UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers

Tero Kämäräinen, Lokanathan R. Arcot (Corresponding Author), Leena-Sisko Johansson, Joseph Campbell, Tekla Tammelin, Sami Franssila, Janne Laine, Orlando, J. Rojas

    Research output: Contribution to journalArticleScientificpeer-review

    6 Citations (Scopus)

    Abstract

    We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.
    Original languageEnglish
    Pages (from-to)1847-1857
    JournalCellulose
    Volume23
    Issue number3
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Cellulose films
    Ozone
    Self assembled monolayers
    Cellulose
    Bovine Serum Albumin
    Adsorption
    Wetting
    Proteins
    Photolithography
    Interfacial energy
    Microfluidics
    Contact angle
    Optical microscopy
    Surface morphology
    Masks
    Monolayers
    Atomic force microscopy
    Coloring Agents
    Visualization
    X ray photoelectron spectroscopy

    Keywords

    • nanocellulose (MNFC)
    • photolithography
    • protein patterning
    • self-assembled monolayer (SAM)
    • silane

    Cite this

    Kämäräinen, T., Arcot, L. R., Johansson, L-S., Campbell, J., Tammelin, T., Franssila, S., ... Rojas, O. J. (2016). UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. Cellulose, 23(3), 1847-1857. https://doi.org/10.1007/s10570-016-0942-x
    Kämäräinen, Tero ; Arcot, Lokanathan R. ; Johansson, Leena-Sisko ; Campbell, Joseph ; Tammelin, Tekla ; Franssila, Sami ; Laine, Janne ; Rojas, Orlando, J. / UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. In: Cellulose. 2016 ; Vol. 23, No. 3. pp. 1847-1857.
    @article{c654ad87c7584fc88bb3a7b74764a081,
    title = "UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers",
    abstract = "We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.",
    keywords = "nanocellulose (MNFC), photolithography, protein patterning, self-assembled monolayer (SAM), silane",
    author = "Tero K{\"a}m{\"a}r{\"a}inen and Arcot, {Lokanathan R.} and Leena-Sisko Johansson and Joseph Campbell and Tekla Tammelin and Sami Franssila and Janne Laine and Rojas, {Orlando, J.}",
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    Kämäräinen, T, Arcot, LR, Johansson, L-S, Campbell, J, Tammelin, T, Franssila, S, Laine, J & Rojas, OJ 2016, 'UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers', Cellulose, vol. 23, no. 3, pp. 1847-1857. https://doi.org/10.1007/s10570-016-0942-x

    UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. / Kämäräinen, Tero; Arcot, Lokanathan R. (Corresponding Author); Johansson, Leena-Sisko; Campbell, Joseph; Tammelin, Tekla; Franssila, Sami; Laine, Janne; Rojas, Orlando, J.

    In: Cellulose, Vol. 23, No. 3, 2016, p. 1847-1857.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers

    AU - Kämäräinen, Tero

    AU - Arcot, Lokanathan R.

    AU - Johansson, Leena-Sisko

    AU - Campbell, Joseph

    AU - Tammelin, Tekla

    AU - Franssila, Sami

    AU - Laine, Janne

    AU - Rojas, Orlando, J.

    PY - 2016

    Y1 - 2016

    N2 - We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.

    AB - We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.

    KW - nanocellulose (MNFC)

    KW - photolithography

    KW - protein patterning

    KW - self-assembled monolayer (SAM)

    KW - silane

    U2 - 10.1007/s10570-016-0942-x

    DO - 10.1007/s10570-016-0942-x

    M3 - Article

    VL - 23

    SP - 1847

    EP - 1857

    JO - Cellulose

    JF - Cellulose

    SN - 0969-0239

    IS - 3

    ER -