Vibrational spectroscopy and its future applications in microbiology

Miia Marika Jansson, Martin Kögler, Sohvi Hörkkö, Tero Ala-Kokko, Lassi Rieppo

Research output: Contribution to journalReview Articlepeer-review

4 Citations (Scopus)


Vibrational spectroscopic techniques, namely Fourier transform infrared (FTIR) and Raman spectroscopy, are based on the study of molecular vibrations, and they are complementary techniques to each other. This review provides an overview of the vibrational spectroscopic techniques applied in microbiology during the past decade. In addition, future applications of the elaborated spectroscopic techniques will be highlighted. The results of this review show that both FTIR and Raman spectroscopy are promising alternatives to conventional diagnostic approaches because they provide label-free and noninvasive bacterial detection, identification, and antibiotic susceptibility testing in a single step. Cost-effective, accurate, and rapid tests are needed in order to improve diagnostics and patient care, to decrease the use of unnecessary antimicrobial agents, to prevent resistant microbials, and to decrease the overall burden of outbreaks. Prior to that, however, the presented approaches need to be validated in a clinical workflow against the conventional diagnostic approaches.
Original languageEnglish
Pages (from-to)132-158
Number of pages27
JournalApplied Spectroscopy Reviews
Issue number2
Publication statusPublished - 2023
MoE publication typeA2 Review article in a scientific journal


  • Fourier transform infrared spectroscopy; microbiology; Raman spectroscopy; time- gated Raman spectroscopy; surface-enhanced Raman spectroscopy
  • time-gated Raman spectroscopy
  • microbiology
  • Fourier transform infrared spectroscopy
  • Raman spectroscopy
  • surface-enhanced Raman spectroscopy


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