Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

J. J. Heikkinen, Liisa Kivimäki, J. E. Määttä, Inka Mäkelä, Leena Hakalahti, Kristiina Takkinen, Markku S. Kulomaa, V. P. Hytönen, O. E. O. Hormi (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume87
Issue number2
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Ink
Avidin
inks
Polymethyl Methacrylate
immobilization
Immobilization
Sol-gels
Silanes
Gels
gels
silanes
Substrates
Printing
biotin
cysteine
Biotin
Polymer films
printing
Cysteine
Methanol

Keywords

  • Biomolecule immobilization
  • Printing
  • Sol-gel
  • Maleimide
  • Chimeric avidin
  • Avidin-biotin technology
  • Biosensing

Cite this

Heikkinen, J. J. ; Kivimäki, Liisa ; Määttä, J. E. ; Mäkelä, Inka ; Hakalahti, Leena ; Takkinen, Kristiina ; Kulomaa, Markku S. ; Hytönen, V. P. ; Hormi, O. E. O. / Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. In: Colloids and Surfaces B: Biointerfaces. 2011 ; Vol. 87, No. 2. pp. 409-414.
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abstract = "A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.",
keywords = "Biomolecule immobilization, Printing, Sol-gel, Maleimide, Chimeric avidin, Avidin-biotin technology, Biosensing",
author = "Heikkinen, {J. J.} and Liisa Kivim{\"a}ki and M{\"a}{\"a}tt{\"a}, {J. E.} and Inka M{\"a}kel{\"a} and Leena Hakalahti and Kristiina Takkinen and Kulomaa, {Markku S.} and Hyt{\"o}nen, {V. P.} and Hormi, {O. E. O.}",
year = "2011",
doi = "10.1016/j.colsurfb.2011.05.052",
language = "English",
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Heikkinen, JJ, Kivimäki, L, Määttä, JE, Mäkelä, I, Hakalahti, L, Takkinen, K, Kulomaa, MS, Hytönen, VP & Hormi, OEO 2011, 'Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates', Colloids and Surfaces B: Biointerfaces, vol. 87, no. 2, pp. 409-414. https://doi.org/10.1016/j.colsurfb.2011.05.052

Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. / Heikkinen, J. J.; Kivimäki, Liisa; Määttä, J. E.; Mäkelä, Inka; Hakalahti, Leena; Takkinen, Kristiina; Kulomaa, Markku S.; Hytönen, V. P.; Hormi, O. E. O. (Corresponding Author).

In: Colloids and Surfaces B: Biointerfaces, Vol. 87, No. 2, 2011, p. 409-414.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

AU - Heikkinen, J. J.

AU - Kivimäki, Liisa

AU - Määttä, J. E.

AU - Mäkelä, Inka

AU - Hakalahti, Leena

AU - Takkinen, Kristiina

AU - Kulomaa, Markku S.

AU - Hytönen, V. P.

AU - Hormi, O. E. O.

PY - 2011

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N2 - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

AB - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

KW - Biomolecule immobilization

KW - Printing

KW - Sol-gel

KW - Maleimide

KW - Chimeric avidin

KW - Avidin-biotin technology

KW - Biosensing

U2 - 10.1016/j.colsurfb.2011.05.052

DO - 10.1016/j.colsurfb.2011.05.052

M3 - Article

VL - 87

SP - 409

EP - 414

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 2

ER -