Use of evaporative light scattering mass detection in high performance liquid chromatography of triterpenes in the bark resin of Betula species

Hannu Taipale, Seppo Lapinjoki (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Plants often contain triterpenes that are difficult to analyse by high performance liquid chromatography (HPLC) due to lack of double bonds or other chromophores that can be readily detected spectrophotometrically. The present results show that light scattering mass detection (LS) is useful in such cases. We have been able to replace thin layer chromatography (TLC) with HPLC by using LS in evaluating triterpene contents in the bark resin of different birches (Betula pendula Roth., B. resinifera (Regel) Fern. & Raup., B. platyphylla Sucachev, B. ermanii Cham., B. papyrifera Marsh and B. pubescens Ehrh.). LS was five‐fold more sensitive than UV for papyriferic acid, which was used as a reference compound, and HPLC/LS revealed new compounds and qualitative relations that were undetectable by UV or TLC. This paper describes detection of a new major triterpenoid from B. pendula, the age dependency of the triterpenoid content in B. pendula and B. papyrifera and gives an interspecies comparison of the triterpenoid content of the different species.

Original languageEnglish
Pages (from-to)84 - 86
Number of pages3
JournalPhytochemical Analysis
Volume2
Issue number2
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Betula
Triterpenes
High performance liquid chromatography
light scattering
triterpenoids
Light scattering
resins
Thin layer chromatography
bark
Resins
high performance liquid chromatography
High Pressure Liquid Chromatography
Thin Layer Chromatography
Betula pendula
Light
Ferns
thin layer chromatography
Wetlands
Chromophores
Betula ermanii

Cite this

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title = "Use of evaporative light scattering mass detection in high performance liquid chromatography of triterpenes in the bark resin of Betula species",
abstract = "Plants often contain triterpenes that are difficult to analyse by high performance liquid chromatography (HPLC) due to lack of double bonds or other chromophores that can be readily detected spectrophotometrically. The present results show that light scattering mass detection (LS) is useful in such cases. We have been able to replace thin layer chromatography (TLC) with HPLC by using LS in evaluating triterpene contents in the bark resin of different birches (Betula pendula Roth., B. resinifera (Regel) Fern. & Raup., B. platyphylla Sucachev, B. ermanii Cham., B. papyrifera Marsh and B. pubescens Ehrh.). LS was five‐fold more sensitive than UV for papyriferic acid, which was used as a reference compound, and HPLC/LS revealed new compounds and qualitative relations that were undetectable by UV or TLC. This paper describes detection of a new major triterpenoid from B. pendula, the age dependency of the triterpenoid content in B. pendula and B. papyrifera and gives an interspecies comparison of the triterpenoid content of the different species.",
author = "Hannu Taipale and Seppo Lapinjoki",
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year = "1991",
doi = "10.1002/pca.2800020209",
language = "English",
volume = "2",
pages = "84 -- 86",
journal = "Phytochemical Analysis",
issn = "0958-0344",
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}

Use of evaporative light scattering mass detection in high performance liquid chromatography of triterpenes in the bark resin of Betula species. / Taipale, Hannu; Lapinjoki, Seppo (Corresponding Author).

In: Phytochemical Analysis, Vol. 2, No. 2, 1991, p. 84 - 86.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Use of evaporative light scattering mass detection in high performance liquid chromatography of triterpenes in the bark resin of Betula species

AU - Taipale, Hannu

AU - Lapinjoki, Seppo

N1 - Project code: BIO9001

PY - 1991

Y1 - 1991

N2 - Plants often contain triterpenes that are difficult to analyse by high performance liquid chromatography (HPLC) due to lack of double bonds or other chromophores that can be readily detected spectrophotometrically. The present results show that light scattering mass detection (LS) is useful in such cases. We have been able to replace thin layer chromatography (TLC) with HPLC by using LS in evaluating triterpene contents in the bark resin of different birches (Betula pendula Roth., B. resinifera (Regel) Fern. & Raup., B. platyphylla Sucachev, B. ermanii Cham., B. papyrifera Marsh and B. pubescens Ehrh.). LS was five‐fold more sensitive than UV for papyriferic acid, which was used as a reference compound, and HPLC/LS revealed new compounds and qualitative relations that were undetectable by UV or TLC. This paper describes detection of a new major triterpenoid from B. pendula, the age dependency of the triterpenoid content in B. pendula and B. papyrifera and gives an interspecies comparison of the triterpenoid content of the different species.

AB - Plants often contain triterpenes that are difficult to analyse by high performance liquid chromatography (HPLC) due to lack of double bonds or other chromophores that can be readily detected spectrophotometrically. The present results show that light scattering mass detection (LS) is useful in such cases. We have been able to replace thin layer chromatography (TLC) with HPLC by using LS in evaluating triterpene contents in the bark resin of different birches (Betula pendula Roth., B. resinifera (Regel) Fern. & Raup., B. platyphylla Sucachev, B. ermanii Cham., B. papyrifera Marsh and B. pubescens Ehrh.). LS was five‐fold more sensitive than UV for papyriferic acid, which was used as a reference compound, and HPLC/LS revealed new compounds and qualitative relations that were undetectable by UV or TLC. This paper describes detection of a new major triterpenoid from B. pendula, the age dependency of the triterpenoid content in B. pendula and B. papyrifera and gives an interspecies comparison of the triterpenoid content of the different species.

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DO - 10.1002/pca.2800020209

M3 - Article

VL - 2

SP - 84

EP - 86

JO - Phytochemical Analysis

JF - Phytochemical Analysis

SN - 0958-0344

IS - 2

ER -