@inbook{731a2021019c4a3597a6c1024fb985c6,
title = "UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods",
abstract = "Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.",
keywords = "lipid classes, evaporative light-scattering detection, fatty acids, gas chromatography-mass spectrometry, transesterification, trimethylsilylation, ultra performance liquid chromatography",
author = "Tuulikki Sepp{\"a}nen-Laakso and Heli Nygren and Heiko Rischer",
note = "Funding Information: This project was supported by the EU-funded SEABIOTECH project (FP7-SBT-KBBE-311932, http://www.seabiotech.org/). The authors thank Dr. Marilyn Wiebe who was kindly supplying the Euglena gracilis biomass. The authors owe thanks to Airi Hyrk?as for excellent technical assistance in sample preparation and optimization of the running conditions in UPLC-ELSD analyses, especially. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media New York. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2020",
doi = "10.1007/7651_2017_109",
language = "English",
isbn = "978-1-4939-9415-1",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "223--232",
editor = "Kristian Spilling",
booktitle = "Biofuels from Algae",
address = "Germany",
}