Abstract
Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes.
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
| Original language | English |
|---|---|
| Pages (from-to) | 95-102 |
| Journal | Journal of Immunological Methods |
| Volume | 185 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1995 |
| MoE publication type | A1 Journal article-refereed |
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Laukkanen, M-L., Orellana, A., & Keinänen, K. (1995). Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay. Journal of Immunological Methods, 185(1), 95-102. https://doi.org/10.1016/0022-1759(95)00107-L