Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela, Maija Liisa Suihko, Merja Penttilä, Sirkka Keränen*

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    18 Citations (Scopus)

    Abstract

    The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

    Original languageEnglish
    Pages (from-to)101-109
    Number of pages9
    JournalJournal of Biotechnology
    Volume49
    Issue number1-3
    DOIs
    Publication statusPublished - 20 Aug 1996
    MoE publication typeA1 Journal article-refereed

    Funding

    We thank the brewing technology section of VTT Biotechnology and Food Research for the analytical work and the technical assistance with the fermentations. This research was financed by Oy Panimolaboratorio-Bryggerilaboratorium Ab, Finland and by the Technology Development Centre (TEKES), Finland.

    Keywords

    • ADH1 Promoter
    • Brewer's yeast
    • Promoter regulation
    • α-Acetolactate decarboxylase

    Fingerprint

    Dive into the research topics of 'Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast'. Together they form a unique fingerprint.

    Cite this