Abstract
Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
| Original language | English |
|---|---|
| Title of host publication | Biofuels from Algae |
| Subtitle of host publication | Methods and Protocols |
| Editors | Kristian Spilling |
| Place of Publication | New York |
| Publisher | Humana Press |
| Pages | 223-232 |
| ISBN (Electronic) | 978-1-4939-9416-8 |
| ISBN (Print) | 978-1-4939-9415-1 |
| DOIs | |
| Publication status | Published - 2020 |
| MoE publication type | A3 Part of a book or another research book |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 1980 |
| ISSN | 1064-3745 |
Funding
This project was supported by the EU-funded SEABIOTECH project (FP7-SBT-KBBE-311932, http://www.seabiotech.org/).
Keywords
- lipid classes
- evaporative light-scattering detection
- fatty acids
- gas chromatography-mass spectrometry
- transesterification
- trimethylsilylation
- ultra performance liquid chromatography