-18-CE12-0004, ANR-15-IDEX-01, ANR-20-CE12-0020), Fondation pour la Recherche Médicale (EQU202003010413), UCA AAP Start-up Deep tech, CEFIPRA, Association pour la Recherche sur le Cancer (ARCPJA32020070002320). G.L., S.M., and A.N.: convention CIFRE 2016/0582 between Meiogenix and ANRT. K.K. and B.G.: no relevant funding. We thank D’Angiolo M. and Adekunle D. for their critical reading of the manuscript.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/5/11
Y1 - 2022/5/11
N2 - Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market.
AB - Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market.
KW - Meiosis
KW - Plant Breeding
KW - Polyploidy
KW - Saccharomyces cerevisiae Proteins/genetics
KW - Saccharomyces cerevisiae/genetics
UR - http://www.scopus.com/inward/record.url?scp=85129915439&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-30221-x
DO - 10.1038/s41467-022-30221-x
M3 - Article
C2 - 35545616
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2580
ER -