A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.
- Biomolecule immobilization
- Chimeric avidin
- Avidin-biotin technology
Heikkinen, J. J., Kivimäki, L., Määttä, J. E., Mäkelä, I., Hakalahti, L., Takkinen, K., Kulomaa, M. S., Hytönen, V. P., & Hormi, O. E. O. (2011). Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates. Colloids and Surfaces B: Biointerfaces, 87(2), 409-414. https://doi.org/10.1016/j.colsurfb.2011.05.052