Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

Marja-Leena Laukkanen (Corresponding Author), Adelina Orellana, Kari Keinänen

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)

Abstract

Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes.
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
Original languageEnglish
Pages (from-to)95-102
JournalJournal of Immunological Methods
Volume185
Issue number1
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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Fluoroimmunoassay
Europium
Single-Chain Antibodies
Liposomes
Lipids
Antibodies
Haptens
Immunoassay
Biochemistry
Proteins

Cite this

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title = "Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay",
abstract = "Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.",
author = "Marja-Leena Laukkanen and Adelina Orellana and Kari Kein{\"a}nen",
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Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay. / Laukkanen, Marja-Leena (Corresponding Author); Orellana, Adelina; Keinänen, Kari.

In: Journal of Immunological Methods, Vol. 185, No. 1, 1995, p. 95-102.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

AU - Laukkanen, Marja-Leena

AU - Orellana, Adelina

AU - Keinänen, Kari

N1 - Project code: BEL2031

PY - 1995

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AB - Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.

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DO - 10.1016/0022-1759(95)00107-L

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