Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

M. Jämsä, S. Kosourov, V. Rissanen, M. Hakalahti, J. Pere, J. A. Ketoja, T. Tammelin (Corresponding Author), Y. Allahverdiyeva (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

Versatile templates were fabricated using plant-derived nanomaterials, TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) for the efficient and sustainable production of biofuels from cyanobacteria and green algae. We used three different approaches to immobilize the model filamentous cyanobacteria or green algae to the TEMPO CNF matrix. These approaches involved the fabrication of: (A) a pure TEMPO CNF hydrogel; (B) a Ca2+-stabilized TEMPO CNF hydrogel; and (C) a solid TEMPO CNF film, which was crosslinked with polyvinyl alcohol (PVA). The different immobilization approaches resulted in matrices with enhanced water stability performance. In all cases, the photosynthetic activity and H2 photoproduction capacity of cyanobacteria and algae entrapped in TEMPO CNF were comparable to a conventional alginate-based matrix. Green algae entrapped in Ca2+-stabilized TEMPO CNF hydrogels showed even greater rates of H2 production than control alginate-entrapped algae under the more challenging submerged cultivation condition. Importantly, cyanobacterial filaments entrapped within dried TEMPO CNF films showed full recovery once rewetted, and they continued efficient H2 production. The immobilization mechanism was passive entrapment, which was directly evidenced using surface sensitive quartz crystal microbalance with dissipation monitoring (QCM-D). The results obtained demonstrate a high compatibility between CNF and photosynthetic microbes. This opens new possibilities for developing a novel technology platform based on CNF templates with tailored pore-size and controllable surface charges that target sustainable chemical production by oxygenic photosynthetic microorganisms.

Original languageEnglish
Pages (from-to)5825-5835
Number of pages11
JournalJournal of Materials Chemistry A: Materials for Energy and Sustainability
Volume6
Issue number14
DOIs
Publication statusPublished - 14 Apr 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

oxidized cellulose
Biofuels
Cellulose
Algae
Hydrogels
Cellulose films
Alginate
Hydrogel
Production control
Quartz crystal microbalances
Polyvinyl alcohols
Surface charge
Nanostructured materials
Polyvinyl Alcohol
Microorganisms
Pore size
TEMPO
Fabrication
Recovery
Monitoring

Cite this

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title = "Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production",
abstract = "Versatile templates were fabricated using plant-derived nanomaterials, TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) for the efficient and sustainable production of biofuels from cyanobacteria and green algae. We used three different approaches to immobilize the model filamentous cyanobacteria or green algae to the TEMPO CNF matrix. These approaches involved the fabrication of: (A) a pure TEMPO CNF hydrogel; (B) a Ca2+-stabilized TEMPO CNF hydrogel; and (C) a solid TEMPO CNF film, which was crosslinked with polyvinyl alcohol (PVA). The different immobilization approaches resulted in matrices with enhanced water stability performance. In all cases, the photosynthetic activity and H2 photoproduction capacity of cyanobacteria and algae entrapped in TEMPO CNF were comparable to a conventional alginate-based matrix. Green algae entrapped in Ca2+-stabilized TEMPO CNF hydrogels showed even greater rates of H2 production than control alginate-entrapped algae under the more challenging submerged cultivation condition. Importantly, cyanobacterial filaments entrapped within dried TEMPO CNF films showed full recovery once rewetted, and they continued efficient H2 production. The immobilization mechanism was passive entrapment, which was directly evidenced using surface sensitive quartz crystal microbalance with dissipation monitoring (QCM-D). The results obtained demonstrate a high compatibility between CNF and photosynthetic microbes. This opens new possibilities for developing a novel technology platform based on CNF templates with tailored pore-size and controllable surface charges that target sustainable chemical production by oxygenic photosynthetic microorganisms.",
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Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production. / Jämsä, M.; Kosourov, S.; Rissanen, V.; Hakalahti, M.; Pere, J.; Ketoja, J. A.; Tammelin, T. (Corresponding Author); Allahverdiyeva, Y. (Corresponding Author).

In: Journal of Materials Chemistry A: Materials for Energy and Sustainability, Vol. 6, No. 14, 14.04.2018, p. 5825-5835.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

AU - Jämsä, M.

AU - Kosourov, S.

AU - Rissanen, V.

AU - Hakalahti, M.

AU - Pere, J.

AU - Ketoja, J. A.

AU - Tammelin, T.

AU - Allahverdiyeva, Y.

PY - 2018/4/14

Y1 - 2018/4/14

N2 - Versatile templates were fabricated using plant-derived nanomaterials, TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) for the efficient and sustainable production of biofuels from cyanobacteria and green algae. We used three different approaches to immobilize the model filamentous cyanobacteria or green algae to the TEMPO CNF matrix. These approaches involved the fabrication of: (A) a pure TEMPO CNF hydrogel; (B) a Ca2+-stabilized TEMPO CNF hydrogel; and (C) a solid TEMPO CNF film, which was crosslinked with polyvinyl alcohol (PVA). The different immobilization approaches resulted in matrices with enhanced water stability performance. In all cases, the photosynthetic activity and H2 photoproduction capacity of cyanobacteria and algae entrapped in TEMPO CNF were comparable to a conventional alginate-based matrix. Green algae entrapped in Ca2+-stabilized TEMPO CNF hydrogels showed even greater rates of H2 production than control alginate-entrapped algae under the more challenging submerged cultivation condition. Importantly, cyanobacterial filaments entrapped within dried TEMPO CNF films showed full recovery once rewetted, and they continued efficient H2 production. The immobilization mechanism was passive entrapment, which was directly evidenced using surface sensitive quartz crystal microbalance with dissipation monitoring (QCM-D). The results obtained demonstrate a high compatibility between CNF and photosynthetic microbes. This opens new possibilities for developing a novel technology platform based on CNF templates with tailored pore-size and controllable surface charges that target sustainable chemical production by oxygenic photosynthetic microorganisms.

AB - Versatile templates were fabricated using plant-derived nanomaterials, TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) for the efficient and sustainable production of biofuels from cyanobacteria and green algae. We used three different approaches to immobilize the model filamentous cyanobacteria or green algae to the TEMPO CNF matrix. These approaches involved the fabrication of: (A) a pure TEMPO CNF hydrogel; (B) a Ca2+-stabilized TEMPO CNF hydrogel; and (C) a solid TEMPO CNF film, which was crosslinked with polyvinyl alcohol (PVA). The different immobilization approaches resulted in matrices with enhanced water stability performance. In all cases, the photosynthetic activity and H2 photoproduction capacity of cyanobacteria and algae entrapped in TEMPO CNF were comparable to a conventional alginate-based matrix. Green algae entrapped in Ca2+-stabilized TEMPO CNF hydrogels showed even greater rates of H2 production than control alginate-entrapped algae under the more challenging submerged cultivation condition. Importantly, cyanobacterial filaments entrapped within dried TEMPO CNF films showed full recovery once rewetted, and they continued efficient H2 production. The immobilization mechanism was passive entrapment, which was directly evidenced using surface sensitive quartz crystal microbalance with dissipation monitoring (QCM-D). The results obtained demonstrate a high compatibility between CNF and photosynthetic microbes. This opens new possibilities for developing a novel technology platform based on CNF templates with tailored pore-size and controllable surface charges that target sustainable chemical production by oxygenic photosynthetic microorganisms.

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U2 - 10.1039/c7ta11164a

DO - 10.1039/c7ta11164a

M3 - Article

VL - 6

SP - 5825

EP - 5835

JO - Journal of Materials Chemistry A: Materials for Energy and Sustainability

JF - Journal of Materials Chemistry A: Materials for Energy and Sustainability

SN - 2050-7488

IS - 14

ER -