Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

K. Yun, Eiry Kobatake, Tetsuya Haruyama, Marja-Leena Laukkanen, Kari Keinänen, Masuo Aizawa (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes.
The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.
Original languageEnglish
Pages (from-to)260-264
JournalAnalytical Chemistry
Volume70
Issue number2
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Haptens
Quartz crystal microbalances
Liposomes
Antigens
Single-Chain Antibodies
Crystals
Phosphatidylcholines
Assays
Lipids
2-phenyloxazolone

Cite this

Yun, K., Kobatake, E., Haruyama, T., Laukkanen, M-L., Keinänen, K., & Aizawa, M. (1998). Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction. Analytical Chemistry, 70(2), 260-264. https://doi.org/10.1021/ac970234+
Yun, K. ; Kobatake, Eiry ; Haruyama, Tetsuya ; Laukkanen, Marja-Leena ; Keinänen, Kari ; Aizawa, Masuo. / Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction. In: Analytical Chemistry. 1998 ; Vol. 70, No. 2. pp. 260-264.
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Yun, K, Kobatake, E, Haruyama, T, Laukkanen, M-L, Keinänen, K & Aizawa, M 1998, 'Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction', Analytical Chemistry, vol. 70, no. 2, pp. 260-264. https://doi.org/10.1021/ac970234+

Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction. / Yun, K.; Kobatake, Eiry; Haruyama, Tetsuya; Laukkanen, Marja-Leena; Keinänen, Kari; Aizawa, Masuo (Corresponding Author).

In: Analytical Chemistry, Vol. 70, No. 2, 1998, p. 260-264.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Yun, K.

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AB - We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.

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Yun K, Kobatake E, Haruyama T, Laukkanen M-L, Keinänen K, Aizawa M. Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction. Analytical Chemistry. 1998;70(2):260-264. https://doi.org/10.1021/ac970234+