Validation of the Hygicult (R) E dipslides method in surface hygiene control

A Nordic collaborative study

Satu Salo, T. Alanko, Anna-Maija Sjöberg, Gun Wirtanen

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

A collaborative study with Enterobacteriaceae was conducted to validate Hygicultr E dipslides by comparison with violet red bile glucose agar (VRBGA) contact plates and swabbing, using stainless steel surfaces artificially contaminated with microbes at various levels. Twelve laboratories participated in the validation procedure. The total number of collaborative samples was 108. The microbial level in each sample was assessed in triplicate by using the 3 above-mentioned methods. No Enterobacteriaceae were used at the low inoculation level. At the middle inoculation level, the percentages detached from the test surfaces were 16.6 with the Hygicult E method, 15.3 with the contact plate method, and 14.6 with swabbing; at the high innoculation level, the percentages were 14.5, 15.8, and 9.8, respectively. The percentage of acceptable results after the removal of outliers was 97.2. Repeatability relative standard deviations ranged from 33.4 to 44.9%; reproducibility relative standard deviations ranged from 45.2 to 77.1%. The Hygicult E dipslide, VRBGA contact plate, and swabbing methods gave similar results at all 3 microbial levels tested: <1.0 colony-forming units (CFU)/cm2 at the low level, 1.2-1.3 CFU/cm2 at the middle level (theoretical yield 8.0 CFU/cm2), and 1.2-2.0 CFU/cm2 at the high level (theoretical yield 12.5 CFU/cm2).
Original languageEnglish
Pages (from-to)388-394
JournalJournal of AOAC International
Volume85
Issue number2
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

hygiene
Hygiene
Agar
Stem Cells
Glucose
Stainless Steel
Enterobacteriaceae
bile
Bile
agar
inoculation
glucose
stainless steel
methodology
reproducibility
repeatability
outlier
microorganisms
sampling
steel

Keywords

  • Listeria monocytogenes
  • Salmonella typhimurium
  • Food microbiology
  • Stainless steel
  • Buna-N, Attachment
  • Systems, Design

Cite this

Salo, Satu ; Alanko, T. ; Sjöberg, Anna-Maija ; Wirtanen, Gun. / Validation of the Hygicult (R) E dipslides method in surface hygiene control : A Nordic collaborative study. In: Journal of AOAC International. 2002 ; Vol. 85, No. 2. pp. 388-394.
@article{ebeb932ae84041c59279205d9209cd06,
title = "Validation of the Hygicult (R) E dipslides method in surface hygiene control: A Nordic collaborative study",
abstract = "A collaborative study with Enterobacteriaceae was conducted to validate Hygicultr E dipslides by comparison with violet red bile glucose agar (VRBGA) contact plates and swabbing, using stainless steel surfaces artificially contaminated with microbes at various levels. Twelve laboratories participated in the validation procedure. The total number of collaborative samples was 108. The microbial level in each sample was assessed in triplicate by using the 3 above-mentioned methods. No Enterobacteriaceae were used at the low inoculation level. At the middle inoculation level, the percentages detached from the test surfaces were 16.6 with the Hygicult E method, 15.3 with the contact plate method, and 14.6 with swabbing; at the high innoculation level, the percentages were 14.5, 15.8, and 9.8, respectively. The percentage of acceptable results after the removal of outliers was 97.2. Repeatability relative standard deviations ranged from 33.4 to 44.9{\%}; reproducibility relative standard deviations ranged from 45.2 to 77.1{\%}. The Hygicult E dipslide, VRBGA contact plate, and swabbing methods gave similar results at all 3 microbial levels tested: <1.0 colony-forming units (CFU)/cm2 at the low level, 1.2-1.3 CFU/cm2 at the middle level (theoretical yield 8.0 CFU/cm2), and 1.2-2.0 CFU/cm2 at the high level (theoretical yield 12.5 CFU/cm2).",
keywords = "Listeria monocytogenes, Salmonella typhimurium, Food microbiology, Stainless steel, Buna-N, Attachment, Systems, Design",
author = "Satu Salo and T. Alanko and Anna-Maija Sj{\"o}berg and Gun Wirtanen",
year = "2002",
language = "English",
volume = "85",
pages = "388--394",
journal = "Journal of AOAC International",
issn = "1060-3271",
publisher = "AOAC International",
number = "2",

}

Validation of the Hygicult (R) E dipslides method in surface hygiene control : A Nordic collaborative study. / Salo, Satu; Alanko, T.; Sjöberg, Anna-Maija; Wirtanen, Gun.

In: Journal of AOAC International, Vol. 85, No. 2, 2002, p. 388-394.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Validation of the Hygicult (R) E dipslides method in surface hygiene control

T2 - A Nordic collaborative study

AU - Salo, Satu

AU - Alanko, T.

AU - Sjöberg, Anna-Maija

AU - Wirtanen, Gun

PY - 2002

Y1 - 2002

N2 - A collaborative study with Enterobacteriaceae was conducted to validate Hygicultr E dipslides by comparison with violet red bile glucose agar (VRBGA) contact plates and swabbing, using stainless steel surfaces artificially contaminated with microbes at various levels. Twelve laboratories participated in the validation procedure. The total number of collaborative samples was 108. The microbial level in each sample was assessed in triplicate by using the 3 above-mentioned methods. No Enterobacteriaceae were used at the low inoculation level. At the middle inoculation level, the percentages detached from the test surfaces were 16.6 with the Hygicult E method, 15.3 with the contact plate method, and 14.6 with swabbing; at the high innoculation level, the percentages were 14.5, 15.8, and 9.8, respectively. The percentage of acceptable results after the removal of outliers was 97.2. Repeatability relative standard deviations ranged from 33.4 to 44.9%; reproducibility relative standard deviations ranged from 45.2 to 77.1%. The Hygicult E dipslide, VRBGA contact plate, and swabbing methods gave similar results at all 3 microbial levels tested: <1.0 colony-forming units (CFU)/cm2 at the low level, 1.2-1.3 CFU/cm2 at the middle level (theoretical yield 8.0 CFU/cm2), and 1.2-2.0 CFU/cm2 at the high level (theoretical yield 12.5 CFU/cm2).

AB - A collaborative study with Enterobacteriaceae was conducted to validate Hygicultr E dipslides by comparison with violet red bile glucose agar (VRBGA) contact plates and swabbing, using stainless steel surfaces artificially contaminated with microbes at various levels. Twelve laboratories participated in the validation procedure. The total number of collaborative samples was 108. The microbial level in each sample was assessed in triplicate by using the 3 above-mentioned methods. No Enterobacteriaceae were used at the low inoculation level. At the middle inoculation level, the percentages detached from the test surfaces were 16.6 with the Hygicult E method, 15.3 with the contact plate method, and 14.6 with swabbing; at the high innoculation level, the percentages were 14.5, 15.8, and 9.8, respectively. The percentage of acceptable results after the removal of outliers was 97.2. Repeatability relative standard deviations ranged from 33.4 to 44.9%; reproducibility relative standard deviations ranged from 45.2 to 77.1%. The Hygicult E dipslide, VRBGA contact plate, and swabbing methods gave similar results at all 3 microbial levels tested: <1.0 colony-forming units (CFU)/cm2 at the low level, 1.2-1.3 CFU/cm2 at the middle level (theoretical yield 8.0 CFU/cm2), and 1.2-2.0 CFU/cm2 at the high level (theoretical yield 12.5 CFU/cm2).

KW - Listeria monocytogenes

KW - Salmonella typhimurium

KW - Food microbiology

KW - Stainless steel

KW - Buna-N, Attachment

KW - Systems, Design

M3 - Article

VL - 85

SP - 388

EP - 394

JO - Journal of AOAC International

JF - Journal of AOAC International

SN - 1060-3271

IS - 2

ER -