Abstract
Air classification of an industrial barley endosperm fraction with initial protein content of 8.3% mixed with Aerosil 200F flow aid resulted in protein enrichment up to 28.3% with 21.7% protein separation efficiency. By reducing the classifier wheel speed from 21500 rpm to 8000 rpm, the protein separation efficiency increased to 59.4%, whereas the protein content only decreased to 22.3%. Microstructural analysis showed that small starch granules separated together with protein in the fine fraction and had more continuous protein matrix around them. The protein-enriched fraction obtained at 8000 rpm classifier wheel speed had limited protein solubility of 9.3% at native pH 5.1. Solubility increased in acidic (pH 3, 19.6%) and especially in alkaline (pH 11, 91.9%) conditions. Protein fraction had water and oil binding capacities of 1.2 and 1.1 g/g, respectively. Foaming properties tested at different pH-values revealed best capacity at pH 3. Thermograms showed two melting temperatures, varying from 60.0 to 64.2 °C and from 90.5 to 95.5 °C for all the studied fractions. Cereal side-streams such as the industrial barley endosperm fraction can be utilised as potential plant protein ingredient for various food/feed applications. However, further studies are needed to evaluate the technological, nutritional, and sensory characteristics of this protein enriched fraction.
| Original language | English |
|---|---|
| Pages (from-to) | 169-177 |
| Number of pages | 9 |
| Journal | Journal of Food Engineering |
| Volume | 222 |
| DOIs | |
| Publication status | Published - 1 Apr 2018 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Air classification
- Barley
- Flow aid
- Functional properties
- Protein
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Use of air classification technology to produce protein-enriched barley ingredients. / Silventoinen, Pia (Corresponding Author); Sipponen, Mika Henrikki; Holopainen-Mantila, Ulla; Poutanen, Kaisa; Sozer, Nesli.
In: Journal of Food Engineering, Vol. 222, 01.04.2018, p. 169-177.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Use of air classification technology to produce protein-enriched barley ingredients
AU - Silventoinen, Pia
AU - Sipponen, Mika Henrikki
AU - Holopainen-Mantila, Ulla
AU - Poutanen, Kaisa
AU - Sozer, Nesli
N1 - Project: 101795
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Air classification of an industrial barley endosperm fraction with initial protein content of 8.3% mixed with Aerosil 200F flow aid resulted in protein enrichment up to 28.3% with 21.7% protein separation efficiency. By reducing the classifier wheel speed from 21500 rpm to 8000 rpm, the protein separation efficiency increased to 59.4%, whereas the protein content only decreased to 22.3%. Microstructural analysis showed that small starch granules separated together with protein in the fine fraction and had more continuous protein matrix around them. The protein-enriched fraction obtained at 8000 rpm classifier wheel speed had limited protein solubility of 9.3% at native pH 5.1. Solubility increased in acidic (pH 3, 19.6%) and especially in alkaline (pH 11, 91.9%) conditions. Protein fraction had water and oil binding capacities of 1.2 and 1.1 g/g, respectively. Foaming properties tested at different pH-values revealed best capacity at pH 3. Thermograms showed two melting temperatures, varying from 60.0 to 64.2 °C and from 90.5 to 95.5 °C for all the studied fractions. Cereal side-streams such as the industrial barley endosperm fraction can be utilised as potential plant protein ingredient for various food/feed applications. However, further studies are needed to evaluate the technological, nutritional, and sensory characteristics of this protein enriched fraction.
AB - Air classification of an industrial barley endosperm fraction with initial protein content of 8.3% mixed with Aerosil 200F flow aid resulted in protein enrichment up to 28.3% with 21.7% protein separation efficiency. By reducing the classifier wheel speed from 21500 rpm to 8000 rpm, the protein separation efficiency increased to 59.4%, whereas the protein content only decreased to 22.3%. Microstructural analysis showed that small starch granules separated together with protein in the fine fraction and had more continuous protein matrix around them. The protein-enriched fraction obtained at 8000 rpm classifier wheel speed had limited protein solubility of 9.3% at native pH 5.1. Solubility increased in acidic (pH 3, 19.6%) and especially in alkaline (pH 11, 91.9%) conditions. Protein fraction had water and oil binding capacities of 1.2 and 1.1 g/g, respectively. Foaming properties tested at different pH-values revealed best capacity at pH 3. Thermograms showed two melting temperatures, varying from 60.0 to 64.2 °C and from 90.5 to 95.5 °C for all the studied fractions. Cereal side-streams such as the industrial barley endosperm fraction can be utilised as potential plant protein ingredient for various food/feed applications. However, further studies are needed to evaluate the technological, nutritional, and sensory characteristics of this protein enriched fraction.
KW - Air classification
KW - Barley
KW - Flow aid
KW - Functional properties
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=85034863097&partnerID=8YFLogxK
U2 - 10.1016/j.jfoodeng.2017.11.016
DO - 10.1016/j.jfoodeng.2017.11.016
M3 - Article
AN - SCOPUS:85034863097
VL - 222
SP - 169
EP - 177
JO - Journal of Food Engineering
JF - Journal of Food Engineering
SN - 0260-8774
ER -