Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

K. Yun; Eiry Kobatake; Tetsuya Haruyama; Marja-Leena Laukkanen; Kari Keinänen; Masuo Aizawa

doi:10.1021/ac970234+

Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

K. Yun
, Eiry Kobatake
, Tetsuya Haruyama
, Marja-Leena Laukkanen
, Kari Keinänen
, Masuo Aizawa^*

^*Corresponding author for this work

VTT Technical Research Centre of Finland

Tokyo Institute of Technology
VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

53 Citations (Scopus)

Abstract

We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes.
The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.

Original language	English
Pages (from-to)	260-264
Journal	Analytical Chemistry
Volume	70
Issue number	2
DOIs	https://doi.org/10.1021/ac970234+
Publication status	Published - 1998
MoE publication type	A1 Journal article-refereed

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title = "Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction",

abstract = "We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.",

author = "K. Yun and Eiry Kobatake and Tetsuya Haruyama and Marja-Leena Laukkanen and Kari Kein{\"a}nen and Masuo Aizawa",

year = "1998",

doi = "10.1021/ac970234+",

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pages = "260--264",

journal = "Analytical Chemistry",

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T1 - Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

AU - Yun, K.

AU - Kobatake, Eiry

AU - Haruyama, Tetsuya

AU - Laukkanen, Marja-Leena

AU - Keinänen, Kari

AU - Aizawa, Masuo

PY - 1998

Y1 - 1998

N2 - We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.

AB - We have used quartz crystal microbalance (QCM)-based real-time biospecific interaction measurement to analyze the binding of immunoliposomes to antigen and examined the use of liposomes as signal-enhancing reagents in competitive QCM immunoassay. For the preparation of immunoliposomes, various amounts of bacterially produced lipid-tagged single-chain antibody against 2-phenyloxazolone were incorporated in phosphatidylcholine liposomes. The immunoliposomes bound specifically to immobilized hapten, and this binding was inhibited by soluble hapten in a concentration-dependent manner. In this competitive assay, antigen could be measured in the concentration range from 10-5 to 10-8 M.

U2 - 10.1021/ac970234+

DO - 10.1021/ac970234+

M3 - Article

SN - 0003-2700

VL - 70

SP - 260

EP - 264

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -

Use of quarz crystal microbalance to monitor immunoliposome-antigen interaction

Abstract

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