Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation

Michael Bailey (Corresponding Author), Bernhard Adamitsch, Jari Rautio, Niklas von Weymarn, Markku Saloheimo

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

In this work, a thermostable laccase from the fungus Melanocarpus albomyces was produced in the efficient protein producer Trichoderma reesei in batch, fed-batch and continuous cultivations on lactose medium in a laboratory fermenter. The rate of consumption of base for pH control (DELTABAS) was measured in all the cultivations and was used as a control parameter in one fed-batch run and in a 1000 h continuous cultivation. The dynamic DELTABAS control parameter can be used to maintain optimal production of secreted protein by fitting the rate of substrate feeding to the amount of substrate available to the growing fungus in the fermenter, thus maintaining growth limitation due to availability of carbon source while avoiding total carbon source depletion. The use of this parameter, capable of maintaining the biomass in a physiological state of imminent but not actual exhaustion of carbon source, was successful in both the fed-batch and continuous production of heterologous laccase. Laccase production in fed-batch and continuous runs was considerably improved in comparison to the batch cultivation, both in terms of volumetric productivity and yield on lactose. The DELTABAS-control algorithm was shown to be a useful research tool for process optimization.
Original languageEnglish
Pages (from-to)484-491
JournalEnzyme and Microbial Technology
Volume41
Issue number4
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Laccase
Trichoderma
Carbon
Lactose
Fermenters
Fungi
Growth
Proteins
Biomass
Substrates
Productivity
Availability
Research

Keywords

  • Trichoderma reesei
  • Laccase production
  • Fed-batch
  • Continuous
  • Fermentation

Cite this

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title = "Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation",
abstract = "In this work, a thermostable laccase from the fungus Melanocarpus albomyces was produced in the efficient protein producer Trichoderma reesei in batch, fed-batch and continuous cultivations on lactose medium in a laboratory fermenter. The rate of consumption of base for pH control (DELTABAS) was measured in all the cultivations and was used as a control parameter in one fed-batch run and in a 1000 h continuous cultivation. The dynamic DELTABAS control parameter can be used to maintain optimal production of secreted protein by fitting the rate of substrate feeding to the amount of substrate available to the growing fungus in the fermenter, thus maintaining growth limitation due to availability of carbon source while avoiding total carbon source depletion. The use of this parameter, capable of maintaining the biomass in a physiological state of imminent but not actual exhaustion of carbon source, was successful in both the fed-batch and continuous production of heterologous laccase. Laccase production in fed-batch and continuous runs was considerably improved in comparison to the batch cultivation, both in terms of volumetric productivity and yield on lactose. The DELTABAS-control algorithm was shown to be a useful research tool for process optimization.",
keywords = "Trichoderma reesei, Laccase production, Fed-batch, Continuous, Fermentation",
author = "Michael Bailey and Bernhard Adamitsch and Jari Rautio and {von Weymarn}, Niklas and Markku Saloheimo",
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Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation. / Bailey, Michael (Corresponding Author); Adamitsch, Bernhard; Rautio, Jari; von Weymarn, Niklas; Saloheimo, Markku.

In: Enzyme and Microbial Technology, Vol. 41, No. 4, 2007, p. 484-491.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation

AU - Bailey, Michael

AU - Adamitsch, Bernhard

AU - Rautio, Jari

AU - von Weymarn, Niklas

AU - Saloheimo, Markku

PY - 2007

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AB - In this work, a thermostable laccase from the fungus Melanocarpus albomyces was produced in the efficient protein producer Trichoderma reesei in batch, fed-batch and continuous cultivations on lactose medium in a laboratory fermenter. The rate of consumption of base for pH control (DELTABAS) was measured in all the cultivations and was used as a control parameter in one fed-batch run and in a 1000 h continuous cultivation. The dynamic DELTABAS control parameter can be used to maintain optimal production of secreted protein by fitting the rate of substrate feeding to the amount of substrate available to the growing fungus in the fermenter, thus maintaining growth limitation due to availability of carbon source while avoiding total carbon source depletion. The use of this parameter, capable of maintaining the biomass in a physiological state of imminent but not actual exhaustion of carbon source, was successful in both the fed-batch and continuous production of heterologous laccase. Laccase production in fed-batch and continuous runs was considerably improved in comparison to the batch cultivation, both in terms of volumetric productivity and yield on lactose. The DELTABAS-control algorithm was shown to be a useful research tool for process optimization.

KW - Trichoderma reesei

KW - Laccase production

KW - Fed-batch

KW - Continuous

KW - Fermentation

U2 - 10.1016/j.enzmictec.2007.04.002

DO - 10.1016/j.enzmictec.2007.04.002

M3 - Article

VL - 41

SP - 484

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JO - Enzyme and Microbial Technology

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