UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

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    3 Citations (Scopus)

    Abstract

    Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
    Original languageEnglish
    Title of host publicationBiofuels from Algae
    Subtitle of host publicationMethods and Protocols
    EditorsKristian Spilling
    Place of PublicationNew York
    PublisherHumana Press
    Pages223-232
    Number of pages10
    ISBN (Electronic)978-1-4939-9416-8
    ISBN (Print)978-1-4939-9415-1
    DOIs
    Publication statusPublished - 2020
    MoE publication typeA3 Part of a book or another research book

    Publication series

    SeriesMethods in Molecular Biology
    Volume1980
    ISSN1064-3745

    Keywords

    • lipid classes
    • evaporative light-scattering detection
    • fatty acids
    • gas chromatography-mass spectrometry
    • transesterification
    • trimethylsilylation
    • ultra performance liquid chromatography

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