Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

Marja-Leena Laukkanen; Adelina Orellana; Kari Keinänen

doi:10.1016/0022-1759(95)00107-L

Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

Marja-Leena Laukkanen (Corresponding Author), Adelina Orellana, Kari Keinänen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

29 Citations (Scopus)

Abstract

Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes.
The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay.
These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.

Original language	English
Pages (from-to)	95-102
Journal	Journal of Immunological Methods
Volume	185
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(95)00107-L
Publication status	Published - 1995
MoE publication type	A1 Journal article-refereed

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10.1016/0022-1759(95)00107-L

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title = "Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay",

abstract = "Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.",

author = "Marja-Leena Laukkanen and Adelina Orellana and Kari Kein{\"a}nen",

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Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay. / Laukkanen, Marja-Leena (Corresponding Author); Orellana, Adelina; Keinänen, Kari.

In: Journal of Immunological Methods, Vol. 185, No. 1, 1995, p. 95-102.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

AU - Laukkanen, Marja-Leena

AU - Orellana, Adelina

AU - Keinänen, Kari

N1 - Project code: BEL2031

PY - 1995

Y1 - 1995

N2 - Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.

AB - Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4°C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.

U2 - 10.1016/0022-1759(95)00107-L

DO - 10.1016/0022-1759(95)00107-L

M3 - Article

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

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ER -

Use of genetically engineered lipid-tagged antibody to generate functional europium chelate-loaded liposomes. Application in fluoroimmunoassay

Abstract

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