UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

Tuulikki Seppänen-Laakso; Heli Nygren; Heiko Rischer

doi:10.1007/7651_2017_109

UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

Tuulikki Seppänen-Laakso, Heli Nygren, Heiko Rischer (Corresponding Author)

Research output: Contribution to journal › Article › Scientific › peer-review

2 Citations (Scopus)

Abstract

Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

Original language	English
Pages (from-to)	223-232
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1980
DOIs	https://doi.org/10.1007/7651_2017_109
Publication status	Published - 2020
MoE publication type	A1 Journal article-refereed

Keywords

lipid classes
evaporative light-scattering detection
fatty acids
gas chromatography-mass spectrometry
transesterification
trimethylsilylation
ultra performance liquid chromatography

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10.1007/7651_2017_109

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Seppänen-Laakso, T., Nygren, H., & Rischer, H. (2020). UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods. Methods in Molecular Biology, 1980, 223-232. https://doi.org/10.1007/7651_2017_109

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