Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs

Kaarina Aarnisalo (Corresponding Author), Elina Vihavainen, Leila Rantala, Riitta Maijala, Maija-Liisa Suihko, Sebastian Hielm, Pirkko Tuominen, Jukka Ranta, Laura Raaska

    Research output: Contribution to journalArticleScientificpeer-review

    9 Citations (Scopus)

    Abstract

    Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.
    Original languageEnglish
    Pages (from-to)275 - 284
    Number of pages10
    JournalInternational Journal of Food Microbiology
    Volume121
    Issue number3
    DOIs
    Publication statusPublished - 2008
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Listeria monocytogenes
    Leg
    legs
    broiler chickens
    Finland
    risk assessment
    microbiological risk assessment
    Listeriosis
    listeriosis
    probability distribution
    control methods
    public health
    foods
    Software
    Public Health
    Genotype
    Food
    genotype

    Keywords

    • risk assessment
    • processing plants
    • Listeria monocytogenes
    • broiler legs
    • listeriosis

    Cite this

    Aarnisalo, Kaarina ; Vihavainen, Elina ; Rantala, Leila ; Maijala, Riitta ; Suihko, Maija-Liisa ; Hielm, Sebastian ; Tuominen, Pirkko ; Ranta, Jukka ; Raaska, Laura. / Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs. In: International Journal of Food Microbiology. 2008 ; Vol. 121, No. 3. pp. 275 - 284.
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    abstract = "Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90{\%} of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS{\circledR}, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95{\%} credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34{\%} ± 1{\%} of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95{\%} CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.",
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    author = "Kaarina Aarnisalo and Elina Vihavainen and Leila Rantala and Riitta Maijala and Maija-Liisa Suihko and Sebastian Hielm and Pirkko Tuominen and Jukka Ranta and Laura Raaska",
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    Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs. / Aarnisalo, Kaarina (Corresponding Author); Vihavainen, Elina; Rantala, Leila; Maijala, Riitta; Suihko, Maija-Liisa; Hielm, Sebastian; Tuominen, Pirkko; Ranta, Jukka; Raaska, Laura.

    In: International Journal of Food Microbiology, Vol. 121, No. 3, 2008, p. 275 - 284.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Use of results of microbiological analyses for risk-based control of Listeria monocytogenes in marinated broiler legs

    AU - Aarnisalo, Kaarina

    AU - Vihavainen, Elina

    AU - Rantala, Leila

    AU - Maijala, Riitta

    AU - Suihko, Maija-Liisa

    AU - Hielm, Sebastian

    AU - Tuominen, Pirkko

    AU - Ranta, Jukka

    AU - Raaska, Laura

    PY - 2008

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    N2 - Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.

    AB - Microbial risk assessment provides a means of estimating consumer risks associated with food products. The methods can also be applied at the plant level. In this study results of microbiological analyses were used to develop a robust single plant level risk assessment. Furthermore, the prevalence and numbers of Listeria monocytogenes in marinated broiler legs in Finland were estimated. These estimates were based on information on the prevalence, numbers and genotypes of L. monocytogenes in 186 marinated broiler legs from 41 retail stores. The products were from three main Finnish producers, which produce 90% of all marinated broiler legs sold in Finland. The prevalence and numbers of L. monocytogenes were estimated by Monte Carlo simulation using WinBUGS®, but the model is applicable to any software featuring standard probability distributions. The estimated mean annual number of L. monocytogenes-positive broiler legs sold in Finland was 7.2 × 106 with a 95% credible interval (CI) 6.7 × 106–7.7 × 106. That would be 34% ± 1% of the marinated broiler legs sold in Finland. The mean number of L. monocytogenes in marinated broiler legs estimated at the sell-by-date was 2 CFU/g, with a 95% CI of 0–14 CFU/g. Producer-specific L. monocytogenes strains were recovered from the products throughout the year, which emphasizes the importance of characterizing the isolates and identifying strains that may cause problems as part of risk assessment studies. As the levels of L. monocytogenes were low, the risk of acquiring listeriosis from these products proved to be insignificant. Consequently there was no need for a thorough national level risk assessment. However, an approach using worst-case and average point estimates was applied to produce an example of single producer level risk assessment based on limited data. This assessment also indicated that the risk from these products was low. The risk-based approach presented in this work can provide estimation of public health risk on which control measures at the plant level can be based.

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    KW - processing plants

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    KW - listeriosis

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