Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 179-189 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 727 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1999 |
| MoE publication type | A1 Journal article-refereed |
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Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection. / Erlund, Iris (Corresponding Author); Alfthan, Georg; Sirén, Heli; Ariniemi, Kari; Aro, Antti.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 727, No. 1-2, 1999, p. 179-189.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection
AU - Erlund, Iris
AU - Alfthan, Georg
AU - Sirén, Heli
AU - Ariniemi, Kari
AU - Aro, Antti
PY - 1999
Y1 - 1999
N2 - A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column and additional interferences were removed by extracting them into a toluene–dichloromethane mixture. The HPLC system consisted of an Inertsil ODS-3 column (250×4.0 mm) and a mobile phase with 59% methanol in phosphate buffer (pH 2.4). High selectivity and a low quantitation limit (0.63 μg/l) were achieved by using electrochemical detection at a low potential. The method has excellent reproducibility: R.S.D. values of peak-heights were 2% and 7.9%, respectively, for within-day and between-day precision. The method was applied to a small scale study of quercetin pharmacokinetics and quercetin was shown to be absorbed from a 20 mg dose. No free quercetin was detected in plasma and no evidence of significant amounts of quercetin glycosides in plasma was found.
AB - A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column and additional interferences were removed by extracting them into a toluene–dichloromethane mixture. The HPLC system consisted of an Inertsil ODS-3 column (250×4.0 mm) and a mobile phase with 59% methanol in phosphate buffer (pH 2.4). High selectivity and a low quantitation limit (0.63 μg/l) were achieved by using electrochemical detection at a low potential. The method has excellent reproducibility: R.S.D. values of peak-heights were 2% and 7.9%, respectively, for within-day and between-day precision. The method was applied to a small scale study of quercetin pharmacokinetics and quercetin was shown to be absorbed from a 20 mg dose. No free quercetin was detected in plasma and no evidence of significant amounts of quercetin glycosides in plasma was found.
U2 - 10.1016/S0378-4347(99)00049-3
DO - 10.1016/S0378-4347(99)00049-3
M3 - Article
VL - 727
SP - 179
EP - 189
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -