Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 223-232 |
| Number of pages | 10 |
| Journal | Methods in Molecular Biology |
| Volume | 1980 |
| DOIs | |
| Publication status | Published - 2020 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- lipid classes
- evaporative light-scattering detection
- fatty acids
- gas chromatography-mass spectrometry
- transesterification
- trimethylsilylation
- ultra performance liquid chromatography
Cite this
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UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods. / Seppänen-Laakso, Tuulikki; Nygren, Heli; Rischer, Heiko (Corresponding Author).
In: Methods in Molecular Biology, Vol. 1980, 2020, p. 223-232.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods
AU - Seppänen-Laakso, Tuulikki
AU - Nygren, Heli
AU - Rischer, Heiko
PY - 2020
Y1 - 2020
N2 - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
AB - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
KW - lipid classes
KW - evaporative light-scattering detection
KW - fatty acids
KW - gas chromatography-mass spectrometry
KW - transesterification
KW - trimethylsilylation
KW - ultra performance liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=85076396900&partnerID=8YFLogxK
U2 - 10.1007/7651_2017_109
DO - 10.1007/7651_2017_109
M3 - Article
VL - 1980
SP - 223
EP - 232
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -