Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection

Iris Erlund (Corresponding Author), Georg Alfthan, Heli Siren, Kari Ariniemi, Antti Aro

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column and additional interferences were removed by extracting them into a toluene–dichloromethane mixture. The HPLC system consisted of an Inertsil ODS-3 column (250×4.0 mm) and a mobile phase with 59% methanol in phosphate buffer (pH 2.4). High selectivity and a low quantitation limit (0.63 μg/l) were achieved by using electrochemical detection at a low potential. The method has excellent reproducibility: R.S.D. values of peak-heights were 2% and 7.9%, respectively, for within-day and between-day precision. The method was applied to a small scale study of quercetin pharmacokinetics and quercetin was shown to be absorbed from a 20 mg dose. No free quercetin was detected in plasma and no evidence of significant amounts of quercetin glycosides in plasma was found.
Original languageEnglish
Pages (from-to)179-189
Number of pages11
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume727
Issue number1-2
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Plasma (human)
Quercetin
High performance liquid chromatography
High Pressure Liquid Chromatography
Pharmacokinetics
Plasmas
Biomarkers
Glycosides
Methanol
Blood Proteins
Buffers
Phosphates

Cite this

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title = "Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection",
abstract = "A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column and additional interferences were removed by extracting them into a toluene–dichloromethane mixture. The HPLC system consisted of an Inertsil ODS-3 column (250×4.0 mm) and a mobile phase with 59{\%} methanol in phosphate buffer (pH 2.4). High selectivity and a low quantitation limit (0.63 μg/l) were achieved by using electrochemical detection at a low potential. The method has excellent reproducibility: R.S.D. values of peak-heights were 2{\%} and 7.9{\%}, respectively, for within-day and between-day precision. The method was applied to a small scale study of quercetin pharmacokinetics and quercetin was shown to be absorbed from a 20 mg dose. No free quercetin was detected in plasma and no evidence of significant amounts of quercetin glycosides in plasma was found.",
author = "Iris Erlund and Georg Alfthan and Heli Siren and Kari Ariniemi and Antti Aro",
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language = "English",
volume = "727",
pages = "179--189",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1-2",

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Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection. / Erlund, Iris (Corresponding Author); Alfthan, Georg; Siren, Heli; Ariniemi, Kari; Aro, Antti.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 727, No. 1-2, 1999, p. 179-189.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Validated method for the quantitation of quercetin from human plasma using high-performance liquid chromatography with electrochemical detection

AU - Erlund, Iris

AU - Alfthan, Georg

AU - Siren, Heli

AU - Ariniemi, Kari

AU - Aro, Antti

PY - 1999

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AB - A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column and additional interferences were removed by extracting them into a toluene–dichloromethane mixture. The HPLC system consisted of an Inertsil ODS-3 column (250×4.0 mm) and a mobile phase with 59% methanol in phosphate buffer (pH 2.4). High selectivity and a low quantitation limit (0.63 μg/l) were achieved by using electrochemical detection at a low potential. The method has excellent reproducibility: R.S.D. values of peak-heights were 2% and 7.9%, respectively, for within-day and between-day precision. The method was applied to a small scale study of quercetin pharmacokinetics and quercetin was shown to be absorbed from a 20 mg dose. No free quercetin was detected in plasma and no evidence of significant amounts of quercetin glycosides in plasma was found.

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