Utilization of glucoisosaccharinic acid by a bacterial isolate unable to metabolize glucose

Michael Bailey

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Enrichment cultivation was used to select a bacterial strain capable of metabolizing glucoisosaccharinic acid (GISA), the major organic acid component of black liquor from the sulphate cellulose (Kraft) process. The bacterium was isolated from mud at the bottom of a lake polluted by pulping effluents. It grew well on GISA as the sole carbon source at pH 6.0±0.5, the absolute limits for growth on this substrate being 5.1<pH<7.2. It was, however, unable to metabolize glucose under any of the conditions tested. Glucoisosaccharinic acid at an initial concentration of 2 g l-1 was consumed within 24 h in batch laboratory fermentations, the maximum observed rate of consumption after the lag phase being almost 10 g l-1 d-1. The strain appeared to be independent of organic nitrogen.

Original languageEnglish
Pages (from-to)493 - 498
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume24
Issue number6
DOIs
Publication statusPublished - 1986
MoE publication typeNot Eligible

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Glucose
Acids
Lakes
Fermentation
Nitrogen
Carbon
Bacteria
Growth
cellulose sulfate

Cite this

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title = "Utilization of glucoisosaccharinic acid by a bacterial isolate unable to metabolize glucose",
abstract = "Enrichment cultivation was used to select a bacterial strain capable of metabolizing glucoisosaccharinic acid (GISA), the major organic acid component of black liquor from the sulphate cellulose (Kraft) process. The bacterium was isolated from mud at the bottom of a lake polluted by pulping effluents. It grew well on GISA as the sole carbon source at pH 6.0±0.5, the absolute limits for growth on this substrate being 5.1<pH<7.2. It was, however, unable to metabolize glucose under any of the conditions tested. Glucoisosaccharinic acid at an initial concentration of 2 g l-1 was consumed within 24 h in batch laboratory fermentations, the maximum observed rate of consumption after the lag phase being almost 10 g l-1 d-1. The strain appeared to be independent of organic nitrogen.",
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Utilization of glucoisosaccharinic acid by a bacterial isolate unable to metabolize glucose. / Bailey, Michael.

In: Applied Microbiology and Biotechnology, Vol. 24, No. 6, 1986, p. 493 - 498.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Utilization of glucoisosaccharinic acid by a bacterial isolate unable to metabolize glucose

AU - Bailey, Michael

PY - 1986

Y1 - 1986

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AB - Enrichment cultivation was used to select a bacterial strain capable of metabolizing glucoisosaccharinic acid (GISA), the major organic acid component of black liquor from the sulphate cellulose (Kraft) process. The bacterium was isolated from mud at the bottom of a lake polluted by pulping effluents. It grew well on GISA as the sole carbon source at pH 6.0±0.5, the absolute limits for growth on this substrate being 5.1<pH<7.2. It was, however, unable to metabolize glucose under any of the conditions tested. Glucoisosaccharinic acid at an initial concentration of 2 g l-1 was consumed within 24 h in batch laboratory fermentations, the maximum observed rate of consumption after the lag phase being almost 10 g l-1 d-1. The strain appeared to be independent of organic nitrogen.

U2 - 10.1007/BF00250329

DO - 10.1007/BF00250329

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VL - 24

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JO - Applied Microbiology and Biotechnology

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