Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela, Maija Liisa Suihko, Merja Penttilä, Sirkka Keränen

    Research output: Contribution to journalArticleScientificpeer-review

    17 Citations (Scopus)

    Abstract

    The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

    Original languageEnglish
    Pages (from-to)101-109
    Number of pages9
    JournalJournal of Biotechnology
    Volume49
    Issue number1-3
    DOIs
    Publication statusPublished - 20 Aug 1996
    MoE publication typeA1 Journal article-refereed

    Keywords

    • ADH1 Promoter
    • Brewer's yeast
    • Promoter regulation
    • α-Acetolactate decarboxylase

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