UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

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Abstract

Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
Original languageEnglish
Pages (from-to)1-10
JournalMethods in Molecular Biology
DOIs
Publication statusE-pub ahead of print - 21 Nov 2017
MoE publication typeA1 Journal article-refereed

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Sterols
Nonesterified Fatty Acids
Esters
Fatty Acids
Phytol
Glycerophospholipids
Microalgae
Lipids
Glycerides
Tocopherols
Hydroxyl Radical
Methanol

Keywords

  • lipid classes
  • evaporative light-scattering detection
  • fatty acids
  • gas chromatography-mass spectrometry
  • transesterification
  • trimethylsilylation
  • ultra performance liquid chromatography

Cite this

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title = "UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods",
abstract = "Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.",
keywords = "lipid classes, evaporative light-scattering detection, fatty acids, gas chromatography-mass spectrometry, transesterification, trimethylsilylation, ultra performance liquid chromatography",
author = "Tuulikki Sepp{\"a}nen-Laakso and Heli Nygren and Heiko Rischer",
year = "2017",
month = "11",
day = "21",
doi = "10.1007/7651_2017_109",
language = "English",
pages = "1--10",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
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T1 - UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

AU - Seppänen-Laakso, Tuulikki

AU - Nygren, Heli

AU - Rischer, Heiko

PY - 2017/11/21

Y1 - 2017/11/21

N2 - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

AB - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

KW - lipid classes

KW - evaporative light-scattering detection

KW - fatty acids

KW - gas chromatography-mass spectrometry

KW - transesterification

KW - trimethylsilylation

KW - ultra performance liquid chromatography

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DO - 10.1007/7651_2017_109

M3 - Article

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

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