UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

Tuulikki Seppänen-Laakso; Heli Nygren; Heiko Rischer

doi:10.1007/7651_2017_109

UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

Tuulikki Seppänen-Laakso
, Heli Nygren
, Heiko Rischer^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

10 Citations (Scopus)

Abstract

Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

Original language	English
Title of host publication	Biofuels from Algae
Subtitle of host publication	Methods and Protocols
Editors	Kristian Spilling
Place of Publication	New York
Publisher	Springer Nature
Pages	223-232
ISBN (Electronic)	978-1-4939-9416-8
ISBN (Print)	978-1-4939-9415-1
DOIs	https://doi.org/10.1007/7651_2017_109
Publication status	Published - 2020
MoE publication type	A3 Part of a book or another research book

Publication series

Series	Methods in Molecular Biology
Volume	1980
ISSN	1064-3745

Funding

This project was supported by the EU-funded SEABIOTECH project (FP7-SBT-KBBE-311932, http://www.seabiotech.org/).

Keywords

lipid classes
evaporative light-scattering detection
fatty acids
gas chromatography-mass spectrometry
transesterification
trimethylsilylation
ultra performance liquid chromatography

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10.1007/7651_2017_109

Cite this

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title = "UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods",

abstract = "Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.",

keywords = "lipid classes, evaporative light-scattering detection, fatty acids, gas chromatography-mass spectrometry, transesterification, trimethylsilylation, ultra performance liquid chromatography",

author = "Tuulikki Sepp{\"a}nen-Laakso and Heli Nygren and Heiko Rischer",

note = "Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media New York.",

year = "2020",

doi = "10.1007/7651\_2017\_109",

language = "English",

isbn = "978-1-4939-9415-1",

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publisher = "Springer Nature",

pages = "223--232",

editor = "Kristian Spilling",

booktitle = "Biofuels from Algae",

address = "United Kingdom",

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UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods. / Seppänen-Laakso, Tuulikki; Nygren, Heli ; Rischer, Heiko.
Biofuels from Algae: Methods and Protocols. ed. / Kristian Spilling. New York: Springer Nature, 2020. p. 223-232 (Methods in Molecular Biology, Vol. 1980).

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

TY - CHAP

T1 - UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

AU - Seppänen-Laakso, Tuulikki

AU - Nygren, Heli

AU - Rischer, Heiko

PY - 2020

Y1 - 2020

N2 - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

AB - Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.

KW - lipid classes

KW - evaporative light-scattering detection

KW - fatty acids

KW - gas chromatography-mass spectrometry

KW - transesterification

KW - trimethylsilylation

KW - ultra performance liquid chromatography

UR - https://www.scopus.com/pages/publications/85076396900

U2 - 10.1007/7651_2017_109

DO - 10.1007/7651_2017_109

M3 - Chapter or book article

SN - 978-1-4939-9415-1

T3 - Methods in Molecular Biology

SP - 223

EP - 232

BT - Biofuels from Algae

A2 - Spilling, Kristian

PB - Springer Nature

CY - New York

ER -

UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods

Abstract

Publication series

Funding

Keywords

Access to Document

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