Viability of bovine embryos following exposure to the green filtered or wider bandwidth light during in vitro embryo production

Kati Korhonen (Corresponding Author), Sami Sjövall, Jouko Viitanen, Elise Ketoja, Alexander Makarevich, Jaana Peippo

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

BACKGROUND

Microscopic light during in vitro production (IVP) is a common stress factor compromising embryo development and viability. Many studies discussing detrimental effects of light have been conducted on in vivo matured/fertilized oocytes or on flushed embryos that were exposed to light only when cultured in vitro. The aim of this work was to examine the effects of light composition during all IVP steps on subsequent embryo development and quality.

METHODS

We compared the effects of a green pass filter of 498–563 nm wavelength, and a wider bandwidth of stereomicroscopic light on bovine embryo development rates, total cell counts and the presence of constitutive (Hsp73) and stress-inducible (Hsp72) forms of the Hsp70 protein.

RESULTS

The use of the green filter had no effect on embryo development rates, morphological quality or total cell counts on Day 7 or 8 of development compared with control group. However, Hsp72/73 protein levels revealed the protective effect of the filter against harmful blue and infrared regions of the light. The constitutive form Hsp73 was seen in both groups, but the inducible stress-response form Hsp72 was absent from the filter group embryos and appeared only in the group exposed to the stereomicroscopic light.

CONCLUSIONS

An easy to use and inexpensive green filter seems to reduce the stress caused by light during the IVP procedures without affecting either the accuracy of embryo monitoring or the need to increase the light intensity.

Original languageEnglish
Pages (from-to)308-314
JournalHuman Reproduction
Volume24
Issue number2
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Embryonic Structures
Light
Embryonic Development
Cell Count
HSP72 Heat-Shock Proteins
In Vitro Techniques
Green Or
Oocytes
Control Groups
Proteins

Keywords

  • Green filter
  • Hsp70
  • IVC
  • IVF
  • IVM
  • Light-induced stress

Cite this

Korhonen, Kati ; Sjövall, Sami ; Viitanen, Jouko ; Ketoja, Elise ; Makarevich, Alexander ; Peippo, Jaana. / Viability of bovine embryos following exposure to the green filtered or wider bandwidth light during in vitro embryo production. In: Human Reproduction. 2009 ; Vol. 24, No. 2. pp. 308-314.
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abstract = "BACKGROUNDMicroscopic light during in vitro production (IVP) is a common stress factor compromising embryo development and viability. Many studies discussing detrimental effects of light have been conducted on in vivo matured/fertilized oocytes or on flushed embryos that were exposed to light only when cultured in vitro. The aim of this work was to examine the effects of light composition during all IVP steps on subsequent embryo development and quality.METHODSWe compared the effects of a green pass filter of 498–563 nm wavelength, and a wider bandwidth of stereomicroscopic light on bovine embryo development rates, total cell counts and the presence of constitutive (Hsp73) and stress-inducible (Hsp72) forms of the Hsp70 protein.RESULTSThe use of the green filter had no effect on embryo development rates, morphological quality or total cell counts on Day 7 or 8 of development compared with control group. However, Hsp72/73 protein levels revealed the protective effect of the filter against harmful blue and infrared regions of the light. The constitutive form Hsp73 was seen in both groups, but the inducible stress-response form Hsp72 was absent from the filter group embryos and appeared only in the group exposed to the stereomicroscopic light.CONCLUSIONSAn easy to use and inexpensive green filter seems to reduce the stress caused by light during the IVP procedures without affecting either the accuracy of embryo monitoring or the need to increase the light intensity.",
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Viability of bovine embryos following exposure to the green filtered or wider bandwidth light during in vitro embryo production. / Korhonen, Kati (Corresponding Author); Sjövall, Sami; Viitanen, Jouko; Ketoja, Elise; Makarevich, Alexander; Peippo, Jaana.

In: Human Reproduction, Vol. 24, No. 2, 2009, p. 308-314.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Viability of bovine embryos following exposure to the green filtered or wider bandwidth light during in vitro embryo production

AU - Korhonen, Kati

AU - Sjövall, Sami

AU - Viitanen, Jouko

AU - Ketoja, Elise

AU - Makarevich, Alexander

AU - Peippo, Jaana

PY - 2009

Y1 - 2009

N2 - BACKGROUNDMicroscopic light during in vitro production (IVP) is a common stress factor compromising embryo development and viability. Many studies discussing detrimental effects of light have been conducted on in vivo matured/fertilized oocytes or on flushed embryos that were exposed to light only when cultured in vitro. The aim of this work was to examine the effects of light composition during all IVP steps on subsequent embryo development and quality.METHODSWe compared the effects of a green pass filter of 498–563 nm wavelength, and a wider bandwidth of stereomicroscopic light on bovine embryo development rates, total cell counts and the presence of constitutive (Hsp73) and stress-inducible (Hsp72) forms of the Hsp70 protein.RESULTSThe use of the green filter had no effect on embryo development rates, morphological quality or total cell counts on Day 7 or 8 of development compared with control group. However, Hsp72/73 protein levels revealed the protective effect of the filter against harmful blue and infrared regions of the light. The constitutive form Hsp73 was seen in both groups, but the inducible stress-response form Hsp72 was absent from the filter group embryos and appeared only in the group exposed to the stereomicroscopic light.CONCLUSIONSAn easy to use and inexpensive green filter seems to reduce the stress caused by light during the IVP procedures without affecting either the accuracy of embryo monitoring or the need to increase the light intensity.

AB - BACKGROUNDMicroscopic light during in vitro production (IVP) is a common stress factor compromising embryo development and viability. Many studies discussing detrimental effects of light have been conducted on in vivo matured/fertilized oocytes or on flushed embryos that were exposed to light only when cultured in vitro. The aim of this work was to examine the effects of light composition during all IVP steps on subsequent embryo development and quality.METHODSWe compared the effects of a green pass filter of 498–563 nm wavelength, and a wider bandwidth of stereomicroscopic light on bovine embryo development rates, total cell counts and the presence of constitutive (Hsp73) and stress-inducible (Hsp72) forms of the Hsp70 protein.RESULTSThe use of the green filter had no effect on embryo development rates, morphological quality or total cell counts on Day 7 or 8 of development compared with control group. However, Hsp72/73 protein levels revealed the protective effect of the filter against harmful blue and infrared regions of the light. The constitutive form Hsp73 was seen in both groups, but the inducible stress-response form Hsp72 was absent from the filter group embryos and appeared only in the group exposed to the stereomicroscopic light.CONCLUSIONSAn easy to use and inexpensive green filter seems to reduce the stress caused by light during the IVP procedures without affecting either the accuracy of embryo monitoring or the need to increase the light intensity.

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KW - Hsp70

KW - IVC

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KW - IVM

KW - Light-induced stress

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