UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers

Tero Kämäräinen, Lokanathan R. Arcot (Corresponding Author), Leena-Sisko Johansson, Joseph Campbell, Tekla Tammelin, Sami Franssila, Janne Laine, Orlando, J. Rojas

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.
Original languageEnglish
Pages (from-to)1847-1857
JournalCellulose
Volume23
Issue number3
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose films
Ozone
Self assembled monolayers
Cellulose
Bovine Serum Albumin
Adsorption
Wetting
Proteins
Photolithography
Interfacial energy
Microfluidics
Contact angle
Optical microscopy
Surface morphology
Masks
Monolayers
Atomic force microscopy
Coloring Agents
Visualization
X ray photoelectron spectroscopy

Keywords

  • nanocellulose (MNFC)
  • photolithography
  • protein patterning
  • self-assembled monolayer (SAM)
  • silane

Cite this

Kämäräinen, T., Arcot, L. R., Johansson, L-S., Campbell, J., Tammelin, T., Franssila, S., ... Rojas, O. J. (2016). UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. Cellulose, 23(3), 1847-1857. https://doi.org/10.1007/s10570-016-0942-x
Kämäräinen, Tero ; Arcot, Lokanathan R. ; Johansson, Leena-Sisko ; Campbell, Joseph ; Tammelin, Tekla ; Franssila, Sami ; Laine, Janne ; Rojas, Orlando, J. / UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. In: Cellulose. 2016 ; Vol. 23, No. 3. pp. 1847-1857.
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abstract = "We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.",
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Kämäräinen, T, Arcot, LR, Johansson, L-S, Campbell, J, Tammelin, T, Franssila, S, Laine, J & Rojas, OJ 2016, 'UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers', Cellulose, vol. 23, no. 3, pp. 1847-1857. https://doi.org/10.1007/s10570-016-0942-x

UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers. / Kämäräinen, Tero; Arcot, Lokanathan R. (Corresponding Author); Johansson, Leena-Sisko; Campbell, Joseph; Tammelin, Tekla; Franssila, Sami; Laine, Janne; Rojas, Orlando, J.

In: Cellulose, Vol. 23, No. 3, 2016, p. 1847-1857.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - UV-ozone patterning of micro-nano fibrillated cellulose (MNFC) with alkylsilane self-assembled monolayers

AU - Kämäräinen, Tero

AU - Arcot, Lokanathan R.

AU - Johansson, Leena-Sisko

AU - Campbell, Joseph

AU - Tammelin, Tekla

AU - Franssila, Sami

AU - Laine, Janne

AU - Rojas, Orlando, J.

PY - 2016

Y1 - 2016

N2 - We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.

AB - We report on a facile photolithography-based procedure for surface energy patterning of novel micro-nano fibrillated cellulose (MNFC) films and demonstrate spatial control of protein adsorption. The kinetics of oxidative degradation of chemisorbed hydrophobic alkylsilane monolayers on MNFC upon exposure to UV/ozone and the effect on the adsorption of bovine serum albumin (BSA) as a function of pH were studied using surface sensitive techniques. Wetting properties, surface morphology and surface chemical composition of the MNFC films were investigated by using water contact angle goniometry, atomic force microscopy and X-ray photoelectron spectroscopy, respectively. Optical microscopy was used to give a spatial-specific visualization of adsorbed dye-tagged BSA. UV/ozone exposure turned the initially hydrophobic alkylsilane covered MNFC substrate into a hydrophilic surface. As a result, significant changes in local wetting characteristics were observed leading to a quantitative change in BSA adsorption. Moreover, by using a UV mask, it was possible to create a hydrophobic-hydrophilic pattern on the MNFC film, and thus spatially-resolved adsorption of protein patterns were achieved. These results extend the understanding and further the applicability of MNFC films towards microfluidic-based (bio)diagnostics.

KW - nanocellulose (MNFC)

KW - photolithography

KW - protein patterning

KW - self-assembled monolayer (SAM)

KW - silane

U2 - 10.1007/s10570-016-0942-x

DO - 10.1007/s10570-016-0942-x

M3 - Article

VL - 23

SP - 1847

EP - 1857

JO - Cellulose

JF - Cellulose

SN - 0969-0239

IS - 3

ER -