Abstract
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
| Original language | English |
|---|---|
| Pages (from-to) | 95-102 |
| Journal | Journal of Immunological Methods |
| Volume | 185 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1995 |
| MoE publication type | A1 Journal article-refereed |
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Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay. / Laukkanen, Marja-Leena (Corresponding Author); Orellana, Adelina; Keinänen, Kari.
In: Journal of Immunological Methods, Vol. 185, No. 1, 1995, p. 95-102.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay
AU - Laukkanen, Marja-Leena
AU - Orellana, Adelina
AU - Keinänen, Kari
N1 - Project code: BEL2031
PY - 1995
Y1 - 1995
N2 - Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
AB - Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
U2 - 10.1016/0022-1759(95)00107-L
DO - 10.1016/0022-1759(95)00107-L
M3 - Article
VL - 185
SP - 95
EP - 102
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -