Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela, Maija Liisa Suihko, Merja Penttilä, Sirkka Keränen

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalJournal of Biotechnology
Volume49
Issue number1-3
DOIs
Publication statusPublished - 20 Aug 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Diacetyl
Alcohol Dehydrogenase
Yeast
Brewing
Saccharomyces cerevisiae
acetolactate decarboxylase
Alcohols
Hypergravity
Bacterial Genes
Gene encoding
Gravitation
Oxidoreductases

Keywords

  • ADH1 Promoter
  • Brewer's yeast
  • Promoter regulation
  • α-Acetolactate decarboxylase

Cite this

Onnela, Maija Leena ; Suihko, Maija Liisa ; Penttilä, Merja ; Keränen, Sirkka. / Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast. In: Journal of Biotechnology. 1996 ; Vol. 49, No. 1-3. pp. 101-109.
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Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast. / Onnela, Maija Leena; Suihko, Maija Liisa; Penttilä, Merja; Keränen, Sirkka.

In: Journal of Biotechnology, Vol. 49, No. 1-3, 20.08.1996, p. 101-109.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Keränen, Sirkka

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