Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

J. J. Heikkinen, Liisa Kivimäki, J. E. Määttä, Inka Mäkelä, Leena Hakalahti, Kristiina Takkinen, Markku S. Kulomaa, V. P. Hytönen, O. E. O. Hormi (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    13 Citations (Scopus)


    A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
    Original languageEnglish
    Pages (from-to)409-414
    Number of pages6
    JournalColloids and Surfaces B: Biointerfaces
    Issue number2
    Publication statusPublished - 2011
    MoE publication typeA1 Journal article-refereed


    • Biomolecule immobilization
    • Printing
    • Sol-gel
    • Maleimide
    • Chimeric avidin
    • Avidin-biotin technology
    • Biosensing


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