Use of an extruder for pre-mixing enhances xylanase action on wheat bran at low water content

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8 Citations (Scopus)

Abstract

The aim of the work was to test the hypothesis that at low water content enzyme action on biomass is enhanced when the raw material is in the form of a continuous mass instead of powder/granular form. Effects of two pre-mixing methods, blade-mixing and extrusion, on xylanase action were studied during stationary incubation of wheat bran of different particle sizes, also in comparison with incubation at high water content with continuous stirring. The use of an extruder enhanced arabinoxylan (AX) solubilisation at low water content (<54%), as compared to blade-mixing. AX solubilisation was highest in the high-water stirring treatment, but based on molecular weight, xylanase action on solubilised AX was similar as in the extrusion-aided process. Pre-mixing by extrusion enabled efficient enzyme action at low water content without the requirement for continuous mixing, probably due to the enhanced diffusion by the formation of a continuous mass in the extruder.
Original languageEnglish
Pages (from-to)191-199
Number of pages8
JournalBioresource Technology
Volume149
DOIs
Publication statusPublished - 2013
MoE publication typeNot Eligible

Fingerprint

Extruders
Water content
wheat
water content
extrusion
Extrusion
solubilization
Enzymes
incubation
enzyme
Water treatment
Powders
water treatment
Raw materials
Biomass
Molecular weight
Particle size
particle size
Dietary Fiber
biomass

Keywords

  • enzymatic hydrolysis
  • high solids
  • mixing
  • particle size
  • wheat bran

Cite this

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title = "Use of an extruder for pre-mixing enhances xylanase action on wheat bran at low water content",
abstract = "The aim of the work was to test the hypothesis that at low water content enzyme action on biomass is enhanced when the raw material is in the form of a continuous mass instead of powder/granular form. Effects of two pre-mixing methods, blade-mixing and extrusion, on xylanase action were studied during stationary incubation of wheat bran of different particle sizes, also in comparison with incubation at high water content with continuous stirring. The use of an extruder enhanced arabinoxylan (AX) solubilisation at low water content (<54{\%}), as compared to blade-mixing. AX solubilisation was highest in the high-water stirring treatment, but based on molecular weight, xylanase action on solubilised AX was similar as in the extrusion-aided process. Pre-mixing by extrusion enabled efficient enzyme action at low water content without the requirement for continuous mixing, probably due to the enhanced diffusion by the formation of a continuous mass in the extruder.",
keywords = "enzymatic hydrolysis, high solids, mixing, particle size, wheat bran",
author = "Outi Santala and Emilia Nordlund and Kaisa Poutanen",
year = "2013",
doi = "10.1016/j.biortech.2013.09.029",
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journal = "Bioresource Technology",
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T1 - Use of an extruder for pre-mixing enhances xylanase action on wheat bran at low water content

AU - Santala, Outi

AU - Nordlund, Emilia

AU - Poutanen, Kaisa

PY - 2013

Y1 - 2013

N2 - The aim of the work was to test the hypothesis that at low water content enzyme action on biomass is enhanced when the raw material is in the form of a continuous mass instead of powder/granular form. Effects of two pre-mixing methods, blade-mixing and extrusion, on xylanase action were studied during stationary incubation of wheat bran of different particle sizes, also in comparison with incubation at high water content with continuous stirring. The use of an extruder enhanced arabinoxylan (AX) solubilisation at low water content (<54%), as compared to blade-mixing. AX solubilisation was highest in the high-water stirring treatment, but based on molecular weight, xylanase action on solubilised AX was similar as in the extrusion-aided process. Pre-mixing by extrusion enabled efficient enzyme action at low water content without the requirement for continuous mixing, probably due to the enhanced diffusion by the formation of a continuous mass in the extruder.

AB - The aim of the work was to test the hypothesis that at low water content enzyme action on biomass is enhanced when the raw material is in the form of a continuous mass instead of powder/granular form. Effects of two pre-mixing methods, blade-mixing and extrusion, on xylanase action were studied during stationary incubation of wheat bran of different particle sizes, also in comparison with incubation at high water content with continuous stirring. The use of an extruder enhanced arabinoxylan (AX) solubilisation at low water content (<54%), as compared to blade-mixing. AX solubilisation was highest in the high-water stirring treatment, but based on molecular weight, xylanase action on solubilised AX was similar as in the extrusion-aided process. Pre-mixing by extrusion enabled efficient enzyme action at low water content without the requirement for continuous mixing, probably due to the enhanced diffusion by the formation of a continuous mass in the extruder.

KW - enzymatic hydrolysis

KW - high solids

KW - mixing

KW - particle size

KW - wheat bran

U2 - 10.1016/j.biortech.2013.09.029

DO - 10.1016/j.biortech.2013.09.029

M3 - Article

VL - 149

SP - 191

EP - 199

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

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