TY - JOUR
T1 - UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods
AU - Seppänen-Laakso, Tuulikki
AU - Nygren, Heli
AU - Rischer, Heiko
N1 - Funding Information:
This project was supported by the EU-funded SEABIOTECH project (FP7-SBT-KBBE-311932, http://www.seabiotech.org/). The authors thank Dr. Marilyn Wiebe who was kindly supplying the Euglena gracilis biomass. The authors owe thanks to Airi Hyrk?as for excellent technical assistance in sample preparation and optimization of the running conditions in UPLC-ELSD analyses, especially.
Publisher Copyright:
© 2017, Springer Science+Business Media New York.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
AB - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
KW - lipid classes
KW - evaporative light-scattering detection
KW - fatty acids
KW - gas chromatography-mass spectrometry
KW - transesterification
KW - trimethylsilylation
KW - ultra performance liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=85076396900&partnerID=8YFLogxK
U2 - 10.1007/7651_2017_109
DO - 10.1007/7651_2017_109
M3 - Article
VL - 1980
SP - 223
EP - 232
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -