Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

J. J. Heikkinen; Liisa Kivimäki; J. E. Määttä; Inka Mäkelä; Leena Hakalahti; Kristiina Takkinen; Markku S. Kulomaa; V. P. Hytönen; O. E. O. Hormi

doi:10.1016/j.colsurfb.2011.05.052

Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

J. J. Heikkinen, Liisa Kivimäki, J. E. Määttä, Inka Mäkelä, Leena Hakalahti, Kristiina Takkinen, Markku S. Kulomaa, V. P. Hytönen, O. E. O. Hormi (Corresponding Author)

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

Original language	English
Pages (from-to)	409-414
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	87
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2011.05.052
Publication status	Published - 2011
MoE publication type	A1 Journal article-refereed

Keywords

Biomolecule immobilization
Printing
Sol-gel
Maleimide
Chimeric avidin
Avidin-biotin technology
Biosensing

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10.1016/j.colsurfb.2011.05.052

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title = "Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates",

abstract = "A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.",

keywords = "Biomolecule immobilization, Printing, Sol-gel, Maleimide, Chimeric avidin, Avidin-biotin technology, Biosensing",

author = "Heikkinen, {J. J.} and Liisa Kivim{\"a}ki and M{\"a}{\"a}tt{\"a}, {J. E.} and Inka M{\"a}kel{\"a} and Leena Hakalahti and Kristiina Takkinen and Kulomaa, {Markku S.} and Hyt{\"o}nen, {V. P.} and Hormi, {O. E. O.}",

year = "2011",

doi = "10.1016/j.colsurfb.2011.05.052",

language = "English",

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pages = "409--414",

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AU - Heikkinen, J. J.

AU - Kivimäki, Liisa

AU - Määttä, J. E.

AU - Mäkelä, Inka

AU - Hakalahti, Leena

AU - Takkinen, Kristiina

AU - Kulomaa, Markku S.

AU - Hytönen, V. P.

AU - Hormi, O. E. O.

PY - 2011

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N2 - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

AB - A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.

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KW - Printing

KW - Sol-gel

KW - Maleimide

KW - Chimeric avidin

KW - Avidin-biotin technology

KW - Biosensing

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DO - 10.1016/j.colsurfb.2011.05.052

M3 - Article

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 2

ER -

Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

Abstract

Keywords

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