Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs

Kaarina Aarnisalo (Corresponding Author), Elina Vihavainen, Leila Rantala, Riitta Maijala, Maija-Liisa Suihko, Sebastian Hielm, Pirkko Tuominen, Jukka Ranta, Laura Raaska

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.
Original languageEnglish
Pages (from-to)275 - 284
Number of pages10
JournalInternational Journal of Food Microbiology
Volume121
Issue number3
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Listeria monocytogenes
Leg
legs
broiler chickens
Finland
risk assessment
microbiological risk assessment
Listeriosis
listeriosis
probability distribution
control methods
public health
foods
Software
Public Health
Genotype
Food
genotype

Keywords

  • risk assessment
  • processing plants
  • Listeria monocytogenes
  • broiler legs
  • listeriosis

Cite this

Aarnisalo, Kaarina ; Vihavainen, Elina ; Rantala, Leila ; Maijala, Riitta ; Suihko, Maija-Liisa ; Hielm, Sebastian ; Tuominen, Pirkko ; Ranta, Jukka ; Raaska, Laura. / Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs. In: International Journal of Food Microbiology. 2008 ; Vol. 121, No. 3. pp. 275 - 284.
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abstract = "Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90{\%} of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS{\circledR}, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95{\%} credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34{\%} ± 1{\%} of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95{\%} CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.",
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Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs. / Aarnisalo, Kaarina (Corresponding Author); Vihavainen, Elina; Rantala, Leila; Maijala, Riitta; Suihko, Maija-Liisa; Hielm, Sebastian; Tuominen, Pirkko; Ranta, Jukka; Raaska, Laura.

In: International Journal of Food Microbiology, Vol. 121, No. 3, 2008, p. 275 - 284.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs

AU - Aarnisalo, Kaarina

AU - Vihavainen, Elina

AU - Rantala, Leila

AU - Maijala, Riitta

AU - Suihko, Maija-Liisa

AU - Hielm, Sebastian

AU - Tuominen, Pirkko

AU - Ranta, Jukka

AU - Raaska, Laura

PY - 2008

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N2 - Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.

AB - Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.

KW - risk assessment

KW - processing plants

KW - Listeria monocytogenes

KW - broiler legs

KW - listeriosis

U2 - 10.1016/j.ijfoodmicro.2007.11.037

DO - 10.1016/j.ijfoodmicro.2007.11.037

M3 - Article

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JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

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ER -