Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela; Maija Liisa Suihko; Merja Penttilä; Sirkka Keränen

doi:10.1016/0168-1656(96)01532-5

Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija Leena Onnela, Maija Liisa Suihko, Merja Penttilä, Sirkka Keränen

Research output: Contribution to journal › Article › Scientific › peer-review

18 Citations (Scopus)

Abstract

The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

Original language	English
Pages (from-to)	101-109
Number of pages	9
Journal	Journal of Biotechnology
Volume	49
Issue number	1-3
DOIs	https://doi.org/10.1016/0168-1656(96)01532-5
Publication status	Published - 20 Aug 1996
MoE publication type	A1 Journal article-refereed

Keywords

ADH1 Promoter
Brewer's yeast
Promoter regulation
α-Acetolactate decarboxylase

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10.1016/0168-1656(96)01532-5

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abstract = "The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.",

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AU - Onnela, Maija Leena

AU - Suihko, Maija Liisa

AU - Penttilä, Merja

AU - Keränen, Sirkka

PY - 1996/8/20

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AB - The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of α-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

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KW - Brewer's yeast

KW - Promoter regulation

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ER -

Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Abstract

Keywords

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