Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

Marja-Leena Laukkanen (Corresponding Author), Adelina Orellana, Kari Keinänen

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27 Citations (Scopus)


Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes.
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
Original languageEnglish
Pages (from-to)95-102
JournalJournal of Immunological Methods
Issue number1
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed


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