Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

K. Yun, Eiry Kobatake, Tetsuya Haruyama, Marja-Leena Laukkanen, Kari Keinänen, Masuo Aizawa (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

52 Citations (Scopus)


We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes.
The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.
Original languageEnglish
Pages (from-to)260-264
JournalAnalytical Chemistry
Issue number2
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed


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