Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela, Maija Liisa Suihko, Merja Penttilä, Sirkka Keränen

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16 Citations (Scopus)

Abstract

The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalJournal of Biotechnology
Volume49
Issue number1-3
DOIs
Publication statusPublished - 20 Aug 1996
MoE publication typeA1 Journal article-refereed

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Keywords

  • ADH1 Promoter
  • Brewer's yeast
  • Promoter regulation
  • α-Acetolactate decarboxylase

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