Abstract
The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.
Original language | English |
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Pages (from-to) | 101-109 |
Number of pages | 9 |
Journal | Journal of Biotechnology |
Volume | 49 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 20 Aug 1996 |
MoE publication type | A1 Journal article-refereed |
Funding
We thank the brewing technology section of VTT Biotechnology and Food Research for the analytical work and the technical assistance with the fermentations. This research was financed by Oy Panimolaboratorio-Bryggerilaboratorium Ab, Finland and by the Technology Development Centre (TEKES), Finland.
Keywords
- ADH1 Promoter
- Brewer's yeast
- Promoter regulation
- α-Acetolactate decarboxylase