Uptake and reduction of Se(IV) in two heterotrophic aerobic Pseudomonads strains isolated from boreal bog environment

Merja Lusa (Corresponding Author), Jenna Knuutinen, Malin Bomberg

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Selenite (Se(IV), SeO 3 2-) uptake and the effect of selenite supplement on protein synthesis was investigated in two Pseudomonas sp. strains isolated from a boreal bog. These aerobic bacteria efficiently reduced Se(IV) with intracellular reduced Se 0 observed in the cytoplasm under dark aerobic conditions. The proteome analysis of Se(IV) supplement and temperature responses by SDS-PAGE gel electrophoresis showed variations in the protein expression on the 40-60 kDa regions following these stress factors, probably through enzymes associated to oxidative stress or temperature adaptation. NO 3 -/NO 2 -/SO 4 2- addition enhanced Se(IV) uptake in both bacteria, but Se(IV) uptake sustained also under sulphur and nitrogen starvation. Our findings suggest two different transport mechanisms for Se(IV) uptake in these Pseudomonas sp. strains; a low affinity transport system up-regulated by NO 3 -/NO 2 -/SO 4 2- and a distinct Se(IV)O 3 2- regulated transport system. Following transport, Se(IV) is reduced in the cytoplasm, forming Se 0 granules, visible in TEM and verified using EDX.

Original languageEnglish
Pages (from-to)798-814
Number of pages17
JournalAIMS Microbiology
Volume3
Issue number4
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Selenious Acid
Wetlands
Pseudomonas
Cytoplasm
Aerobic Bacteria
Temperature
Proteome
Starvation
Sulfur
Electrophoresis
Polyacrylamide Gel Electrophoresis
Proteins
Oxidative Stress
Nitrogen
Gels
Bacteria
Enzymes

Keywords

  • environmental
  • metabolic processes
  • Pseudomonads
  • soil
  • ecology
  • selenium
  • selenite
  • boreal bog

Cite this

@article{28a4710d84f74e4d8cefdea0bdbe9ef8,
title = "Uptake and reduction of Se(IV) in two heterotrophic aerobic Pseudomonads strains isolated from boreal bog environment",
abstract = "Selenite (Se(IV), SeO 3 2-) uptake and the effect of selenite supplement on protein synthesis was investigated in two Pseudomonas sp. strains isolated from a boreal bog. These aerobic bacteria efficiently reduced Se(IV) with intracellular reduced Se 0 observed in the cytoplasm under dark aerobic conditions. The proteome analysis of Se(IV) supplement and temperature responses by SDS-PAGE gel electrophoresis showed variations in the protein expression on the 40-60 kDa regions following these stress factors, probably through enzymes associated to oxidative stress or temperature adaptation. NO 3 -/NO 2 -/SO 4 2- addition enhanced Se(IV) uptake in both bacteria, but Se(IV) uptake sustained also under sulphur and nitrogen starvation. Our findings suggest two different transport mechanisms for Se(IV) uptake in these Pseudomonas sp. strains; a low affinity transport system up-regulated by NO 3 -/NO 2 -/SO 4 2- and a distinct Se(IV)O 3 2- regulated transport system. Following transport, Se(IV) is reduced in the cytoplasm, forming Se 0 granules, visible in TEM and verified using EDX.",
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author = "Merja Lusa and Jenna Knuutinen and Malin Bomberg",
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doi = "10.3934/microbiol.2017.4.798",
language = "English",
volume = "3",
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Uptake and reduction of Se(IV) in two heterotrophic aerobic Pseudomonads strains isolated from boreal bog environment. / Lusa, Merja (Corresponding Author); Knuutinen, Jenna; Bomberg, Malin.

In: AIMS Microbiology, Vol. 3, No. 4, 2017, p. 798-814.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Uptake and reduction of Se(IV) in two heterotrophic aerobic Pseudomonads strains isolated from boreal bog environment

AU - Lusa, Merja

AU - Knuutinen, Jenna

AU - Bomberg, Malin

PY - 2017

Y1 - 2017

N2 - Selenite (Se(IV), SeO 3 2-) uptake and the effect of selenite supplement on protein synthesis was investigated in two Pseudomonas sp. strains isolated from a boreal bog. These aerobic bacteria efficiently reduced Se(IV) with intracellular reduced Se 0 observed in the cytoplasm under dark aerobic conditions. The proteome analysis of Se(IV) supplement and temperature responses by SDS-PAGE gel electrophoresis showed variations in the protein expression on the 40-60 kDa regions following these stress factors, probably through enzymes associated to oxidative stress or temperature adaptation. NO 3 -/NO 2 -/SO 4 2- addition enhanced Se(IV) uptake in both bacteria, but Se(IV) uptake sustained also under sulphur and nitrogen starvation. Our findings suggest two different transport mechanisms for Se(IV) uptake in these Pseudomonas sp. strains; a low affinity transport system up-regulated by NO 3 -/NO 2 -/SO 4 2- and a distinct Se(IV)O 3 2- regulated transport system. Following transport, Se(IV) is reduced in the cytoplasm, forming Se 0 granules, visible in TEM and verified using EDX.

AB - Selenite (Se(IV), SeO 3 2-) uptake and the effect of selenite supplement on protein synthesis was investigated in two Pseudomonas sp. strains isolated from a boreal bog. These aerobic bacteria efficiently reduced Se(IV) with intracellular reduced Se 0 observed in the cytoplasm under dark aerobic conditions. The proteome analysis of Se(IV) supplement and temperature responses by SDS-PAGE gel electrophoresis showed variations in the protein expression on the 40-60 kDa regions following these stress factors, probably through enzymes associated to oxidative stress or temperature adaptation. NO 3 -/NO 2 -/SO 4 2- addition enhanced Se(IV) uptake in both bacteria, but Se(IV) uptake sustained also under sulphur and nitrogen starvation. Our findings suggest two different transport mechanisms for Se(IV) uptake in these Pseudomonas sp. strains; a low affinity transport system up-regulated by NO 3 -/NO 2 -/SO 4 2- and a distinct Se(IV)O 3 2- regulated transport system. Following transport, Se(IV) is reduced in the cytoplasm, forming Se 0 granules, visible in TEM and verified using EDX.

KW - environmental

KW - metabolic processes

KW - Pseudomonads

KW - soil

KW - ecology

KW - selenium

KW - selenite

KW - boreal bog

U2 - 10.3934/microbiol.2017.4.798

DO - 10.3934/microbiol.2017.4.798

M3 - Article

VL - 3

SP - 798

EP - 814

JO - AIMS Microbiology

JF - AIMS Microbiology

SN - 2471-1888

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