Use of brewer's yeast expressing alpha-acetolactate decarboxylase in conventional and immobilized fermentations

Matti Linko, Maija-Liisa Suihko, Jukka Kronlöf, Silja Home

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Fermentation trials using four recombinant brewers' yeast strains, transformed using the alpha acetolactate decarboxylase gene from either Klebsiella terrigena or Enterobacter aerogenes with either the ADH1 or PGK1 promoter in order to reduce the level of diacetyl formation, in both a conventional batch process and a continuous process in which the yeast was immobilized on porous glass beads, are described. All the transformed yeast strains performed well, producing beers of similar composition and flavour to those produced by the untransformed source strain. Diacetyl production was much lower than in the control fermentations, especially in the two yeasts in which the gene was expressed using the PGK1 promoter, allowing the maturation period to be much shortened or even eliminated.
Original languageEnglish
Pages (from-to)93 - 97
Number of pages5
JournalMBAA Technical Quarterly
Volume30
Issue number3
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

Fingerprint

acetolactate decarboxylase
Fermentation
Enterobacter aerogenes
Saccharomyces cerevisiae
Diacetyl
Yeasts
Genes
Glass

Keywords

  • accelerated brewers' yeast diacetyl fermentation inhibition transformation yeast strain

Cite this

Linko, Matti ; Suihko, Maija-Liisa ; Kronlöf, Jukka ; Home, Silja. / Use of brewer's yeast expressing alpha-acetolactate decarboxylase in conventional and immobilized fermentations. In: MBAA Technical Quarterly. 1993 ; Vol. 30, No. 3. pp. 93 - 97.
@article{c2584610f58f4ccfa2b2156bfe5b271e,
title = "Use of brewer's yeast expressing alpha-acetolactate decarboxylase in conventional and immobilized fermentations",
abstract = "Fermentation trials using four recombinant brewers' yeast strains, transformed using the alpha acetolactate decarboxylase gene from either Klebsiella terrigena or Enterobacter aerogenes with either the ADH1 or PGK1 promoter in order to reduce the level of diacetyl formation, in both a conventional batch process and a continuous process in which the yeast was immobilized on porous glass beads, are described. All the transformed yeast strains performed well, producing beers of similar composition and flavour to those produced by the untransformed source strain. Diacetyl production was much lower than in the control fermentations, especially in the two yeasts in which the gene was expressed using the PGK1 promoter, allowing the maturation period to be much shortened or even eliminated.",
keywords = "accelerated brewers' yeast diacetyl fermentation inhibition transformation yeast strain",
author = "Matti Linko and Maija-Liisa Suihko and Jukka Kronl{\"o}f and Silja Home",
note = "Project code: BIO1027",
year = "1993",
language = "English",
volume = "30",
pages = "93 -- 97",
journal = "MBAA Technical Quarterly",
issn = "0743-9407",
number = "3",

}

Use of brewer's yeast expressing alpha-acetolactate decarboxylase in conventional and immobilized fermentations. / Linko, Matti; Suihko, Maija-Liisa; Kronlöf, Jukka; Home, Silja.

In: MBAA Technical Quarterly, Vol. 30, No. 3, 1993, p. 93 - 97.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Use of brewer's yeast expressing alpha-acetolactate decarboxylase in conventional and immobilized fermentations

AU - Linko, Matti

AU - Suihko, Maija-Liisa

AU - Kronlöf, Jukka

AU - Home, Silja

N1 - Project code: BIO1027

PY - 1993

Y1 - 1993

N2 - Fermentation trials using four recombinant brewers' yeast strains, transformed using the alpha acetolactate decarboxylase gene from either Klebsiella terrigena or Enterobacter aerogenes with either the ADH1 or PGK1 promoter in order to reduce the level of diacetyl formation, in both a conventional batch process and a continuous process in which the yeast was immobilized on porous glass beads, are described. All the transformed yeast strains performed well, producing beers of similar composition and flavour to those produced by the untransformed source strain. Diacetyl production was much lower than in the control fermentations, especially in the two yeasts in which the gene was expressed using the PGK1 promoter, allowing the maturation period to be much shortened or even eliminated.

AB - Fermentation trials using four recombinant brewers' yeast strains, transformed using the alpha acetolactate decarboxylase gene from either Klebsiella terrigena or Enterobacter aerogenes with either the ADH1 or PGK1 promoter in order to reduce the level of diacetyl formation, in both a conventional batch process and a continuous process in which the yeast was immobilized on porous glass beads, are described. All the transformed yeast strains performed well, producing beers of similar composition and flavour to those produced by the untransformed source strain. Diacetyl production was much lower than in the control fermentations, especially in the two yeasts in which the gene was expressed using the PGK1 promoter, allowing the maturation period to be much shortened or even eliminated.

KW - accelerated brewers' yeast diacetyl fermentation inhibition transformation yeast strain

M3 - Article

VL - 30

SP - 93

EP - 97

JO - MBAA Technical Quarterly

JF - MBAA Technical Quarterly

SN - 0743-9407

IS - 3

ER -