Uptake of radioiodide by Paenibacillus sp., Pseudomonas sp., Burkholderia sp. and Rhodococcus sp. isolated from a boreal nutrient-poor bog

Merja Lusa, Jukka Lehto, Hanna Aromaa, Jenna Knuutinen, Malin Bomberg

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

Radionuclides, like radioiodine (129I), may escape deep geological nuclear waste repositories and migrate to the surface ecosystems. In surface ecosystems, microorganisms can affect their movement. Iodide uptake of six bacterial strains belonging to the genera Paenibacillus, Pseudomonas, Burkholderia and Rhodococcus isolated from an acidic boreal nutrient-poor bog was tested. The tests were run in four different growth media at three temperatures. All bacterial strains removed iodide from the solution with the highest efficiency shown by one of the Paenibacillus strains with > 99% of iodide removed from the solution in one of the used growth media. Pseudomonas, Rhodococcus and one of the two Paenibacillus strains showed highest iodide uptake in 1% yeast extract with maximum values for the distribution coefficient (Kd) ranging from 90 to 270 L/kg DW. The Burkholderia strain showed highest uptake in 1% Tryptone (maximum Kd 170 L/kg DW). The Paenibacillus strain V0-1-LW showed exceptionally high uptake in 0.5% peptone + 0.25% yeast extract broth (maximum Kd > 1,000,000 L/kg DW). Addition of 0.1% glucose to the 0.5% peptone + 0.25% yeast extract broth reduced iodide uptake at 4°C and 20°C and enhanced iodide uptake at 37°C compared to the uptake without glucose. This indicates that the uptake of glucose and iodide may be competing processes in these bacteria. We estimated that in in situ conditions of the bog, the bacterial uptake of iodide accounts for approximately 0.1%-0.3% of the total sorption of iodide in the surface, subsurface peat, gyttja and clay layers.
Original languageEnglish
Pages (from-to)26-37
JournalJournal of Environmental Sciences
Volume44
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

iodide
Iodides
bog
Nutrients
nutrient
Yeast
Glucose
Ecosystems
yeast
Peptones
glucose
Peat
Radioactive wastes
Radioisotopes
Microorganisms
gyttja
Radioactive Waste
Sorption
Bacteria
Clay

Keywords

  • Radioiodide
  • Uptake
  • Paenibacillus
  • Pseudomonas
  • Burkholderia
  • Rhodococcus

Cite this

@article{ba14dc05ed8d4689b828327f9bba07bf,
title = "Uptake of radioiodide by Paenibacillus sp., Pseudomonas sp., Burkholderia sp. and Rhodococcus sp. isolated from a boreal nutrient-poor bog",
abstract = "Radionuclides, like radioiodine (129I), may escape deep geological nuclear waste repositories and migrate to the surface ecosystems. In surface ecosystems, microorganisms can affect their movement. Iodide uptake of six bacterial strains belonging to the genera Paenibacillus, Pseudomonas, Burkholderia and Rhodococcus isolated from an acidic boreal nutrient-poor bog was tested. The tests were run in four different growth media at three temperatures. All bacterial strains removed iodide from the solution with the highest efficiency shown by one of the Paenibacillus strains with > 99{\%} of iodide removed from the solution in one of the used growth media. Pseudomonas, Rhodococcus and one of the two Paenibacillus strains showed highest iodide uptake in 1{\%} yeast extract with maximum values for the distribution coefficient (Kd) ranging from 90 to 270 L/kg DW. The Burkholderia strain showed highest uptake in 1{\%} Tryptone (maximum Kd 170 L/kg DW). The Paenibacillus strain V0-1-LW showed exceptionally high uptake in 0.5{\%} peptone + 0.25{\%} yeast extract broth (maximum Kd > 1,000,000 L/kg DW). Addition of 0.1{\%} glucose to the 0.5{\%} peptone + 0.25{\%} yeast extract broth reduced iodide uptake at 4°C and 20°C and enhanced iodide uptake at 37°C compared to the uptake without glucose. This indicates that the uptake of glucose and iodide may be competing processes in these bacteria. We estimated that in in situ conditions of the bog, the bacterial uptake of iodide accounts for approximately 0.1{\%}-0.3{\%} of the total sorption of iodide in the surface, subsurface peat, gyttja and clay layers.",
keywords = "Radioiodide, Uptake, Paenibacillus, Pseudomonas, Burkholderia, Rhodococcus",
author = "Merja Lusa and Jukka Lehto and Hanna Aromaa and Jenna Knuutinen and Malin Bomberg",
year = "2016",
doi = "10.1016/j.jes.2015.08.026",
language = "English",
volume = "44",
pages = "26--37",
journal = "Journal of Environmental Sciences",
issn = "1001-0742",
publisher = "Elsevier",

}

Uptake of radioiodide by Paenibacillus sp., Pseudomonas sp., Burkholderia sp. and Rhodococcus sp. isolated from a boreal nutrient-poor bog. / Lusa, Merja; Lehto, Jukka; Aromaa, Hanna; Knuutinen, Jenna; Bomberg, Malin.

In: Journal of Environmental Sciences, Vol. 44, 2016, p. 26-37.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Uptake of radioiodide by Paenibacillus sp., Pseudomonas sp., Burkholderia sp. and Rhodococcus sp. isolated from a boreal nutrient-poor bog

AU - Lusa, Merja

AU - Lehto, Jukka

AU - Aromaa, Hanna

AU - Knuutinen, Jenna

AU - Bomberg, Malin

PY - 2016

Y1 - 2016

N2 - Radionuclides, like radioiodine (129I), may escape deep geological nuclear waste repositories and migrate to the surface ecosystems. In surface ecosystems, microorganisms can affect their movement. Iodide uptake of six bacterial strains belonging to the genera Paenibacillus, Pseudomonas, Burkholderia and Rhodococcus isolated from an acidic boreal nutrient-poor bog was tested. The tests were run in four different growth media at three temperatures. All bacterial strains removed iodide from the solution with the highest efficiency shown by one of the Paenibacillus strains with > 99% of iodide removed from the solution in one of the used growth media. Pseudomonas, Rhodococcus and one of the two Paenibacillus strains showed highest iodide uptake in 1% yeast extract with maximum values for the distribution coefficient (Kd) ranging from 90 to 270 L/kg DW. The Burkholderia strain showed highest uptake in 1% Tryptone (maximum Kd 170 L/kg DW). The Paenibacillus strain V0-1-LW showed exceptionally high uptake in 0.5% peptone + 0.25% yeast extract broth (maximum Kd > 1,000,000 L/kg DW). Addition of 0.1% glucose to the 0.5% peptone + 0.25% yeast extract broth reduced iodide uptake at 4°C and 20°C and enhanced iodide uptake at 37°C compared to the uptake without glucose. This indicates that the uptake of glucose and iodide may be competing processes in these bacteria. We estimated that in in situ conditions of the bog, the bacterial uptake of iodide accounts for approximately 0.1%-0.3% of the total sorption of iodide in the surface, subsurface peat, gyttja and clay layers.

AB - Radionuclides, like radioiodine (129I), may escape deep geological nuclear waste repositories and migrate to the surface ecosystems. In surface ecosystems, microorganisms can affect their movement. Iodide uptake of six bacterial strains belonging to the genera Paenibacillus, Pseudomonas, Burkholderia and Rhodococcus isolated from an acidic boreal nutrient-poor bog was tested. The tests were run in four different growth media at three temperatures. All bacterial strains removed iodide from the solution with the highest efficiency shown by one of the Paenibacillus strains with > 99% of iodide removed from the solution in one of the used growth media. Pseudomonas, Rhodococcus and one of the two Paenibacillus strains showed highest iodide uptake in 1% yeast extract with maximum values for the distribution coefficient (Kd) ranging from 90 to 270 L/kg DW. The Burkholderia strain showed highest uptake in 1% Tryptone (maximum Kd 170 L/kg DW). The Paenibacillus strain V0-1-LW showed exceptionally high uptake in 0.5% peptone + 0.25% yeast extract broth (maximum Kd > 1,000,000 L/kg DW). Addition of 0.1% glucose to the 0.5% peptone + 0.25% yeast extract broth reduced iodide uptake at 4°C and 20°C and enhanced iodide uptake at 37°C compared to the uptake without glucose. This indicates that the uptake of glucose and iodide may be competing processes in these bacteria. We estimated that in in situ conditions of the bog, the bacterial uptake of iodide accounts for approximately 0.1%-0.3% of the total sorption of iodide in the surface, subsurface peat, gyttja and clay layers.

KW - Radioiodide

KW - Uptake

KW - Paenibacillus

KW - Pseudomonas

KW - Burkholderia

KW - Rhodococcus

U2 - 10.1016/j.jes.2015.08.026

DO - 10.1016/j.jes.2015.08.026

M3 - Article

VL - 44

SP - 26

EP - 37

JO - Journal of Environmental Sciences

JF - Journal of Environmental Sciences

SN - 1001-0742

ER -